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Revision as of 10:20, 14 September 2013
The production system
The micro-road to rubber
Natural rubber is a chain of several isoprene molecules. The rubber tree has a prenyltransferase (HRT2) enzyme, which links molecules of IPP (Isopentenyl pyrophosphate) into the isoprene chain (natural rubber). To start the extension of the chain, the prenyltransferase needs a DMAPP (Dimethylallyl pyrophosphate) molecule. Thus, in order to produce rubber, the bacteria needs the prenyltranferase, IPP, and DMAPP.
IPP and DMAPP are molecules naturally present in E. coli, produced by the bacteria through the MEP pathway (Methylerythritol phosphate pathway). The MEP pathway converts molecules of GAP (Glyceraldehyde-3-phosphate) and pyruvate - produced by the degradation of glucose (glycolysis) - into IPP and DMAPP. To increase the amount of IPP and DMAPP available for rubber production, we optimized the MEP pathway by elevating the levels of rate limiting enzymes in the pathway.
Through gene manipulation, we introduced the prenyltransferase gene from the rubber tree to the genetic code of E. coli. This enables the bacteria to express the prenyltransferase enzyme. Consequently the bacteria should be able to convert sugar into natural rubber through; the glycolysis, the MEP pathway, and the prenyltransferase activity.
Dig deeper to see the specification and models of our system, how we designed it in detail, and what thoughts we have about safety.
