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Team:NCTU Formosa/safety
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===SAFETY PROCEDURE IN OUR LABORATORY=== | ===SAFETY PROCEDURE IN OUR LABORATORY=== | ||
| + | This year, we use E.coli DH5α, which is classified under Risk group 1, as our It's a non-hazardous and non-infective bacteria, and generally used in labs | ||
====Instruments and equipment in Our Laboratory==== | ====Instruments and equipment in Our Laboratory==== | ||
Revision as of 07:41, 19 September 2013
Inside a newly built, well-equipped laboratory, we conducted our experiment. Besides safety equipment, each member is well informed of the safety rules and emergency handling.
Contents |
SAFETY OF OUR PROJECT
Our project is to create a regulation system of gene expression in bacteria, only create new biobrick without producing any biosynthesis product. So it won't have new risks if we used our part in commerce or industry.We had discussed with National Chiao Tung University Environmental Protection and Safety Center.The committee agree us executing our project without any comment.
ORGANISMS WE USED
| Species | Strain Number | Risk group | Disease risk to human |
|---|---|---|---|
| E.coli | DH5α | 1 | May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. |
NEW OR MODIFIED CODING REGION WE USED
| Part number | Where to get this part | Originally come from | Risk group | Function |
|---|---|---|---|---|
| BBa_K1017301 | addgene | Synechocystis | 1 | chimeric light receptor function of the photoreceptor Cph1 |
| BBa_K1017301 | genedireX | E. coli | 1 | egulating gene expression |
| BBa_K1017302 | genedireX | E. coli | 1 | egulating gene expression |
SAFETY PROCEDURE IN OUR LABORATORY
This year, we use E.coli DH5α, which is classified under Risk group 1, as our It's a non-hazardous and non-infective bacteria, and generally used in labs
Instruments and equipment in Our Laboratory
 Figure.1 Biosafety cabinet |
 Figure.2 4℃ Refrigerator |
 Figure.3 Sterilizer |
Researcher's Safety
In order to protect researcher from infecting engineered organisms or contaminating the samples, we use the following procedures:
- Researchers who are doing experiment must wear on lab coat and gloves.
Figure.5 Researcher's wearing(待補) - No experiments is allowed to be done outside the biosafety cabinet.
Pubilc Safety
The organism we used is E.coli DH5α, which is classified under Risk group 1. It's a non-hazardous and non-infective bacteria. To ensure that the engineered organisms will not leak out to the environment, all wastes which contacted these bacteria should be sterilized before disposal.
