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From 2013.igem.org

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-	<div style="font-family:arial;padding:5px;border-radius:5px;border:5px solid #FF2800;">	+	==DNA isolation from PCR products==
-	{| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center"	+	*Isolating DNA using Omega nucleic acid purification kit and following its protocol.
-	!align="center"|[[Team:Leicester|Home]]	+	*Isolated DNA from all of the samples B and F and samples X3, 5, 6, 7, 8, 10, 12.
-	!align="center"|[[Team:Leicester/Team|Team]]	+	*Selection was made by looking at gel pictures and selecting samples with visible bands.
-	!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile]	+
-	!align="center"|[[Team:Leicester/Project|Project]]	+	==Nanodropping the samples for concentration==
-	!align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]]	+	{|border=1
-	!align="center"|[[Team:Leicester/Modeling|Modeling]]	+	|Sample nr||Volume ul||Concentration ng/ul||260/280||260/230
-	!align="center"|[[Team:Leicester/Notebook|Notebook]]	+	|-
-	!align="center"|[[Team:Leicester/Safety|Safety]]	+	|
-	!align="center"|[[Team:Leicester/Attributions|Attributions]]	+
-	|}	+	==Sending isolated DNA to be sequenced by PNACL==
-	</div>	+	*Sending 4 samples from each operon that showed highest DNA concentration: B5, 4, 9, 12; F8, 1, 10, 12; X3, 5, 6, 12.
		+
		+	==Preparing overnight broths==
		+	*Preparing broths from individual colonie plates from previous day
		+	*Selecting 4 colonies from each that showed highest DNA concentration from nanodrop: B5, 4, 9, 12; F8, 1, 10, 12; X3, 5, 6, 12.

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Revision as of 09:57, 20 September 2013

Contents 1 DNA isolation from PCR products 2 Nanodropping the samples for concentration 3 Sending isolated DNA to be sequenced by PNACL 4 Preparing overnight broths

DNA isolation from PCR products

• Isolating DNA using Omega nucleic acid purification kit and following its protocol.
• Isolated DNA from all of the samples B and F and samples X3, 5, 6, 7, 8, 10, 12.
• Selection was made by looking at gel pictures and selecting samples with visible bands.

Nanodropping the samples for concentration

Sample nr	Volume ul	Concentration ng/ul	260/280	260/230
Sending isolated DNA to be sequenced by PNACL Sending 4 samples from each operon that showed highest DNA concentration: B5, 4, 9, 12; F8, 1, 10, 12; X3, 5, 6, 12. Preparing overnight broths Preparing broths from individual colonie plates from previous day Selecting 4 colonies from each that showed highest DNA concentration from nanodrop: B5, 4, 9, 12; F8, 1, 10, 12; X3, 5, 6, 12.