19/09/13

(Difference between revisions)

Revision as of 10:48, 20 September 2013

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Isolating DNA using Omega nucleic acid purification kit and following its protocol.
Isolated DNA from all of the samples B and F and samples X3, 5, 6, 7, 8, 10, 12.
Selection was made by looking at gel pictures and selecting samples with visible bands.

Sending 4 samples from each operon that showed highest DNA concentration: B5, 4, 9, 12; F8, 1, 10, 12; X3, 5, 6, 12.

Preparing broths from individual colonie plates from previous day
Selecting 4 colonies from each that showed highest DNA concentration from nanodrop: B5, 4, 9, 12; F8, 1, 10, 12; X3, 5, 6, 12.

@@ Line 22: / Line 22: @@
 |Sample nr||Volume ul||Concentration ng/ul||260/280||260/230
 |-
-|X3||11.3||1.87||1.29
+|X3||41||11.3||1.87||1.29
 |-
-|X5||12.2||1.78||1.22
+|X5||47||12.2||1.78||1.22
 |-
-|X6||12.9||1.79||1.22
+|X6||34||12.9||1.79||1.22
 |-
-|X7||8||1.71||0.96
+|X7||45||8||1.71||0.96
 |-
-|X8||10.5||1.88||1.2
+|X8||43||10.5||1.88||1.2
+|-
+|X10||44||9||1.88||1.2
+|-
+|X12||45||12.8||1.70||1.40
+|-
+|F1||44.5||11.2||1.69||1.22
+|-
+|F2||44||9.1||1.88||1.20
+|-
+|F3||45.2||10.6||1.77||0.99
+|-
+|F4||43.7||9.7||1.82||1.10
+|-
+|F5||45.2||10.6||1.67||1.19
+|-
+|F6||45.4||9.6||1.82||0.85
+|-
+|F7||46||11||1.76||1.14
+|-
+|F8||46||11.7||1.73||1.24
+|-
+|F9||42.4||10.5||1.83||1.32
+|-
+|F10||44.3||11||1.93||1.39
+|-
+|F11||45.5||10.3||1.82||1.18
+|-
+|F12||45.5||14.9||1.83||1.32
+|-
+|B1||45||6.9||1.56||0.86
+|-
+|B2||46||10.5||1.64||1.17
+|-
+|B3||46||10||1.78||1.41
+|-
+|B4||47||11.5||1.70||1.35
+|-
+|B5||47||10.4||1.84||1.25
+|-
+|B6||47||8.6||1.96||1.33
+|-
+|B7||47||9.7||1.74||1.01
+|-
+|B8||46||10.7||1.86|1.22
+|-
+|B9||45||12.4||1.58||1.05
+|-
+|B10||46||11.1||1.73||1.32
+|-
+|B11||46||10.2||1.87||1.56
+|-
+|B12||46||12.1||1.75||1.38
 |}