Team:BYU Provo/Notebook/Cholera - Detection/Fallexp

From 2013.igem.org

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<font size="3" font face="Calibri"> We discovered that lambda is rendered ineffective after electrporation. The children's book for our outreach project was completed. We also streaked cholera next to our lambda containing e. coli and discovered that cholera induces the lysis of our lambda phage. </font>
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<font size="3" font face="Calibri"> We discovered that lambda is rendered ineffective after electrporation. The children's book for our outreach project was completed. We also streaked cholera next to our lambda containing e. coli and discovered that cholera induces the lysis of our lambda phage. Our first attempts at purifying lambda phage. </font>
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<font size="3" font face="Calibri"> We did further work on purifying lambda. Also, we have been working on mutating a membrane protein called SdiA. It recognizes cholera's AHL. We will use it to enable our construct to only respond to cholera's AHLs instead of all AHLs. </font>
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Revision as of 02:58, 21 September 2013


Cholera Detection September Notebook



Cholera Detection
March-April
May-June
July-August
September-October

September 2 - September 15


We discovered that lambda is rendered ineffective after electrporation. The children's book for our outreach project was completed. We also streaked cholera next to our lambda containing e. coli and discovered that cholera induces the lysis of our lambda phage. Our first attempts at purifying lambda phage.


Daily log



DetectionSpring1.JPG


September 16 - September 29


We did further work on purifying lambda. Also, we have been working on mutating a membrane protein called SdiA. It recognizes cholera's AHL. We will use it to enable our construct to only respond to cholera's AHLs instead of all AHLs.


Daily log



BYUCDFall2.JPG