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Team:NCTU Formosa/results
From 2013.igem.org
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===37 degrees Celsius RBS=== | ===37 degrees Celsius RBS=== | ||
| - | <p>Using Pcons+37°C RBS+mGFP+J61048, we tested the 37°C RBS | + | <p>Using biobrick, Pcons+37°C RBS+mGFP+J61048, we tested the function of the 37°C RBS under different temperatures.</p> |
| - | [[File:NCTU_37RBS_result.png|center|600px|Fig 3. 37°C RBS efficiency | + | [[File:NCTU_37RBS_result.png|center|600px|Fig 3. 37°C RBS efficiency under different temperatures.]] |
| - | <span class="undetermined"> | + | <span class="undetermined">As shown on Fig 3. the expression of mGFP under 37°C and 42°C reached a higher OD value in less time than the expression below 37°C. This demonstrates the fact that 37°C RBS functions more efficiently above or at 37°C. On the other hand, the fact that 37°C RBS leads to moderate expression even under 37°C degree might be a problem, since then we cannot make sure that only single gene is expressed under 37°C.</span> |
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Revision as of 09:16, 21 September 2013
The current progress of our project, including detailed information of the experimental data and the overall evaluation of the practicability of this project.
Contents |
RBS efficiency
We used the following biobrick to test the efficiency of different ribosome binding sites:
- Pcons+BBa_B0034+mRFP+Ter
- Pcons+[http://parts.igem.org/wiki/index.php?title=Part:BBa_K1017202 BBa_K1017202]+mRFP+Ter
- Pcons+BBa_B0030+mRFP+Ter
- Pcons+BBa_B0032+mRFP+Ter
- control:pet40
The control is pet40 and the reporter gene is mRFP, Figure 1 and Figure 2 show the difference of color.
(undetermined)
37 degrees Celsius RBS
Using biobrick, Pcons+37°C RBS+mGFP+J61048, we tested the function of the 37°C RBS under different temperatures.
As shown on Fig 3. the expression of mGFP under 37°C and 42°C reached a higher OD value in less time than the expression below 37°C. This demonstrates the fact that 37°C RBS functions more efficiently above or at 37°C. On the other hand, the fact that 37°C RBS leads to moderate expression even under 37°C degree might be a problem, since then we cannot make sure that only single gene is expressed under 37°C.
