Template:Kyoto/Notebook/Aug 10
From 2013.igem.org
(Difference between revisions)
(→Ligation) |
(→Ligation) |
||
Line 6: | Line 6: | ||
!state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2 | !state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2 | ||
|- | |- | ||
- | |experiment|| | + | |experiment||Pconst||13.2||spiinach-DT||2.1L||3.5 |
|- | |- | ||
- | |experiment|| | + | |experiment||Plac||15.3||spiinach-DT||2.1||3.5 |
|- | |- | ||
- | |experiment|| | + | |experiment||Pconst||13.2||pT181 antisense (XbaI & PstI)||6.6||3.5 |
|- | |- | ||
- | |experiment|| | + | |experiment||Plac||15.3||pT181 antisense (XbaI & PstI)||6.6||3.5 |
|- | |- | ||
- | |experiment|| | + | |experiment||Ptet||30.1||pT181 antisense (XbaI & PstI)||6.6||3.5 |
|- | |- | ||
- | |experiment|| | + | |experiment||Pconst||13.2||pT181 attenuator||11.7||3.5 |
|- | |- | ||
- | |experiment|| | + | |experiment||Plac||15.3||pT181 attenuator||11.7||3.5 |
|- | |- | ||
- | |experiment|| | + | |experiment||Ptet||30.1||RBS-lacZα-DT||3.0||3.5 |
|- | |- | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
|} | |} | ||
+ | incubate 16°C 1hour | ||
</div> | </div> |
Revision as of 10:12, 23 September 2013
Aug 10
Ligation
state | Vector | Inserter | Ligation High ver.2 | ||
---|---|---|---|---|---|
experiment | Pconst | 13.2 | spiinach-DT | 2.1L | 3.5 |
experiment | Plac | 15.3 | spiinach-DT | 2.1 | 3.5 |
experiment | Pconst | 13.2 | pT181 antisense (XbaI & PstI) | 6.6 | 3.5 |
experiment | Plac | 15.3 | pT181 antisense (XbaI & PstI) | 6.6 | 3.5 |
experiment | Ptet | 30.1 | pT181 antisense (XbaI & PstI) | 6.6 | 3.5 |
experiment | Pconst | 13.2 | pT181 attenuator | 11.7 | 3.5 |
experiment | Plac | 15.3 | pT181 attenuator | 11.7 | 3.5 |
experiment | Ptet | 30.1 | RBS-lacZα-DT | 3.0 | 3.5 |
incubate 16°C 1hour