Team:Tsinghua-E/Notebook

From 2013.igem.org

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<li><a href="https://2013.igem.org/Team:Tsinghua-E/Notebook">Diary</a></li>
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<li><a href="https://2013.igem.org/Team:Tsinghua-E/Notebook">Journal</a></li>
<li><a href="https://2013.igem.org/Team:Tsinghua-E/Notebook">Protocols</a></li>
<li><a href="https://2013.igem.org/Team:Tsinghua-E/Notebook">Protocols</a></li>
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<p>Notice:Except for specially mentioned, all the following vectors were constructed by In-Fusion HD Cloning Kit (produced by Clontech). To be brief, every fragment of desired DNA was linearized by primers designed following the protocol provided by Takara infusion cloning guide to ensure accurately 15 base pair (bp) overlap homology with each adjacent fragments. The gel-purified fragments were mixed with 2uL infusion cloning kit Premix solution and ddH2O was used to adjust the mixture to 10uL. The amount of the added fragments was based on the Takara infusion cloning guide.</p>
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  <p>    You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.</p>
 
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== '''Week1''' ==
== '''Week1''' ==
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== '''Week10''' ==
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Revision as of 10:12, 23 September 2013

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Notice:Except for specially mentioned, all the following vectors were constructed by In-Fusion HD Cloning Kit (produced by Clontech). To be brief, every fragment of desired DNA was linearized by primers designed following the protocol provided by Takara infusion cloning guide to ensure accurately 15 base pair (bp) overlap homology with each adjacent fragments. The gel-purified fragments were mixed with 2uL infusion cloning kit Premix solution and ddH2O was used to adjust the mixture to 10uL. The amount of the added fragments was based on the Takara infusion cloning guide.

Contents

Week1

Week2

Week3

Week4

Week5

Week6

Week7

Week8

Week9

Week10

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