Template:Team:Bielefeld-Germany/Notebook/Week02

From 2013.igem.org

(Difference between revisions)
m
m
Line 21: Line 21:
</style>
</style>
<div id="page-content">
<div id="page-content">
-
 
-
<div class="article" id="w02-13-05">
 
-
<div class="article-title">
 
-
<p><a name="week02-13-05">Week 2, 13.05.2013, Monday</a></p>
 
-
</div>
 
-
<div class="article-details">
 
-
<p>Posted on 10/06/2013</p>
 
-
</div>
 
-
<div class="article-text">
 
-
 
-
                <p>
 
-
<b>1) Experiment 1</b><br/>
 
-
We did it!<br/><br/>
 
-
 
-
<b>2) Experiment 2</b><br/>
 
-
                Succeeded!
 
-
 
-
</p>
 
-
</div><!-- /article-text -->
 
-
</div><!-- /article -->
 
-
 
Line 56: Line 35:
                 - Preparing overnight culture ''E. coli'' KRX <br/><br/>
                 - Preparing overnight culture ''E. coli'' KRX <br/><br/>
-
<b>2) Experiment 2 - Incubation</b><br/>
 
-
Description of experiment 2
 
-
                <br>
 
-
<br/>
 
</p>
</p>
Line 80: Line 55:
                 - Using ''E. coli'' KRX Genome DNA for PCR <br />
                 - Using ''E. coli'' KRX Genome DNA for PCR <br />
                 - PCR-program: <br />
                 - PCR-program: <br />
 +
 +
<!-- [[Image:Bielefeld-Labjournal-22-05-2013-Tab1.jpg|100x150px|thumb|left|'''Molecular Biotechnology, Master''']]
 +
<img align="right" width="500px" height="400" id="labj001" src="https://static.igem.org/mediawiki/2013/b/ba/Bielefeld-Labjournal-22-05-2013-Tab1.jpg">
 +
<br /><br /> -->
 +
 +
                 Table PCR-program <br />
                 Table PCR-program <br />
                 - PCR products are used for agarose gel electrophoresis <br />
                 - PCR products are used for agarose gel electrophoresis <br />
                 Table gel-program <br />
                 Table gel-program <br />
                 - Agarose gel electrophoresis failed, no bands found <br />
                 - Agarose gel electrophoresis failed, no bands found <br />
-
<br /><br />
 
-
<b>2) Experiment 2</b><br />
 
-
 
-
 
</p>
</p>
</div><!-- /article-text -->
</div><!-- /article-text -->
</div><!-- /article -->
</div><!-- /article -->
-
 
<div class="article" id="w02-16-05">
<div class="article" id="w02-16-05">
Line 147: Line 123:
</html>
</html>
 +
/div>

Revision as of 16:42, 17 June 2013


Week 2, 14.05.2013, Tuesday

Posted on 10/06/2013

1) Starting with experiment Glycerol dehydrogenase
- Preparing overnight culture ''E. coli'' KRX

Week 2, 15.05.2013, Wednesday

Posted on 10/06/2013

1) Glycerol dehydrogenase
- Using overnight culture ''E. coli'' KRX
- Genome Isolation ''E. coli'' KRX using innuPrep Plasmid MiniKit ''JenaAnalytik''
- Using ''E. coli'' KRX Genome DNA for PCR
- PCR-program:
Table PCR-program
- PCR products are used for agarose gel electrophoresis
Table gel-program
- Agarose gel electrophoresis failed, no bands found

Week 2, 16.05.2013, Thursday

Posted on 10/06/2013

1) Glycerol dehydrogenase
- Using'' E. coli'' KRX Genome DNA for PCR
- PCR-program:
Table PCR-components
Table PCR-program
- PCR products are used for agarose gel electrophoresis
Table gel-program
- Agarose gel electrophoresis worked, see image.
Picture Agarosegel glda_1
- Bands are at expected size of 1050 bp


2) Experiment 2

Week 2, 17.05.2013, Friday

Posted on 10/06/2013

1) Glycerol dehydrogenase
- Repeating agarose gel electrophoresis form 23.05.2013 with more sample volume
- Isolation of bands at 1050 bp using innuPrep Gel Extraction Kit ''JenaAnalytik''
- Measurement of DNA-concentration using nanodrop

/div>

Retrieved from "http://2013.igem.org/Template:Team:Bielefeld-Germany/Notebook/Week02"