Team:UT Dallas/week6

From 2013.igem.org

(Difference between revisions)

Latest revision as of 21:03, 23 September 2013

8/11

<a name="h.vvmiq3irzx74"></a>picking colonies from transformation 8/10

<a name="h.olbwgp15g6t9"></a>i5-30-3 → i5-30-8

<a name="h.21q6gds1ehex"></a> i5-30-9

<a name="h.5wc3l82d0jxe"></a>Inoculate from glycerol stock:

<a name="h.4vfyb030afzi"></a>i5-22-5 [Scr]

<a name="h.t60w4w1qz78e"></a>i5-11-5 [pLac-GFP]

<a name="h.sthj625sbh1j"></a>1 → i5-11-5 61.1

<a name="h.9r999ot36cnq"></a>2 → i5-11-5 70.5

<a name="h.32qb54z130re"></a>3 → i5-11-5 58.6

<a name="h.db6xyee79l2m"></a>4 → i5-11-5 53.2

<a name="h.6eijjl1so9ik"></a>5 → i5-11-5 89.5

<a name="h.5dtixuf80mfh"></a>Digestion

<a name="h.8vt5g222tbak"></a>	<a name="h.riiwupqmqcoi"></a>i5-31-1	<a name="h.jsoa1kpxc4vv"></a>i5-31-2	<a name="h.5ruk5040g5t4"></a>i5-31-3	<a name="h.u0empn399lsg"></a>i5-31-4/5
<a name="h.hnfez9nn2o8k"></a>Dna	<a name="h.22wlwhnoii02"></a>ComA (61.1) 32.7	<a name="h.ivc0psafpm3j"></a>ComD (70) 28.5	<a name="h.duls23yq14bd"></a>ComE (58.6) 34.1	<a name="h.pr2gmd48fifd"></a>Dex 30
<a name="h.fcegagyihx5l"></a>water	<a name="h.h60bu4sdaasu"></a>11	<a name="h.nfgfyiefc5up"></a>15	<a name="h.be22s9c5w1j6"></a>10	<a name="h.lecx4rtgqph2"></a>13
<a name="h.sw6apjw78neq"></a>Buffer	<a name="h.mhn3h9cykay"></a>5	<a name="h.3cyhgka656mz"></a>5	<a name="h.7ys3s1iv9zj8"></a>5	<a name="h.4d9gi6dnihxw"></a>5
<a name="h.vxbk98y03h1i"></a>EcoR	<a name="h.vj3akwf13l2h"></a>1	<a name="h.z6mcgyoplpso"></a>0	<a name="h.k133bsa79gki"></a>0	<a name="h.jqxb8sunh8wq"></a>0
<a name="h.kkvqfofng39p"></a>PstI	<a name="h.w6m4vgeqx9h5"></a>0	<a name="h.rd9ij47q4kjx"></a>0	<a name="h.dw7g5lxl7xhz"></a>1	<a name="h.bm7ymci4yk4a"></a>0
<a name="h.5tckkbw5ia12"></a>XbaI	<a name="h.bc4tflx1zoj8"></a>0	<a name="h.scgko0f8q9w7"></a>1	<a name="h.o13kh3yf1av4"></a>1	<a name="h.tbrncavf235g"></a>1
<a name="h.5uedgdiksfu6"></a>SpcI	<a name="h.n2xj9c6hsus1"></a>1	<a name="h.k6jj4syfvv0l"></a>1	<a name="h.swtckdt4933u"></a>0	<a name="h.92mfu5ky8kx8"></a>1

<a name="h.ugixtn8vvmug"></a>i5-31-1 16.5

<a name="h.7wuuci41molh"></a>i5-31-2 10.3

<a name="h.nfcfce2p4r66"></a>i5-31-3 21.0

<a name="h.1lia2mtp2vk9"></a>i5-31-5 12.1

<a name="h.c8mms58srvew"></a>8/12

<a name="h.z60iu2l2d4j"></a>Made glycerol stock of:

<a name="h.si7nyr3khs9e"></a>i5-30-1 [pFru-RBS-GFP]

<a name="h.dfxrx1h9mii1"></a>i5-30-8 [pScr-RBS-GFP]

<a name="h.xja03ev51qni"></a>i5-30-9 [pScr-RBS-GFP]

<a name="h.popdmbz54bhh"></a>i5-30-5 [CP-Cra] (wrong, redid 8/16)

<a name="h.rufx1ux1fjhd"></a>i5-30-7 [CP-Scr]

<a name="h.mw6tnc4qqkw0"></a>Miniprep

<a name="h.si7nyr3khs9e"></a>i5-30-1 → 53.2 [pFru-RBS-GFP]

<a name="h.xmkvqj7cif62"></a>i5-30-8 → 82.0 [pScr-RBS-GFP]

<a name="h.xja03ev51qni"></a>i5-30-9 → 92.3

<a name="h.popdmbz54bhh"></a>i5-30-5 → 78.2

<a name="h.mzxfdsv7dj3u"></a>i5-30-7 → 56.8

<a name="h.bb2xhfn87ro0"></a>Ligation

<a name="h.cfli8oc53zij"></a>	<a name="h.tnl4yi53q6q5"></a>ComA	<a name="h.s672t8di70x2"></a>ComA	<a name="h.xamf4p3sdygb"></a>ComD	<a name="h.xhqfijsu5a81"></a>ComD	<a name="h.iodh2qkxwijg"></a>ComE	<a name="h.q0dxynqaozbt"></a>ComE	<a name="h.1jdaufvz7q36"></a>Dex
<a name="h.wqiqbk8r8gd1"></a>	<a name="h.7qpzbext29gi"></a>3:1	<a name="h.paqkalvi7ivq"></a>0:1	<a name="h.py5u8i4ld045"></a>3:1	<a name="h.98umyyb6hufi"></a>0:1	<a name="h.4zn9fywnaldl"></a>3:1	<a name="h.bowuoxuc9v03"></a>0:1	<a name="h.b0bqolnt4a82"></a>3:1
<a name="h.wqiqbk8r8gd1"></a>Insert	<a name="h.cxlvspvsbbq6"></a>10	<a name="h.cx070p8ko9dq"></a>0	<a name="h.mxqfbwczspx9"></a>6	<a name="h.x6sl1wi2rmrg"></a>0	<a name="h.j32dv9vyty22"></a>8	<a name="h.5y5ferwcv6x6"></a>0	<a name="h.vq89odwvolxf"></a>6.75
<a name="h.ncf56p5a20ln"></a>Vector	<a name="h.x79eciedmwz0"></a>3	<a name="h.ye8nbvfo344y"></a>3	<a name="h.oql65oi32idv"></a>5	<a name="h.l0e0tk65fc7h"></a>5	<a name="h.bqxjrvhy0zyn"></a>2.5	<a name="h.fzqgneabxu1r"></a>2.5	<a name="h.gbqzzss4ic"></a>2
<a name="h.w5xhsa8ln4s"></a>Buffer	<a name="h.o6exq9icnoew"></a>2	<a name="h.8lvpkmnzfss9"></a>2	<a name="h.n1eas5or9s5"></a>2	<a name="h.f1brl88flh57"></a>2	<a name="h.h6f75tjy0f0x"></a>2	<a name="h.cbk9vwmh6eq4"></a>2	<a name="h.5sp623sblow0"></a>2
<a name="h.side06m7hs1z"></a>Ligase	<a name="h.vf7x1ponl3ce"></a>1	<a name="h.57ieivf2yila"></a>1	<a name="h.nlpzer8ivilv"></a>1	<a name="h.9qwnd76rc8te"></a>1	<a name="h.yzv148wv3jtn"></a>1	<a name="h.xq801lqz9aqz"></a>1	<a name="h.t1kzlcyz6tp0"></a>1
<a name="h.eryxzcf41if6"></a>Water	<a name="h.oin1a99r6bel"></a>4	<a name="h.o27rrghxlr2r"></a>14	<a name="h.x8hkiphev4lz"></a>6	<a name="h.iemp08k16607"></a>12	<a name="h.ofephv51plgo"></a>6.5	<a name="h.1bamdauhn2ub"></a>14.5	<a name="h.uk544tp0w364"></a>8.25
<a name="h.w8c2gngxwsbd"></a>Total	<a name="h.4fo03sz9g275"></a>20	<a name="h.dp33idhscmtm"></a>20	<a name="h.sk7070l7diu1"></a>20	<a name="h.ecmr2drb5drx"></a>20	<a name="h.gbkwro9b8qff"></a>20	<a name="h.nyhm7ymibitx"></a>20	<a name="h.9t06fea2a72p"></a>20
<a name="h.557lenwh4acz"></a>	<a name="h.oqmynkqtai6z"></a>Insert: i5-25-3 <a name="h.bnwzdca05ak9"></a>Vector: i5-31-1	<a name="h.oqmynkqtai6z"></a>I: i5-25-3 <a name="h.bnwzdca05ak9"></a>V: i5-31-1	<a name="h.ya3rzcfa7g47"></a>I: i5-25-1 <a name="h.kca81p3gmj9k"></a>V:i5-31-2	<a name="h.ya3rzcfa7g47"></a>I: i5-25-1 <a name="h.kca81p3gmj9k"></a>V:i5-31-2	<a name="h.qauaamczt8gp"></a>I: i5-25-2 <a name="h.8li0bnudb2c4"></a>V: i5-31-3	<a name="h.qauaamczt8gp"></a>I: i5-25-2 <a name="h.8li0bnudb2c4"></a>V: i5-31-3	<a name="h.qauaamczt8gp"></a>I: i5-31-5 <a name="h.8li0bnudb2c4"></a>V: i5-31-2
<a name="h.edscmbgtd87j"></a>	<a name="h.224van5pf5l1"></a>1	<a name="h.382dg1rr800e"></a>2	<a name="h.tid93xvoiipv"></a>3	<a name="h.epzohjyq4x2c"></a>4	<a name="h.n2no183w2p1q"></a>5	<a name="h.q15mpoqb3srg"></a>6	<a name="h.oecqb87ui6x9"></a>7

<a name="h.o9ndf69qcz4j"></a>Transformation

<a name="h.w2q77lo5hvc1"></a>1 →ComA 3:1

<a name="h.720ozsgw6h93"></a>2→ComA 0:1

<a name="h.83lcczouup6g"></a>3→ComD3:1

<a name="h.onc7rknpledz"></a>4→ComD 0:1

<a name="h.4ficwey6o8ig"></a>5→ComE 3:1

<a name="h.6l83pdf75g42"></a>6→ComE 0:1

<a name="h.mq17t4ufsjk"></a>7→Dex → 1 colony

<a name="h.d6xm84vr4x83"></a>8→ + control → 100+ colonies

<a name="h.7qzdi2w8gp73"></a>Test Digestion

<a name="h.ho1jt6iq2h8k"></a>	<a name="h.pkf1jsm450zm"></a>i5-30-1	<a name="h.7el0px6le1a7"></a>i5-30-5	<a name="h.24ib2ir6m2ox"></a>i5-30-7	<a name="h.u9z7nr8b8j0l"></a>i5-30-8	<a name="h.jd3f1732uftp"></a>i5-30-9
<a name="h.hjif9nafr6s5"></a>DNA	<a name="h.b8195jnfhmyt"></a>4	<a name="h.g2vpeq68qpjz"></a>3	<a name="h.tlcg0csj8q9o"></a>4	<a name="h.vtyhzhp29j1s"></a>3	<a name="h.n4fls87mzdah"></a>2
<a name="h.ta72xr1vgm6g"></a>Buffer	<a name="h.6pfp49qxzz2z"></a>2	<a name="h.an7yur76434y"></a>2	<a name="h.bmnsoogx6kls"></a>2	<a name="h.4m15zdyya5rb"></a>2	<a name="h.jg1wwznu9h7i"></a>2
<a name="h.hbxgu9ja18rw"></a>EcoRI	<a name="h.k4z0oqwlyqpk"></a>1	<a name="h.b8wnd3bwqon6"></a>1	<a name="h.548nat6b9w6j"></a>1	<a name="h.8i4o4jvawxr1"></a>1	<a name="h.ogg33qbohcgv"></a>1
<a name="h.5eqsi863qd7m"></a>PstI	<a name="h.9ie5iuta43y9"></a>1	<a name="h.ip7ekaxu5f2r"></a>1	<a name="h.1p411tww0gjc"></a>1	<a name="h.af9dcgo9r8lg"></a>1	<a name="h.8slr5xs1f1d"></a>1
<a name="h.7svg6bh9t6le"></a>Water	<a name="h.5mtz6i934nq2"></a>12	<a name="h.mdut81am6251"></a>13	<a name="h.qfgs3ysmz2q9"></a>12	<a name="h.6r9hcb7b6zdq"></a>13	<a name="h.2pj0e6fg6xdk"></a>14
<a name="h.h4hom3e1ujw3"></a>Total	<a name="h.4706ttpwek2z"></a>20	<a name="h.ml9hn9pp61rx"></a>20	<a name="h.k8veicqw4kv"></a>20	<a name="h.apq0moj322ez"></a>20	<a name="h.rxse6jl2tsge"></a>20

<a name="h.8hp55y1g01wx"></a>8/13

<a name="h.yx6z65elmr5b"></a>Repeat Digestion of CP medium from 8/8

<a name="h.dv5qx0favqqa"></a>	<a name="h.nz82xc7e01g6"></a>i5-25-7
<a name="h.7vv70o4k78pk"></a>DNA	<a name="h.lgu14qf36qfo"></a>20
<a name="h.u1swwb9q8awq"></a>Buffer	<a name="h.5i7qfzvnj3pl"></a>5
<a name="h.qrzhx6mr6wwb"></a>PstI	<a name="h.1088ioza8a33"></a>1
<a name="h.9p92448b6d1q"></a>SpcI	<a name="h.gv2zsmjthhee"></a>1
<a name="h.d132mkn7dsri"></a>Water	<a name="h.qewdkta6kgva"></a>13
<a name="h.huyfpddhtgtt"></a>Total	<a name="h.sfc9bdbspbdo"></a>50

<a name="h.q07fj8aatkj8"></a> → i5-32-1 13.1 ng/ul

<a name="h.h0fq67pbrtou"></a>Ligation

<a name="h.x1caggew13f2"></a>	<a name="h.sdc6rgdv6jlk"></a>Insert: i5-26-8 [Cra]	<a name="h.ow488913hovq"></a>Vector: i5-32-1 [CP]
<a name="h.9hn0spu7m8ih"></a>	<a name="h.s5a78zhhvqcc"></a>3:1	<a name="h.760n4t6ht1gc"></a>0:1
<a name="h.9hn0spu7m8ih"></a>insert	<a name="h.xw2xpb6fx4ou"></a>4.36	<a name="h.olxy8kdv8cnu"></a>0
<a name="h.93kp6ku0iiwh"></a>Vector	<a name="h.rcvg4ygk32zo"></a>3.8	<a name="h.5wmx0igowyu6"></a>3.8
<a name="h.1wsohk9s5r10"></a>Buffer	<a name="h.rfm565dvbc4t"></a>2	<a name="h.rvag37m6puj4"></a>2
<a name="h.pmalz0lmrokt"></a>ligase	<a name="h.pwctjlc04whd"></a>1	<a name="h.f9a1d51d1dmr"></a>1
<a name="h.qa6yiisdvkdk"></a>Water	<a name="h.qk6vu2naq0r6"></a>8.8	<a name="h.yqm7u1tkmdtd"></a>13.2
<a name="h.a3clo8qmlbds"></a>total	<a name="h.z59eeer6r1se"></a>20	<a name="h.5rvon3dlqqxt"></a>20
<a name="h.vn9sjwmsj6xx"></a>	<a name="h.jsrsefbvapq8"></a>1	<a name="h.ag5akp7a4jps"></a>2

<a name="h.ipdc1yd24vxf"></a>	<a name="h.f4wjlhm56vu7"></a>Insert: i5-26-9 [Scr]	<a name="h.f4wjlhm56vu7"></a>Vector: i5-31-1 [CP]
<a name="h.qh1y06b3479c"></a>	<a name="h.48wssfx7r1u7"></a>3:1	<a name="h.48wssfx7r1u7"></a>0:1
<a name="h.9hn0spu7m8ih"></a>insert	<a name="h.b11zavitsha"></a>5.13	<a name="h.b11zavitsha"></a>0
<a name="h.93kp6ku0iiwh"></a>Vector	<a name="h.uq46wo93oxow"></a>3.8	<a name="h.uq46wo93oxow"></a>3.8
<a name="h.1wsohk9s5r10"></a>Buffer	<a name="h.ivm0seypj81u"></a>2	<a name="h.ivm0seypj81u"></a>2
<a name="h.pmalz0lmrokt"></a>ligase	<a name="h.b4bsa7b2vnai"></a>1	<a name="h.b4bsa7b2vnai"></a>1
<a name="h.qa6yiisdvkdk"></a>Water	<a name="h.28sx6m74nth0"></a>8	<a name="h.28sx6m74nth0"></a>13.2
<a name="h.a3clo8qmlbds"></a>total	<a name="h.pqek5guz4619"></a>20	<a name="h.pqek5guz4619"></a>20
<a name="h.ebecajg2zi5f"></a>	<a name="h.bzw6gpt1ozvt"></a>3	<a name="h.bzw6gpt1ozvt"></a>4

<a name="h.q9a8up4ldyhk"></a>Transformation

<a name="h.arfeyr3nrwf1"></a>1 → i5-33-1 → 0 colonies

<a name="h.7j7e2d5zvfnv"></a>2 → i5-33-2 → 0 colonies

<a name="h.tj6n93n3hd83"></a>3 → i5-33-3 → 0 colonies

<a name="h.lj3xj5v1afoj"></a>4→ i5-33-4 → 0 colonies

<a name="h.mqacxtcn19jp"></a>Positive Control (i5-22-2) → 0 colonies

<a name="h.2d1ug8400cez"></a>Retransformation of strong CP fro 8/16 → i5-33-5 → 50 + colonies picked 2 colonies → i5-33-6

<a name="h.m5cstemmlke7"></a> i5-33-7

<a name="h.qw529owinve4"></a>8/14

<a name="h.6s52vkatmbqc"></a>Made Glycerol stock of:

<a name="h.733nppqgji5e"></a>Dex (i5-33-8)

<a name="h.2n5f35kjdexk"></a>Miniprep/ Glycerol stock

<a name="h.56b95g7u2c82"></a>i5-33-8 Dex → 154

<a name="h.l505z58uct98"></a>i5-33-9 Com A-1 → 81.8

<a name="h.woxm5mh4s8yx"></a>i5-33-10 Com D-1 → 70.5

<a name="h.7dy67k57t83u"></a>i5-33-11 Com E-1 → 97.7

<a name="h.j67czes1nje3"></a>i5-33-12 Com A-2 → 77.6

<a name="h.8o82cq131mwq"></a>i5-33-13 Com D-2 → 47.3

<a name="h.nhsd22vcnxzk"></a>i5-33-14 Com E-2 → 127. 5

<a name="h.1vzgbdfgusl"></a>Transformation redo from 8/13

<a name="h.d9xabqe163og"></a>1 → i5-34-1

<a name="h.ajqqufir100x"></a>2 → i5-34-2

<a name="h.mala9ohzl2"></a>3→ i5-34-3 [All colonies counts next page]

<a name="h.5u9mnvrjowzf"></a>4→ i5-34-4

<a name="h.7lp05xi8o5jz"></a>i5-10-1 → positive control

<a name="h.owy03xy20j5h"></a>Test Digestion

<a name="h.lxz4mxrdunih"></a>	<a name="h.v9xpuald20wk"></a>i5-33-8
<a name="h.ci3rk05ry5ry"></a>DNA	<a name="h.x9gcwu5g13mr"></a>2
<a name="h.36xpyj93xpyr"></a>Cutsmart	<a name="h.70xva2p118jk"></a>2
<a name="h.n2y7cvs0db2t"></a>EcoRI	<a name="h.j3mb0eg4shbn"></a>1
<a name="h.qq9rejw4pt8y"></a>PstI	<a name="h.f2q0gtusxx5h"></a>1
<a name="h.ru7ua7fjbnn"></a>Water	<a name="h.7gvq28yviv4r"></a>14
<a name="h.c2bhr457dozj"></a>Total	<a name="h.dzzcigrpxzz9"></a>20

<a name="h.vl55fgfq33t8"></a>Test Digestion

<a name="h.dzj8kdmrw064"></a>	<a name="h.fx2prjlkq76j"></a>i5-33-9	<a name="h.7ht2krvpioy7"></a>i5-33-10	<a name="h.my0t2hlhzat1"></a>i5-33-11	<a name="h.93llo9118vsg"></a>i5-33-12	<a name="h.w0foxt8aex4d"></a>i5-33-13	<a name="h.fd58o8cixmp9"></a>i5-33-14
<a name="h.8t01v6p6g6oc"></a>DNA	<a name="h.n60btcbhfv6l"></a>3	<a name="h.56u2amgcing2"></a>3	<a name="h.uweeq9qxi7z5"></a>3	<a name="h.dhva6bj8wovx"></a>3	<a name="h.i0w5nlihhkey"></a>3	<a name="h.akr7qbkgb7rs"></a>3
<a name="h.1o0slz60o70b"></a>Cutsmart	<a name="h.diuyuwnqt0t0"></a>2	<a name="h.jzdj4uaceps"></a>2	<a name="h.591d5yr5feug"></a>2	<a name="h.yym2agfwpqlm"></a>2	<a name="h.l4b28dk2yyp4"></a>2	<a name="h.hfxdq2uysvok"></a>2
<a name="h.kabmxhl1q7p5"></a>EcoRI	<a name="h.g5a1h31nsq8z"></a>1	<a name="h.t0amuaxmmile"></a>1	<a name="h.fk9ozzb1qlgo"></a>1	<a name="h.fvctb7h3kyk6"></a>1	<a name="h.2b5qhqv78tp1"></a>1	<a name="h.crm4mgms332k"></a>1
<a name="h.kabmxhl1q7p5"></a>PstI	<a name="h.g5a1h31nsq8z"></a>1	<a name="h.t0amuaxmmile"></a>1	<a name="h.fk9ozzb1qlgo"></a>1	<a name="h.fvctb7h3kyk6"></a>1	<a name="h.2b5qhqv78tp1"></a>1	<a name="h.crm4mgms332k"></a>1
<a name="h.5hmye17dbm75"></a>Water	<a name="h.yc85jwx5iojg"></a>13	<a name="h.bdtwpekf68tl"></a>13	<a name="h.z1er9hn2id7m"></a>13	<a name="h.405i5cnqv9jx"></a>13	<a name="h.q31bwpoozg1v"></a>13	<a name="h.bwp6ypriegfa"></a>13
<a name="h.z79365d72210"></a>Total	<a name="h.x1isskclwh35"></a>20	<a name="h.p8rtm5wyi0xu"></a>20	<a name="h.hu038w9qqu68"></a>20	<a name="h.7tr7u75nb19w"></a>20	<a name="h.4reavinajn18"></a>20	<a name="h.f1ewrk9uxa8h"></a>20

<a name="h.goheppc2dx61"></a>Results: inconclusive

<a name="h.si5hs73lcmbi"></a>Repeat Test Digestion

<a name="h.ldjmfp5uvq51"></a>	<a name="h.jj7751sgt03e"></a>i5-33-8
<a name="h.r5ck60k1i6yt"></a>DNA	<a name="h.nit2z0jd4st"></a>2
<a name="h.b079rgaxf3ng"></a>Cutsmart	<a name="h.ufdry88xjj1e"></a>2
<a name="h.gnrr87dmenoa"></a>SpcI	<a name="h.8x3hmh3on29p"></a>1
<a name="h.c8qhrq7nxqpl"></a>XbaI	<a name="h.d7r04bsp65ij"></a>1
<a name="h.754zetd4eibj"></a>Water	<a name="h.ea765ldv4x3v"></a>14
<a name="h.so7akcxwhrno"></a>Total	<a name="h.l0rch97n5392"></a>20

<a name="h.a89iq8grwvpc"></a>Failed

<a name="h.1fl9kmdwiq0s"></a>8/15

<a name="h.9pnxeap1km09"></a>i5-34-1 → 100+ colonies → picked 2 colonies → i5-35-5 [CP(M)-Cra]

<a name="h.h8ns16axtbxy"></a> i5-35-6

<a name="h.rnj1u24eoh1n"></a>i5-34-2 → 0 colonies

<a name="h.hwzvnqy1v49y"></a>i5-34-3 → 50 + colonies → picked 2 colonies → i5-35-3 [CP(M)-Scr]

<a name="h.v0bhzs2t7dg1"></a> i5-35-4

<a name="h.o6k2epid3y1m"></a>i5-34-4 → 2 colonies

<a name="h.lqkxekytz533"></a>+ control → 0 colonies

<a name="h.eb99bx8tsmo"></a>Miniprep And Glycerol Stock made

<a name="h.wgkh4hqdjib9"></a>i5-33-6 → 53.1 ng/ul [CP strong]

<a name="h.2l9z0z37ujxp"></a>i5-33-7 → 58.9 ng/ul [CP strong]

<a name="h.15mzsqz3n0ly"></a>Digestion

<a name="h.bdgsnrytul6"></a>	<a name="h.w9z5jrg1x57h"></a>i5-33-6 (CP strong)
<a name="h.b0c0fmtlkt3f"></a>DNA	<a name="h.91w4r2cdnt0t"></a>30
<a name="h.yig94fpjns1e"></a>NEBuffer	<a name="h.sr5eiumlgmbu"></a>5
<a name="h.9ltstaxfarc7"></a>PstI	<a name="h.s73qz9hfistn"></a>1
<a name="h.u5ilp2lcp5bj"></a>SpeI	<a name="h.wuokcmecm27d"></a>1
<a name="h.ngg479drtbhh"></a>Water	<a name="h.67e3o1g0j5jj"></a>13
<a name="h.gbd3s8pck0vp"></a>Total	<a name="h.3bk31q29c9gh"></a>50

<a name="h.d7zb8v4im8lz"></a> i5-35-1 → 13.9 ng/ul

<a name="h.6fxhopel4kth"></a>	<a name="h.thvwwy6o83wu"></a>i5-10-1 (RBS-GFP)
<a name="h.lri2snfhcour"></a>DNA	<a name="h.hdc1walngwa5"></a>20
<a name="h.js8kzetr99dk"></a>NEBuffer	<a name="h.yw9l8h6wfus8"></a>5
<a name="h.wxr4meh0e6gj"></a>PstI	<a name="h.l6mmbvsgibh8"></a>1
<a name="h.ounargsrp2qk"></a>XbaI	<a name="h.hez37ql5nv4b"></a>1
<a name="h.grnbmt70wwmu"></a>Water	<a name="h.s8i2ruautrt4"></a>23
<a name="h.mhmxk7kbzx91"></a>Total	<a name="h.e8vmy2mwh768"></a>50

<a name="h.e9bxlwesm3h9"></a> i5-35-2 → 17.1 ng/ul

<a name="h.37hiui2t0eqr"></a>PCR

<a name="h.luigpv11kti0"></a>Primers	<a name="h.i83ocmyj89ou"></a>4	<a name="h.onsriba2lcts"></a>10
<a name="h.8ck7zl3t01qq"></a>DNA	<a name="h.vafajfrzu42a"></a>1	<a name="h.d3tpned157iv"></a>1
<a name="h.lojiwgflre5e"></a>F	<a name="h.n76arnyhqnaz"></a>1	<a name="h.nv47yawjriya"></a>1
<a name="h.ohyt97edpj3r"></a>R	<a name="h.l47l0eaq5ibc"></a>1	<a name="h.ufvoe9hrrvr2"></a>1
<a name="h.7q0yr9mz7mxs"></a>Phusion	<a name="h.c0zjhnj8bt96"></a>10	<a name="h.11rmx5egotds"></a>10
<a name="h.vfwxkawdm79z"></a>Water	<a name="h.14kzu2xq6ksa"></a>7	<a name="h.vo12j22ezux6"></a>7
<a name="h.tp7f6moiu9o7"></a>Tota	<a name="h.vigfadqoy52m"></a>20	<a name="h.pj1g9enicib4"></a>20

<a name="h.e6ztamlui3vf"></a>PCR Purification

<a name="h.tzxi0owohar5"></a>1→ 24.4 ng/ul ComA

<a name="h.egr67g98m24o"></a>2→ 43.6 ng/ul Dex

<a name="h.nh4olqlh59mv"></a>Digestion

<a name="h.efof4gy1vxzj"></a>	<a name="h.1z35g8cwhrxr"></a>ComA	<a name="h.2f6rme6cumya"></a>Dex
<a name="h.6w8x1plhemba"></a>DNA	<a name="h.8wptridw7fm"></a>30	<a name="h.v1ye82gh2een"></a>30
<a name="h.quisp77awysu"></a>Buffer	<a name="h.f69r8p31sowc"></a>5	<a name="h.cyk3clpfnky7"></a>5
<a name="h.x0qk4inxyt7n"></a>E	<a name="h.p2ywfsdycopq"></a>1	<a name="h.ndmxu8jdazmm"></a>0
<a name="h.x4glb5qnh54p"></a>X	<a name="h.rpa1pcys26m4"></a>0	<a name="h.8vd9ag7mii8q"></a>1
<a name="h.v19rlpfqg53s"></a>S	<a name="h.2cxf3tm6bc"></a>1	<a name="h.me88l1uu0xpd"></a>1
<a name="h.x7o6sjj1sxsq"></a>Water	<a name="h.fuj809w7vpkf"></a>13	<a name="h.laffqtto3mjy"></a>13
<a name="h.rm77uats1phh"></a>Total	<a name="h.z9ejdad5bi27"></a>50	<a name="h.5qrz284vjn73"></a>50

<a name="h.x1e079w76rvn"></a> i5-36-1 i5-36-2

<a name="h.7ikwtgti8hd5"></a>Ligation

<a name="h.446u184cvrbf"></a>	<a name="h.ja75d7spkaqi"></a>Insert: i5-35-2	<a name="h.k6zu2ge2aals"></a>Vector: i5-26-7
<a name="h.ais0p8pbx53e"></a>	<a name="h.7u0t2140hcem"></a>3:1	<a name="h.61xlumz88osz"></a>0:1
<a name="h.ais0p8pbx53e"></a>Insert	<a name="h.o2vcyjd38d91"></a>3.4	<a name="h.2fthvm98b3is"></a>0
<a name="h.y7nq0i4hddjb"></a>Vector	<a name="h.gr4k6liyrm8f"></a>2	<a name="h.3od12bj6jqka"></a>2
<a name="h.7f9fqw9tfe83"></a>Buffer	<a name="h.78rj1ra7fpk7"></a>2	<a name="h.rzwwuegmcbg3"></a>2
<a name="h.7bcr7zyep0ao"></a>Ligase	<a name="h.26hp2wrkq5us"></a>1	<a name="h.8mjqylqwwqms"></a>1
<a name="h.ou681b2fez89"></a>Water	<a name="h.3m8taamd7nxu"></a>11.6	<a name="h.qpstgtinnx2y"></a>15
<a name="h.gxspftcsh4ih"></a>Total	<a name="h.q9oa3rbhng9g"></a>20	<a name="h.kbwez6myde3b"></a>20
<a name="h.tbjc9f9wah6k"></a>	<a name="h.wawvtmqqmgs4"></a>1	<a name="h.eu425i3oyc1y"></a>2

<a name="h.7r205nsg42zm"></a>	<a name="h.3vi5fw695hr3"></a>Insert: i5-35-2	<a name="h.hwa5u13k1iwv"></a>Vector: i5-32-1
<a name="h.1bvzbhv2uwc9"></a>	<a name="h.am7cktusj87g"></a>3:1	<a name="h.18tkuz7w6jlv"></a>0:1
<a name="h.ais0p8pbx53e"></a>Insert	<a name="h.r26r5wp2h3zy"></a>3.4	<a name="h.av3s0e2o73ag"></a>0
<a name="h.y7nq0i4hddjb"></a>Vector	<a name="h.485wyvn50xr1"></a>3.8	<a name="h.rmtoc1k3iwgv"></a>3.8
<a name="h.7f9fqw9tfe83"></a>Buffer	<a name="h.f2odavphjx44"></a>2	<a name="h.d8fc3j8vu66d"></a>2
<a name="h.7bcr7zyep0ao"></a>Ligase	<a name="h.1jp365pqla93"></a>1	<a name="h.2chpmzvl7gyu"></a>1
<a name="h.ou681b2fez89"></a>Water	<a name="h.e2k95lv8a1o6"></a>9.8	<a name="h.e7b88jjs85vg"></a>13.2
<a name="h.gxspftcsh4ih"></a>Total	<a name="h.wh7bbpdbgw1u"></a>20	<a name="h.xaqtpwlmq1op"></a>20
<a name="h.agdulkfcjm9t"></a>	<a name="h.4n6g30d55mp"></a>3	<a name="h.6zst6h5r0ejn"></a>4

<a name="h.nrlnl2uh09mn"></a>	<a name="h.x49urbrz3psl"></a>Insert: i5-35-2	<a name="h.igj46wb0fylf"></a>Vector: i5-35-1
<a name="h.pi287kbft10o"></a>	<a name="h.1tqf0d29nwo3"></a>3:1	<a name="h.6odrzlxut793"></a>0:1
<a name="h.ais0p8pbx53e"></a>Insert	<a name="h.dvpf17uz7zb4"></a>3.4	<a name="h.1pwj8dtfqvjf"></a>0
<a name="h.y7nq0i4hddjb"></a>Vector	<a name="h.yzrt9o4no5x4"></a>3.8	<a name="h.6j7asswk37rx"></a>3.8
<a name="h.7f9fqw9tfe83"></a>Buffer	<a name="h.yobqbto1ibdx"></a>2	<a name="h.tcji8slkvpx0"></a>2
<a name="h.7bcr7zyep0ao"></a>Ligase	<a name="h.yd2rnm4y8y12"></a>1	<a name="h.pk7pom6d1u2u"></a>1
<a name="h.ou681b2fez89"></a>Water	<a name="h.t0f9rlc21ws"></a>9.8	<a name="h.k9wsifj3hu8y"></a>13.2
<a name="h.gxspftcsh4ih"></a>Total	<a name="h.7pomzfuh0526"></a>20	<a name="h.l7pqn4xi6uh1"></a>20
<a name="h.enkw485jp5hr"></a>	<a name="h.khiijy6wy0l"></a>5	<a name="h.a1d7jjrad43w"></a>6

<a name="h.2blu38ywme91"></a>i5-36-2 → 7.2

<a name="h.efpckwm48ckh"></a>i5-36-1 → 5.5

<a name="h.vsm37xfci8as"></a>Ligation

<a name="h.dxt9q8ssmb2i"></a>	<a name="h.frqh55sr2nnq"></a>insert: i5-36-1	<a name="h.ad8d23ff7t2"></a>Vector: i5-35-1
<a name="h.7k1j0y4fpakg"></a>	<a name="h.vef3x2rqx7dv"></a>3:1	<a name="h.dctwbc6t0l85"></a>0:1
<a name="h.ais0p8pbx53e"></a>Insert	<a name="h.ecvngv16j278"></a>14	<a name="h.um5s8ol7avca"></a>0
<a name="h.y7nq0i4hddjb"></a>Vector	<a name="h.8uecg72bbyyh"></a>2	<a name="h.hvlswlaxagkm"></a>2
<a name="h.7f9fqw9tfe83"></a>Buffer	<a name="h.tarmcxbd8xv"></a>2	<a name="h.g5t1s4dgbdel"></a>2
<a name="h.7bcr7zyep0ao"></a>Ligase	<a name="h.ri21cogkisnp"></a>1	<a name="h.8vvltxqxtq3f"></a>1
<a name="h.ou681b2fez89"></a>Water	<a name="h.85juk2himz11"></a>1	<a name="h.qizhmhurhfil"></a>15
<a name="h.gxspftcsh4ih"></a>Total	<a name="h.588l61v2wpyy"></a>20	<a name="h.ungk98tufb2i"></a>20
<a name="h.gqlikebq8qye"></a>	<a name="h.wv8bg4a2wc3u"></a>1	<a name="h.heh6q5m96vh0"></a>2

<a name="h.537owfrpel2k"></a>	<a name="h.pnjlac6u93rt"></a>Insert: i5-36-2	<a name="h.jlnxtvctx31c"></a>Vector: i5-35-2
<a name="h.c2xoe0c2eh1n"></a>	<a name="h.l9j36qbonyaz"></a>3:1	<a name="h.t5y7pj1zrs9b"></a>0:1
<a name="h.ais0p8pbx53e"></a>Insert	<a name="h.8tisc44fdrm0"></a>11	<a name="h.2t2o6zak6asp"></a>0
<a name="h.y7nq0i4hddjb"></a>Vector	<a name="h.9hbux94l4his"></a>2	<a name="h.u94on5msviiy"></a>2
<a name="h.7f9fqw9tfe83"></a>Buffer	<a name="h.86ufhok2o4bg"></a>2	<a name="h.uxhxzp95ufr9"></a>2
<a name="h.7bcr7zyep0ao"></a>Ligase	<a name="h.rw15wrbffi7n"></a>1	<a name="h.vj2h9qly4owu"></a>1
<a name="h.ou681b2fez89"></a>Water	<a name="h.sqiowcllzkon"></a>4	<a name="h.3kah5vuq02rg"></a>15
<a name="h.gxspftcsh4ih"></a>Total	<a name="h.5gjl5p87tzyb"></a>20	<a name="h.g8zq8upbpurr"></a>20
<a name="h.grj8cm9qh59r"></a>	<a name="h.fj6uecrb8n39"></a>3	<a name="h.j8lc1i1cnvyf"></a>4

<a name="h.wknss8al3bw9"></a>Transformation

<a name="h.i3rp59nff5kj"></a>1 → i5-37-1 (3:1 CP weak-GFP) → 50+ colonies → picked 1 colony → i5-37-1

<a name="h.afr5apqmgh7x"></a>2→ i5-37-2 (0:1) → 0 colonies

<a name="h.6u95ndm7eryf"></a>3→ i5-37-3 (3:1 CP medium-GFP) → 20+ colonies → picked 1 colony → i5-37-3

<a name="h.8tjez9d9wwrq"></a>4→ i5-37-4 (0:1) → 0 colonies

<a name="h.gslcbbx4vbd0"></a>5→ i5-37-5 (3:1 CP strong- GFP) → 10 colonies → picked 1 colony → i5-37-5

<a name="h.nce9o8wtiv9r"></a>6→ i5-37-6 (0:1) → 0 colonies

<a name="h.thw8mfciqpze"></a>+ control with GFP (pLac-GFP) i5-11-5 → i5-37-7 → 200+ colonies → picked 1 colony → i5-37-7

<a name="h.5fzgywok5zsu"></a>+ control without GFP (pLac) i5-10-3 → i5-37-8 → 300+ colonies → picked 1 colony → i5-37-8

<a name="h.p7g4wz3741my"></a>8/16

<a name="h.xenm88qj8tr0"></a>Miniprep and Glycerol Stock made

<a name="h.w7e9rh2vqpkl"></a>i5-35-5 → 102.4 [CP medium -Cra]

<a name="h.zcm807cia938"></a>i5-35-6 → 96.8 [CP medium -Cra]

<a name="h.bx69nd5oum52"></a>i5-35-3 → 174.1 [CP medium -Sur]

<a name="h.hnfr01465w8h"></a>i5-35-4 → 81.9[CP medium -Sur]

<a name="h.5x45icvqjupb"></a>Ligation

<a name="h.480jjn4ddaml"></a>	<a name="h.kgmh70g2y7xo"></a>Insert: i5-36-1	<a name="h.gsx3jhynwuks"></a>Vector: i5-31-1
<a name="h.9nx9wtt0euyr"></a>	<a name="h.vn8opwiae48j"></a>3:1	<a name="h.fphaff3udskg"></a>0:1
<a name="h.tvcfje28ne7o"></a>Vector <a name="h.jjcthnhd65gc"></a>Insert	<a name="h.ue0m7uo64kig"></a>2 <a name="h.6wklvoawyj75"></a>14	<a name="h.4umn0ia7shi"></a>2 <a name="h.lrwxvelm8yr5"></a>0
<a name="h.eks6fegflllt"></a>Buffer	<a name="h.c052kfza5hvj"></a>2	<a name="h.lez442oz67q4"></a>2
<a name="h.igjte5dsmxv"></a>Ligase	<a name="h.84rlo2sfzavb"></a>1	<a name="h.g66311p9ogb8"></a>1
<a name="h.opzit7ttzhgr"></a>Water	<a name="h.sbiii5hnpqbv"></a>1	<a name="h.ykdcw8msca4d"></a>15
<a name="h.ln3bvpo676an"></a>Total	<a name="h.ivtvob47w6ky"></a>20	<a name="h.ebihepncp1fn"></a>20
<a name="h.l8iinr8zi0m2"></a>	<a name="h.uf0qm8vb8pk9"></a>1	<a name="h.b2amah8qzdry"></a>2

<a name="h.x1d44wutcfux"></a>	<a name="h.p490enb55ood"></a>Vector: i5-31-2	<a name="h.f9s6zwrz984e"></a>Insert: i5-36-2
<a name="h.3ydbg5rxcnfw"></a>	<a name="h.iesw723u5fvk"></a>3:1	<a name="h.v57bmrvyii4f"></a>0:1
<a name="h.tvcfje28ne7o"></a>Vector	<a name="h.otvnii1tpwj6"></a>2	<a name="h.70mhhah07aza"></a>2
<a name="h.eks6fegflllt"></a>Insert	<a name="h.mxdk1e2t3mph"></a>11	<a name="h.yrn8y8ux1ost"></a>0
<a name="h.eks6fegflllt"></a>Buffer	<a name="h.nazn83xlqr16"></a>2	<a name="h.1n1692j4zc5f"></a>2
<a name="h.igjte5dsmxv"></a>Ligase	<a name="h.gk7uqqggkn8j"></a>1	<a name="h.vw4m32eo9wed"></a>1
<a name="h.opzit7ttzhgr"></a>Water	<a name="h.qsebwi85qwlx"></a>4	<a name="h.uuu3n62mo9sq"></a>15
<a name="h.ln3bvpo676an"></a>Total	<a name="h.2z45cb58uzve"></a>20	<a name="h.1u63u2j7cbs5"></a>20
<a name="h.weyf48fo89s5"></a>	<a name="h.b393zp9achik"></a>3	<a name="h.x2s8ejdapab3"></a>4

<a name="h.bck05rrifjsn"></a>Digestion

<a name="h.cp3drxo02zlu"></a>	<a name="h.9bgajhef44mo"></a>i5-35-5	<a name="h.ilytc521q7z8"></a>i5-35-3
<a name="h.ftgy4652e3jj"></a>DNA	<a name="h.4zwquazf9r00"></a>20	<a name="h.5vkb6663ecbc"></a>20
<a name="h.cw8ungggm5i2"></a>NEBuffer	<a name="h.n97w1d3ne244"></a>5	<a name="h.6r3hr3ocjrlh"></a>5
<a name="h.5cwj9i1t3fn8"></a>PstI	<a name="h.cn1rhr6fozee"></a>1	<a name="h.idpxjh190o22"></a>1
<a name="h.drgnwxr8oakf"></a>SpeI	<a name="h.8tw8lhpig6pm"></a>1	<a name="h.p7gjrzcrha61"></a>1
<a name="h.t579oqr0nmx4"></a>Water	<a name="h.gelxflpj4r9o"></a>13	<a name="h.rhr78tydnl0v"></a>13
<a name="h.16nz6uq6ddrr"></a>Total	<a name="h.622yhn26s54o"></a>50	<a name="h.ywwgyfb4gcot"></a>50
<a name="h.5pgdl8fqw7i"></a>	<a name="h.5httc9x9d6rz"></a>i5-38-5	<a name="h.ybu75mfzznxf"></a>i5-38-6

<a name="h.yexdchbtvwmh"></a>	<a name="h.a0ub1rdwnu0m"></a>i5-30-1	<a name="h.oorpyfrunmgr"></a>i5-30-8
<a name="h.ftgy4652e3jj"></a>DNA	<a name="h.xdo1mb5698j4"></a>20	<a name="h.rwx9ye92aef"></a>20
<a name="h.cw8ungggm5i2"></a>NEBuffer	<a name="h.8uwpbjiox103"></a>5	<a name="h.y6ti414jt9dj"></a>5
<a name="h.5cwj9i1t3fn8"></a>PstI	<a name="h.dtp9b4osul7y"></a>1	<a name="h.b0sf5zoszj33"></a>1
<a name="h.drgnwxr8oakf"></a>XbaI	<a name="h.1b0mlm3ivl2e"></a>1	<a name="h.tjl2sgugzhus"></a>1
<a name="h.t579oqr0nmx4"></a>Water	<a name="h.lsjz7a77eu04"></a>13	<a name="h.qba6p47y043k"></a>13
<a name="h.16nz6uq6ddrr"></a>Total	<a name="h.vpq2fyh1dpf4"></a>50	<a name="h.y1b7c1a8jlet"></a>50
<a name="h.m8tt9g2ks5iq"></a>	<a name="h.nog0hulwra5m"></a>i5-38-7	<a name="h.bqlxrvbujnv4"></a>i5-38-8

<a name="h.1cho3s998m0m"></a>Transformation

<a name="h.42b7i439vc6k"></a>i5-36-1 3:1 → i5-38-1 → 30 colonies → picked 2 colonies → i5-38-9

<a name="h.a6yy48ute5r8"></a> i5-38-10

<a name="h.xsfqvvxh8333"></a>i5-36-1 0:1 → i5-38-2 → 8 colonies

<a name="h.k28xj92cimb8"></a>i5-36-2 3:1 → i5-38-3 → 0 colonies

<a name="h.7fp5osalqr18"></a>i5-36-2 0:1 → i5-38-4 → 1 colonies

<a name="h.iwcul570p9na"></a>Control → i5-38-11

<a name="h.c4b87hf8e4u2"></a>Digestion

<a name="h.chyifkx13l2y"></a>Digested parts

<a name="h.e5i57g1il10m"></a>i5-38-5 (Backbone- CP-Cra) → 19.0

<a name="h.wukqvlly8r0c"></a>i5-38-6 (Backbone- CP-Scr) → 43.2

<a name="h.abr9bnvsvkgm"></a>i5-38-7 (pFru-RBS-GFP) → 11.3

<a name="h.daq45zhhkkz5"></a>i5-38-8 (pScr-RBS-GFP) → 4.9

<a name="h.n2fbxy6ebygf"></a>Ligation

<a name="h.saafwxx92ife"></a>	<a name="h.zngy8nh8t0c"></a>Insert: i5-38-7 (pFru-RBS-GFP)	<a name="h.b2s1h0zgot4e"></a>Vector: i5-38-5 (Backbone-CP-Cra)
<a name="h.larjp1pa1p5r"></a>	<a name="h.8n8dcb1c3pya"></a>3:1	<a name="h.ila13r6f7fe0"></a>0:1
<a name="h.d6f2eeurl6w5"></a>Insert	<a name="h.p9twdfyqnnr"></a>4.4	<a name="h.cs1kt2qhih0"></a>0
<a name="h.1fnje6wmn90k"></a>Vector	<a name="h.vjl1f549c3b6"></a>2.6	<a name="h.3yy0tohq2vkg"></a>2.6
<a name="h.o986boq4pg56"></a>Buffer	<a name="h.2uxkdcxph2iu"></a>2	<a name="h.xa7jauc842h1"></a>2
<a name="h.a5dgfsjas4of"></a>Ligase	<a name="h.lj6ftrh52v0l"></a>1	<a name="h.bwajompcq8rn"></a>1
<a name="h.ez3nqb2i6p2c"></a>Water	<a name="h.yvi76vssdmla"></a>10	<a name="h.4h7kxerze1n8"></a>14.4
<a name="h.r8lccqmt9kep"></a>Total	<a name="h.fvmmsj4rq1e9"></a>20	<a name="h.s2ijm1ptkp4j"></a>20
<a name="h.ro5oph2r63qm"></a>	<a name="h.86ekxis5zcy4"></a>1	<a name="h.oi3nbmngom5u"></a>2

<a name="h.luz9d9u2roo4"></a>	<a name="h.1mx048cy7a66"></a>Insert: i5-38-8 (pScr-RBS-GFP)	<a name="h.d38pefz839xv"></a>Vector: i5-38-6 (Backbone-CP-SCr)
<a name="h.pz8gd921dj79"></a>	<a name="h.1b0li5mc4w8g"></a>3:1	<a name="h.gu6vcuegktuy"></a>0:1
<a name="h.d6f2eeurl6w5"></a>Insert	<a name="h.mvxyrz3lem07"></a>9.2	<a name="h.xa9jn6dvh9hn"></a>0
<a name="h.1fnje6wmn90k"></a>Vector	<a name="h.hop631q5blju"></a>1.2	<a name="h.sxfooeqscf76"></a>1.2
<a name="h.o986boq4pg56"></a>Buffer	<a name="h.bnpzz3i5811g"></a>2	<a name="h.mgripwfa4eml"></a>2
<a name="h.a5dgfsjas4of"></a>Ligase	<a name="h.lhmoobl0yz5y"></a>1	<a name="h.p261b7hwttvu"></a>1
<a name="h.ez3nqb2i6p2c"></a>Water	<a name="h.om2zvwusi0oj"></a>6.6	<a name="h.bfarl4enbmbv"></a>15.8
<a name="h.r8lccqmt9kep"></a>Total	<a name="h.ub6tthpcn1n9"></a>20	<a name="h.ksg5qvl5c9s7"></a>20
<a name="h.z1but4l66e9c"></a>	<a name="h.c2v6kg515cm9"></a>3	<a name="h.67vm5tw4jb6l"></a>4

<a name="h.308r4he0txvj"></a>1→ i5-39-1 (3:1 CP-Cra-pFri-RBS-GFP) → 100+ colonies

<a name="h.ghls9styo5zv"></a>2→ i5-39-2 (0:1)--> 300+ colonies

<a name="h.3ahcudohx7yw"></a>3→ i5-39-3 (3:! CP-Scr-pScr-RBS-GFP) → 200+ colonies

<a name="h.6vruk3cepto2"></a>4→ i5-39-4 (0:1) → 200+ colonies

<a name="h.ue2ilzk7m62u"></a>i5-11-5 → positive control (pLac-GFP) → TNTC

<a name="h.a33r7clke9l9"></a>8/16

<a name="h.n3hd43aw1rcu"></a>Made glycerol stock

<a name="h.3539oxtcjglw"></a>i5-37-1 (CP weak -GFP)

<a name="h.6kzdjah0vru8"></a>i5-37-3 (CP medium-GFP)

<a name="h.nsiyrfar8tjx"></a>i5-37-5 (CP strong-GFP)

Revision as of 20:56, 23 September 2013 (view source) Alecspin (Talk \| contribs) (Created page with "<p class="c2"><span>8/4</span></p><p class="c1"><a name="h.sgg25dthi5y5"></a></p><a href="#" name="b65b6d34fbf5c5fb5aaaa4002212f34d7190e2a0"></a><a href="#" name="19"></a><table ...") ← Older edit		Latest revision as of 21:03, 23 September 2013 (view source) Alecspin (Talk \| contribs)
Line 1:		Line 1:
-	<p class="c2"><span>8/4</span></p><p class="c1"><a name="h.sgg25dthi5y5"></a></p><a href="#" name="b65b6d34fbf5c5fb5aaaa4002212f34d7190e2a0"></a><a href="#" name="19"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.a5jijuql1rvr"></a><span>Digestion</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ezp4ebrmrt24"></a><span>i5-19-9</span></p></td><td class="c13"><p class="c4 c2"><a name="h.iabe8cgq9hmu"></a><span>i5-19-10</span></p></td><td class="c13"><p class="c4 c2"><a name="h.7yg7kzgsqtkh"></a><span>i5-19-11</span></p></td><td class="c13"><p class="c4 c2"><a name="h.3q6b1wcbissm"></a><span>i5-19-12</span></p></td><td class="c13"><p class="c4 c2"><a name="h.k8ue8imb8tnj"></a><span>i5-19-13</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ma78axahim7x"></a><span>i5-19-14</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.61t44pohvsaq"></a><span>DNA</span></p></td><td class="c13"><p class="c4 c2"><a name="h.x56fdk4v3ovw"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.2hvapkiw4m87"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.6f886gfut9m0"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.h8oscj46xqwu"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.kbbpg5ihzoll"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.my8jpea79q93"></a><span>2</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.w9esfgelg75h"></a><span>Buffer</span></p></td><td class="c13"><p class="c4 c2"><a name="h.cfww47qpd4af"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.qdl9k4lnp4su"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.abiben5rcnr3"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.3qcfojjf5lcr"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.w7s2l12kuc50"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.dzu83mp3t41"></a><span>1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.af2xg2y7avl3"></a><span>EcoR</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ni5uft1ulrul"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.1wo6olhwpjw2"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.wchoy4dr7x4g"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.tbg88l1b6cy2"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.c5y5gdkqyue2"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.1am4iyn15gpa"></a><span>1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.4tfklkehn3i5"></a><span>PstI</span></p></td><td class="c13"><p class="c4 c2"><a name="h.y5p3h0ubvj0y"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.6i9m52boa53p"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.13m0x4keo2uy"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.yfvo3v5lne2u"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.l32zngxtccv"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.xiw4zxi2jl9m"></a><span>1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.zi71ebctrsd"></a><span>Water</span></p></td><td class="c13"><p class="c4 c2"><a name="h.d0clbz3fd71r"></a><span>5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ryzpdn4j22ul"></a><span>5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.gmgufotxnxut"></a><span>5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.kp5bilx6wvit"></a><span>5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.3vycwm7wlzip"></a><span>5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.6c3d5q4bnsd1"></a><span>5</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.pfexr4rksv9i"></a><span>Total</span></p></td><td class="c13"><p class="c4 c2"><a name="h.xs9j65pjtnxv"></a><span>10</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ayq5fs1mxbjc"></a><span>10</span></p></td><td class="c13"><p class="c4 c2"><a name="h.pk8u9by8feav"></a><span>10</span></p></td><td class="c13"><p class="c4 c2"><a name="h.aou9icqk61cv"></a><span>10</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ez4mpw3kmvv0"></a><span>10</span></p></td><td class="c13"><p class="c4 c2"><a name="h.vfyzzhbb5ljt"></a><span>10</span></p></td></tr></tbody></table><p class="c1"><a name="h.2yrznbpl1lfj"></a></p><p class="c2"><a name="h.o6k1q9vzjd7a"></a><span>8/5 </span></p><p class="c2"><a name="h.2jmyh9gl83yq"></a><span>Digestion</span></p><p class="c1"><a name="h.ii8nthcymtpb"></a></p><a href="#" name="5d3e127f652e674bf47a39df42206f4c48805de0"></a><a href="#" name="20"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c13"><p class="c1 c4"><a name="h.a6dix5iagyun"></a></p></td><td class="c13"><p class="c4 c2"><a name="h.n9qgtg7uhhub"></a><span>i5-19-1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.x3s5avd5kmni"></a><span>i5-19-3</span></p></td><td class="c13"><p class="c4 c2"><a name="h.4gq7ivf6m0v5"></a><span>i5-19-5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.73crz0713h49"></a><span>i5-19-7</span></p></td><td class="c13"><p class="c4 c2"><a name="h.6nra6sudasj9"></a><span>i5-19-11</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ao2ac858ohn1"></a><span>i5-19-13</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.ooejk4dkc05a"></a><span>DNA</span></p></td><td class="c13"><p class="c4 c2"><a name="h.wjvlwqn0s8dc"></a><span>5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.eeg76xr9b7fv"></a><span>12.5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.pfzeo3ypcsto"></a><span>7.1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.qaoefpqrj4d7"></a><span>4</span></p></td><td class="c13"><p class="c4 c2"><a name="h.qu57tb5cdrok"></a><span>5.8</span></p></td><td class="c13"><p class="c4 c2"><a name="h.hgttgxoa6ab0"></a><span>5</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.e2razpc3mzde"></a><span>Buffer</span></p></td><td class="c13"><p class="c4 c2"><a name="h.f6uav9yzdyuy"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.4aqyawqr5qv3"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.b6rdcctzd01d"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.g5zt6eo2s21p"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.9skiq0maxx9b"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.qk65utt0ptcm"></a><span>2</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.4bnct5urijfx"></a><span>EcoR</span></p></td><td class="c13"><p class="c4 c2"><a name="h.33oeogcp86tv"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.jbie2odvtq7x"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.xblh5q1t1us0"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.hkms45pn6iwi"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.3j60bs4b7afo"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.l9onmyyqpe7z"></a><span>1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.401yo1d7kmd3"></a><span>PstI</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ifx7lnwalcxl"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.bw9e2h26z0uf"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.dnizgujui6pw"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.x5v4qjvauhyc"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.stev3lmw8nlg"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.tt6o8acvxkx"></a><span>1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.x6hciqcwfojq"></a><span>Water</span></p></td><td class="c13"><p class="c4 c2"><a name="h.9ht31r5zgfvd"></a><span>11</span></p></td><td class="c13"><p class="c4 c2"><a name="h.gv4kxpd8xtgw"></a><span>3.5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.yp8bd29zeip0"></a><span>8.9</span></p></td><td class="c13"><p class="c4 c2"><a name="h.n372tyu3vdn2"></a><span>12</span></p></td><td class="c13"><p class="c4 c2"><a name="h.or1waswqa7s0"></a><span>10.2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.26z1dl5b9lkj"></a><span>11</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.eho85b950qz4"></a><span>Total</span></p></td><td class="c13"><p class="c4 c2"><a name="h.yfnq9ah2mle0"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.wqspntd4kqe0"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.2jm6j51z1lg"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.9q55arl12i8e"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.wdg4qulbn5ru"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.bgbd4xfuntjm"></a><span>20</span></p></td></tr></tbody></table><p class="c1"><a name="h.5zjr9xt41w5m"></a></p><a href="#" name="edfa04fda6ad415d6e2fba616fd8950368ba86e6"></a><a href="#" name="21"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c89"><p class="c1 c4"><a name="h.a6dix5iagyun"></a></p></td><td class="c30"><p class="c4 c2"><a name="h.n9qgtg7uhhub"></a><span>i5-19-1</span></p></td></tr><tr class="c8"><td class="c89"><p class="c4 c2"><a name="h.ooejk4dkc05a"></a><span>DNA</span></p></td><td class="c30"><p class="c4 c2"><a name="h.wjvlwqn0s8dc"></a><span>5</span></p></td></tr><tr class="c8"><td class="c89"><p class="c4 c2"><a name="h.e2razpc3mzde"></a><span>Buffer</span></p></td><td class="c30"><p class="c4 c2"><a name="h.f6uav9yzdyuy"></a><span>2</span></p></td></tr><tr class="c8"><td class="c89"><p class="c4 c2"><a name="h.4bnct5urijfx"></a><span>EcoR</span></p></td><td class="c30"><p class="c4 c2"><a name="h.33oeogcp86tv"></a><span>1</span></p></td></tr><tr class="c8"><td class="c89"><p class="c4 c2"><a name="h.401yo1d7kmd3"></a><span>PstI</span></p></td><td class="c30"><p class="c4 c2"><a name="h.ifx7lnwalcxl"></a><span>1</span></p></td></tr><tr class="c8"><td class="c89"><p class="c4 c2"><a name="h.x6hciqcwfojq"></a><span>Water</span></p></td><td class="c30"><p class="c4 c2"><a name="h.9ht31r5zgfvd"></a><span>11</span></p></td></tr><tr class="c8"><td class="c89"><p class="c4 c2"><a name="h.eho85b950qz4"></a><span>Total</span></p></td><td class="c30"><p class="c4 c2"><a name="h.yfnq9ah2mle0"></a><span>20</span></p></td></tr></tbody></table><p class="c1"><a name="h.9s6iusvs6p02"></a></p><p class="c2"><a name="h.31al3sts3g4k"></a><span>Picked colonies from transformation of 8/2 and 8/1</span></p><p class="c1"><a name="h.6koh1p1nz9b7"></a></p><p class="c2"><a name="h.fltmgetfd1cq"></a><span>Colonies from 8/1</span></p><p class="c2"><a name="h.ap81cglnf23i"></a><span>1→ i5-22-1         Com B</span></p><p class="c2"><a name="h.17hi9mgj55xx"></a><span>4→ i5-22-2        pScr        Made glycerol stock for all 6</span></p><p class="c2"><a name="h.l5rilafwggo2"></a><span>5→ i5-22-3         Cra</span></p><p class="c2"><a name="h.4rt4e9ch9m8g"></a><span>7→ i5-22-4         Com C</span></p><p class="c1"><a name="h.ibu662kjaieq"></a></p><p class="c2"><a name="h.mzbnml4va3h9"></a><span>Colonies from 8/2 </span></p><p class="c2"><a name="h.bt2c6b5iji0t"></a><span>3 → i5-22-5        SCr</span></p><p class="c2"><a name="h.am7rhjrwfeew"></a><span>6→ i5-22-5        pFru</span></p><p class="c1"><a name="h.3osb89fv4fo1"></a></p><p class="c2"><a name="h.n85mmvxms0xm"></a><span>8/6 </span></p><p class="c2"><a name="h.uhbw6a447c9c"></a><span>Miniprep        Concentrations (ng/ul)</span></p><p class="c2"><a name="h.u1c3fa9lxf43"></a><span>i5-22-1                67.9        Com B</span></p><p class="c2"><a name="h.2s16kmp8fwjo"></a><span>i5-22-2                53.0        pScr</span></p><p class="c2"><a name="h.x2riupq5fzvg"></a><span>i5-22-3                90.9        Cra</span></p><p class="c2"><a name="h.3lehouxtgx4g"></a><span>i5-22-4                68.1         Com C</span></p><p class="c2"><a name="h.ghmduw89ynii"></a><span>i5-22-5                101.8        Scr</span></p><p class="c2"><a name="h.lj0ad7dpkd0a"></a><span>i5-22-6                185.5        pFru</span></p><p class="c1"><a name="h.tamily566yxl"></a></p><p class="c2"><a name="h.2pzbef12bxzr"></a><span>Test Digestion</span></p><p class="c1"><a name="h.qnwry7b6xsb"></a></p><a href="#" name="810c30ac7ea7af7f46d0637a23252142e0dd376c"></a><a href="#" name="22"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c13"><p class="c1 c4"><a name="h.yb5evczhpuom"></a></p></td><td class="c13"><p class="c4 c2"><a name="h.38w6pbrpqzdp"></a><span>i5-22-1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.zgn0l6d6vb5t"></a><span>i5-22-2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.r0yng41r0sic"></a><span>i5-22-3</span></p></td><td class="c13"><p class="c4 c2"><a name="h.p87wenjmpjwd"></a><span>i5-22-4</span></p></td><td class="c13"><p class="c4 c2"><a name="h.gkiou64ws2yz"></a><span>i5-22-5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.v2g12flru3jf"></a><span>i5-22-6</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.va3lacp26cyj"></a><span>DNA</span></p></td><td class="c13"><p class="c4 c2"><a name="h.yg4cfww47xgo"></a><span>3</span></p></td><td class="c13"><p class="c4 c2"><a name="h.9tdm4gqjkkrq"></a><span>4</span></p></td><td class="c13"><p class="c4 c2"><a name="h.442vr9tpdgd"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.o8mgufjbn5t0"></a><span>2.8</span></p></td><td class="c13"><p class="c4 c2"><a name="h.dt9091mp8qd3"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ulcrx6jqi4nf"></a><span>1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.kcdjdapobt6l"></a><span>Cutsmart</span></p></td><td class="c13"><p class="c4 c2"><a name="h.elf3qphugt90"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.3aenyfvkyno8"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ngwgkceddqu3"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.vls4ubjauel4"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.j3boni8avurg"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.sofpjesu1oxp"></a><span>2</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.5ybhn2jvcnur"></a><span>EcoR-HF</span></p></td><td class="c13"><p class="c4 c2"><a name="h.85q2gdi4ggxr"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.twfsbe5gdm9"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.c1ss864a3wyk"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.nmpu7yj3iswf"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.h4x5nkmbjj16"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.8wblq31iu8g7"></a><span>1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.diecaj2350pk"></a><span>Spe I</span></p></td><td class="c13"><p class="c4 c2"><a name="h.431hxums39ls"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ub0o1kbmag9s"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.bt4rjiep29yt"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.z7prz5q61f4i"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.5zwv242rkfvi"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.5bb79vg9hwzd"></a><span>1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.6gpw0rpjdfqg"></a><span>Water</span></p></td><td class="c13"><p class="c4 c2"><a name="h.tgamgczid50z"></a><span>13</span></p></td><td class="c13"><p class="c4 c2"><a name="h.g66iquhf4yb2"></a><span>12</span></p></td><td class="c13"><p class="c4 c2"><a name="h.daevk46emsi9"></a><span>14</span></p></td><td class="c13"><p class="c4 c2"><a name="h.5ctf9kjxkhwb"></a><span>13.2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.chhppzctwrg1"></a><span>14</span></p></td><td class="c13"><p class="c4 c2"><a name="h.plsnqnkfaspd"></a><span>15</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.2r87y2owrqhc"></a><span>Total</span></p></td><td class="c13"><p class="c4 c2"><a name="h.cmo9e7puvjlm"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ag8h3mnqd2l7"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.p4uocxac3rwe"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.izmeh02q5kv7"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.k5xtypjrck2a"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.uyb7c1nwgy0u"></a><span>20</span></p></td></tr></tbody></table><p class="c1"><a name="h.tgwmyrwiukz7"></a></p><p class="c2"><a name="h.e4kpgf1mueqv"></a><span>Digestion of backbone (pLac-GFP i5-11-5)</span></p><p class="c1"><a name="h.stcgnprpfqqm"></a></p><a href="#" name="2ba8e1570a206c8c0cd15a29df10cc1f3dacbf85"></a><a href="#" name="23"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c44"><p class="c1 c4"><a name="h.d25ftzkasjyj"></a></p></td><td class="c16"><p class="c4 c2"><a name="h.vwcs3u4w170g"></a><span>Com C (54.1)</span></p></td><td class="c16"><p class="c4 c2"><a name="h.c6xlvkt0pe77"></a><span>Com D (46.4)</span></p></td><td class="c28"><p class="c4 c2"><a name="h.o4fv70lu3990"></a><span>ComE (84.3)</span></p></td></tr><tr class="c8"><td class="c44"><p class="c4 c2"><a name="h.44ngp9sc0gsx"></a><span>DNA</span></p></td><td class="c16"><p class="c4 c2"><a name="h.roe6ssa2ml6y"></a><span>40</span></p></td><td class="c16"><p class="c4 c2"><a name="h.fdpi22eu3m0i"></a><span>20</span></p></td><td class="c28"><p class="c4 c2"><a name="h.vg5gqoz8kjjv"></a><span>25</span></p></td></tr><tr class="c8"><td class="c44"><p class="c4 c2"><a name="h.q0d68ztwehmr"></a><span>Buffer</span></p></td><td class="c16"><p class="c4 c2"><a name="h.v3t9mcfcbb2p"></a><span>5</span></p></td><td class="c16"><p class="c4 c2"><a name="h.dyjmwex93rs8"></a><span>5</span></p></td><td class="c28"><p class="c4 c2"><a name="h.9okzlob9xvmu"></a><span>5</span></p></td></tr><tr class="c8"><td class="c44"><p class="c4 c2"><a name="h.nca0yzv523b"></a><span>Eco R</span></p></td><td class="c16"><p class="c4 c2"><a name="h.vh9t3cy5bmo6"></a><span>1</span></p></td><td class="c16"><p class="c4 c2"><a name="h.b6x14t79v1k4"></a><span>0</span></p></td><td class="c28"><p class="c4 c2"><a name="h.np51w6d9b29k"></a><span>0</span></p></td></tr><tr class="c8"><td class="c44"><p class="c4 c2"><a name="h.5ago9jf3relr"></a><span>PstI</span></p></td><td class="c16"><p class="c4 c2"><a name="h.gqfk1jvl3ux7"></a><span>0</span></p></td><td class="c16"><p class="c4 c2"><a name="h.8d23ozny1z0m"></a><span>0</span></p></td><td class="c28"><p class="c4 c2"><a name="h.85nkqupm56uz"></a><span>1</span></p></td></tr><tr class="c8"><td class="c44"><p class="c4 c2"><a name="h.tqxwofs4qxbc"></a><span>XbaI</span></p></td><td class="c16"><p class="c4 c2"><a name="h.dwkx341va5rr"></a><span>0</span></p></td><td class="c16"><p class="c4 c2"><a name="h.b43lcolhjx74"></a><span>1</span></p></td><td class="c28"><p class="c4 c2"><a name="h.5235tbrpkcom"></a><span>1</span></p></td></tr><tr class="c8"><td class="c44"><p class="c4 c2"><a name="h.pznia856efr"></a><span>SpcI</span></p></td><td class="c16"><p class="c4 c2"><a name="h.hf4euicphgpb"></a><span>1</span></p></td><td class="c16"><p class="c4 c2"><a name="h.yu17ovg1nn2k"></a><span>1</span></p></td><td class="c28"><p class="c4 c2"><a name="h.sip7erk41zf6"></a><span>0</span></p></td></tr><tr class="c8"><td class="c44"><p class="c4 c2"><a name="h.wnwxgm43end6"></a><span>Water</span></p></td><td class="c16"><p class="c4 c2"><a name="h.zdgligi8l7q8"></a><span>3</span></p></td><td class="c16"><p class="c4 c2"><a name="h.cukagcofwo2c"></a><span>23</span></p></td><td class="c28"><p class="c4 c2"><a name="h.xn42hpzcmfmz"></a><span>18</span></p></td></tr><tr class="c8"><td class="c44"><p class="c4 c2"><a name="h.pvrd163duddt"></a><span>Total</span></p></td><td class="c16"><p class="c4 c2"><a name="h.i9ljeyx3xeak"></a><span>50</span></p></td><td class="c16"><p class="c4 c2"><a name="h.enwfnqcs2nbq"></a><span>50</span></p></td><td class="c28"><p class="c4 c2"><a name="h.j2h3yznwe43u"></a><span>50</span></p></td></tr></tbody></table><p class="c1"><a name="h.s31a92d1wvih"></a></p><p class="c2"><a name="h.y9wahg3dflx7"></a><span>Digestion of insert</span></p><p class="c1"><a name="h.ai7xrolf51jo"></a></p><a href="#" name="4cdf434ed1f855c880097220a3ba4c66edfc753a"></a><a href="#" name="24"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c52"><p class="c1 c4"><a name="h.uh8rvi2dflk4"></a></p></td><td class="c39"><p class="c4 c2"><a name="h.tf68l84o8uvo"></a><span>Com A</span></p></td><td class="c63"><p class="c4 c2"><a name="h.nz13dv1nbx1n"></a><span>Com D</span></p></td><td class="c90"><p class="c4 c2"><a name="h.bnddbpky3te0"></a><span>Com E</span></p></td></tr><tr class="c8"><td class="c52"><p class="c4 c2"><a name="h.44ngp9sc0gsx"></a><span>DNA</span></p></td><td class="c39"><p class="c4 c2"><a name="h.25pbpv9anmf5"></a><span>30</span></p></td><td class="c63"><p class="c4 c2"><a name="h.z0agsj6mnyww"></a><span>30</span></p></td><td class="c90"><p class="c4 c2"><a name="h.ssbk3twkauok"></a><span>30</span></p></td></tr><tr class="c8"><td class="c52"><p class="c4 c2"><a name="h.q0d68ztwehmr"></a><span>Buffer</span></p></td><td class="c39"><p class="c4 c2"><a name="h.z5j53jq0ix0"></a><span>5</span></p></td><td class="c63"><p class="c4 c2"><a name="h.gbddpo6rdg2a"></a><span>5</span></p></td><td class="c90"><p class="c4 c2"><a name="h.y981msirfbq4"></a><span>5</span></p></td></tr><tr class="c8"><td class="c52"><p class="c4 c2"><a name="h.nca0yzv523b"></a><span>Eco R</span></p></td><td class="c39"><p class="c4 c2"><a name="h.asxo1syv4hg1"></a><span>1</span></p></td><td class="c63"><p class="c4 c2"><a name="h.x71ph9ps5jlo"></a><span>0</span></p></td><td class="c90"><p class="c4 c2"><a name="h.caep0rvgrvh0"></a><span>0</span></p></td></tr><tr class="c8"><td class="c52"><p class="c4 c2"><a name="h.5ago9jf3relr"></a><span>PstI</span></p></td><td class="c39"><p class="c4 c2"><a name="h.xxnp9vpfcyzi"></a><span>0</span></p></td><td class="c63"><p class="c4 c2"><a name="h.uh2ny991oqia"></a><span>0</span></p></td><td class="c90"><p class="c4 c2"><a name="h.ju4aq5ij8k44"></a><span>1</span></p></td></tr><tr class="c8"><td class="c52"><p class="c4 c2"><a name="h.tqxwofs4qxbc"></a><span>XbaI</span></p></td><td class="c39"><p class="c4 c2"><a name="h.qihmsot6pl2a"></a><span>0</span></p></td><td class="c63"><p class="c4 c2"><a name="h.xtzo6ikfe7fq"></a><span>1</span></p></td><td class="c90"><p class="c4 c2"><a name="h.fje9ztr5fsb6"></a><span>1</span></p></td></tr><tr class="c8"><td class="c52"><p class="c4 c2"><a name="h.pznia856efr"></a><span>SpcI</span></p></td><td class="c39"><p class="c4 c2"><a name="h.nxfafdazif22"></a><span>1</span></p></td><td class="c63"><p class="c4 c2"><a name="h.la1z3c7emzmo"></a><span>1</span></p></td><td class="c90"><p class="c4 c2"><a name="h.qceayaqmo7q1"></a><span>0</span></p></td></tr><tr class="c8"><td class="c52"><p class="c4 c2"><a name="h.wnwxgm43end6"></a><span>Water</span></p></td><td class="c39"><p class="c4 c2"><a name="h.8ql6x1j0gvnk"></a><span>13</span></p></td><td class="c63"><p class="c4 c2"><a name="h.t9bpwap1kgw7"></a><span>13</span></p></td><td class="c90"><p class="c4 c2"><a name="h.7w9zite8hug9"></a><span>13</span></p></td></tr><tr class="c8"><td class="c52"><p class="c4 c2"><a name="h.pvrd163duddt"></a><span>Total</span></p></td><td class="c39"><p class="c4 c2"><a name="h.659ckxaagez4"></a><span>50</span></p></td><td class="c63"><p class="c4 c2"><a name="h.ig2gsn60ly02"></a><span>50</span></p></td><td class="c90"><p class="c4 c2"><a name="h.ieswbupikysh"></a><span>50</span></p></td></tr></tbody></table><p class="c1"><a name="h.wju7912zcsbi"></a></p><p class="c2"><a name="h.db9jjmra7drh"></a><span>8/6</span></p><p class="c2"><a name="h.xq6o2p8t3ekh"></a><span>Constitutive promoters</span></p><p class="c1"><a name="h.htlcv52tnfpc"></a></p><p class="c1"><a name="h.l6ban1o0huur"></a></p><a href="#" name="a706356d42cc6cb5710c29f11bea6cdecfddc9c2"></a><a href="#" name="25"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c95"><p class="c4 c2"><a name="h.wibnsic2a910"></a><span>CP Family member (strong)</span></p></td><td class="c98"><p class="c4 c2"><a name="h.6858tthab3pw"></a><span>BBa_J23119</span></p></td><td class="c89"><p class="c4 c2"><a name="h.x1z97dxzq6g7"></a><span>pSBIC3</span></p></td><td class="c17"><p class="c4 c2"><a name="h.gvhoulh69qzn"></a><span>2013 Kit plate 3</span></p></td><td class="c105"><p class="c4 c2"><a name="h.apwq70ggbn27"></a><span>18O</span></p></td><td class="c106"><p class="c4 c2"><a name="h.ints0anm9vhk"></a><span>i5-24-1</span></p></td></tr><tr class="c8"><td class="c95"><p class="c4 c2"><a name="h.t0ps5t1x8q4l"></a><span>CP Family member (medium)</span></p></td><td class="c98"><p class="c4 c2"><a name="h.v56droy9k0d8"></a><span>BBa_K823014 (J23118)</span></p></td><td class="c89"><p class="c4 c2"><a name="h.82leoibdgaqw"></a><span>pSBIC3</span></p></td><td class="c17"><p class="c4 c2"><a name="h.ezuwrzapwbbw"></a><span>2013 Kit plate 1</span></p></td><td class="c105"><p class="c4 c2"><a name="h.3lqdqk898cbd"></a><span>22M</span></p></td><td class="c106"><p class="c4 c2"><a name="h.pek0wjix7k64"></a><span>i5-24-2</span></p></td></tr><tr class="c8"><td class="c95"><p class="c4 c2"><a name="h.s5n956m48pp8"></a><span>CP Family member (weak)</span></p></td><td class="c98"><p class="c4 c2"><a name="h.3kc3i06t27zb"></a><span>BBa_K823012 (J23115)</span></p></td><td class="c89"><p class="c4 c2"><a name="h.jybz3h61sge6"></a><span>pSBIC3</span></p></td><td class="c17"><p class="c4 c2"><a name="h.h8fi7ukqrq2b"></a><span>2013 Kit plate 1</span></p></td><td class="c105"><p class="c4 c2"><a name="h.i92a4rin1bl2"></a><span>22I</span></p></td><td class="c106"><p class="c4 c2"><a name="h.o5q9bg9q4syf"></a><span>i5-24-3</span></p></td></tr></tbody></table><p class="c1"><a name="h.1ncejgt5yklj"></a></p><p class="c2"><a name="h.rycve1ih3gur"></a><span>Transformation </span></p><p class="c2"><a name="h.3jrw5j6p2sgm"></a><span>+ control i5-10-4 → 3 colonies </span></p><p class="c2"><a name="h.adynbsnxormw"></a><span>        i5-24-1 → 0 colonies</span></p><p class="c2"><a name="h.5rytshnqlc72"></a><span>        i5-24-2 → 25 colonies </span></p><p class="c2"><a name="h.k7raf6akjbw7"></a><span>        i5-24-3 → 15+ colonies</span></p><p class="c1"><a name="h.ixvvvk6gweyj"></a></p><p class="c2"><a name="h.5tcllztdk398"></a><span>Gel Extraction </span></p><p class="c1"><a name="h.vfrtwfc8f8cd"></a></p><a href="#" name="3b9b104010f4b2e1060d38a2f95b5f5fd7fba671"></a><a href="#" name="26"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c50"><p class="c1 c4"><a name="h.m0esxbrsc1wj"></a></p></td><td class="c50"><p class="c4 c2"><a name="h.i9bylpzyxh2"></a><span>Concentration (ng/ul)</span></p></td></tr><tr class="c8"><td class="c50"><p class="c4 c2"><a name="h.ints0anm9vhk"></a><span>i5-24-1 Com D</span></p></td><td class="c50"><p class="c4 c2"><a name="h.szs7o03b7gdz"></a><span>17.4</span></p></td></tr><tr class="c8"><td class="c50"><p class="c4 c2"><a name="h.pek0wjix7k64"></a><span>i5-24-2 Com E</span></p></td><td class="c50"><p class="c4 c2"><a name="h.ecp3xchccta6"></a><span>7.6</span></p></td></tr><tr class="c8"><td class="c50"><p class="c4 c2"><a name="h.o5q9bg9q4syf"></a><span>i5-24-3 ComA</span></p></td><td class="c50"><p class="c4 c2"><a name="h.37benhd42xmk"></a><span>7.9</span></p></td></tr><tr class="c8"><td class="c50"><p class="c4 c2"><a name="h.ints0anm9vhk"></a><span>i5-24-4 BackA</span></p></td><td class="c50"><p class="c4 c2"><a name="h.ia9g6gxge9v6"></a><span>13.3</span></p></td></tr><tr class="c8"><td class="c50"><p class="c4 c2"><a name="h.pek0wjix7k64"></a><span>i5-24-5 BackD</span></p></td><td class="c50"><p class="c4 c2"><a name="h.icjpxuqk2dxn"></a><span>11.6</span></p></td></tr><tr class="c8"><td class="c50"><p class="c4 c2"><a name="h.o5q9bg9q4syf"></a><span>i5-24-6 BackE</span></p></td><td class="c50"><p class="c4 c2"><a name="h.x6jb2cbnabnt"></a><span>9.6</span></p></td></tr></tbody></table><p class="c1"><a name="h.a0wn8d1nez2l"></a></p><p class="c2"><a name="h.vh0iwdhh5ns7"></a><span>8/7 </span></p><p class="c2"><a name="h.o7ul5ljt2ywf"></a><span>Inoculate from transformation 8/6 </span></p><p class="c2"><a name="h.2mgh77d71y1j"></a><span>i5-24-2 → 25+ colonies → picked 1 colony → i5-25-7</span></p><p class="c2"><a name="h.62ewmx28f7mt"></a><span>i5-24-3 → 15+ colonies → picked 1 colony → i5-25-8</span></p><p class="c1"><a name="h.yyhxqe7dj6zu"></a></p><p class="c2"><a name="h.iqqfoukq2oz"></a><span>Com D vector backbone dephosphorilation</span></p><p class="c2"><a name="h.h8c9smgqz13j"></a><span>i5-25-5 → i5-25-9 12.5 ng/ul</span></p><p class="c1"><a name="h.hmejr6cbbi1s"></a></p><p class="c2"><a name="h.8qibq2rdsosv"></a><span>Ligation </span></p><p class="c1"><a name="h.cr42wd9mm2t5"></a></p><a href="#" name="92d3062c8ee6a88c6fc20b0ac66d4fb45db13dbe"></a><a href="#" name="27"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c13"><p class="c1 c4"><a name="h.h7dmtux69b0h"></a></p></td><td class="c13"><p class="c4 c2"><a name="h.75fcnjetnhp0"></a><span>Com A 3:1 </span></p></td><td class="c13"><p class="c4 c2"><a name="h.ukmleeioa352"></a><span>Com A 0:1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.g3ghsm5hssqk"></a><span>Com D 3:1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.dnh0x7ywufq7"></a><span>Com D 0:1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.tg789oqh35vb"></a><span>Com E 3:1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.932dpd75v4u"></a><span>Com E 0:1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.u5a3yt7rngho"></a><span>Insert </span></p></td><td class="c13"><p class="c4 c2"><a name="h.bj9gpbdmzw02"></a><span>10</span></p></td><td class="c13"><p class="c4 c2"><a name="h.u3k3id4m92o3"></a><span>0</span></p></td><td class="c13"><p class="c4 c2"><a name="h.7upxcedqi2ni"></a><span>6</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ag8ouhvjt9pj"></a><span>0</span></p></td><td class="c13"><p class="c4 c2"><a name="h.xu0iinttgao0"></a><span>8</span></p></td><td class="c13"><p class="c4 c2"><a name="h.wavjmvuq0zyn"></a><span>0</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.a484e1vh4vez"></a><span>Vector</span></p></td><td class="c13"><p class="c4 c2"><a name="h.x5hvz11tyyru"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.23wofbg7dk9o"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.so5pbs3hicb"></a><span>5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.3mxqleb9s8sn"></a><span>5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.6zldclloa19r"></a><span>5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.kgkhhly07n67"></a><span>5</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.fxjmuca5mbqo"></a><span>Buffer</span></p></td><td class="c13"><p class="c4 c2"><a name="h.684nr6u0aavk"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.f29ne6k35ks0"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.gwt9yiywu8g9"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.9p1fp38hzmrf"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.uw0txhnqieyp"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.blp0k1q8ca3r"></a><span>2</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.eh8s8aai6ccl"></a><span>Ligase</span></p></td><td class="c13"><p class="c4 c2"><a name="h.rdx2fo9pjv50"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.afzclqfltvst"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.jn4ila6bgqp0"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.niyq33tmnvsd"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.mhdr3k2q70c4"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.kdwsnfeco08e"></a><span>1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.jhs7yvjkgipu"></a><span>Water</span></p></td><td class="c13"><p class="c4 c2"><a name="h.98uffvxft24t"></a><span>5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.jx2mq28ndrhq"></a><span>15</span></p></td><td class="c13"><p class="c4 c2"><a name="h.avvmnx6rvcq7"></a><span>6</span></p></td><td class="c13"><p class="c4 c2"><a name="h.jomrcq1rkgq3"></a><span>12</span></p></td><td class="c13"><p class="c4 c2"><a name="h.2t6bgg8pq62k"></a><span>4</span></p></td><td class="c13"><p class="c4 c2"><a name="h.bk5em5zaqqk1"></a><span>12</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.r4gakjxirnm"></a><span>Total</span></p></td><td class="c13"><p class="c4 c2"><a name="h.b7px5p629wb2"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.dr5wsphwugfr"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.1jukh4az3av"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.lv58iogydtel"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.rs9niqua7llp"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.kjbbjk1msd6u"></a><span>20</span></p></td></tr></tbody></table><p class="c1"><a name="h.w7hero9cuyev"></a></p><p class="c2"><a name="h.2j6yxbeu2tab"></a><span>                insert → i5-23-3                        i5-25-1                                i5-25-2</span></p><p class="c2"><a name="h.3o8psi3w6gw"></a><span>                Vector → i5-25-4                        i5-25-9                                i5-25-6</span></p><p class="c2"><a name="h.5yfxj9jlbxfe"></a><span>                1                        2                3                4                5                6</span></p><p class="c1"><a name="h.p6m8jq20861m"></a></p><p class="c2"><a name="h.42bedh5ney9q"></a><span>Transformation </span></p><p class="c2"><a name="h.sn6yo85y7wf5"></a><span>i5-25-1 Com A 3:1 → 0 colonies </span></p><p class="c2"><a name="h.cl44wfisntbw"></a><span>i5-25-2 Com A 0:1 → 0 colonies</span></p><p class="c2"><a name="h.xgujaa4m9ze6"></a><span>i5-25-3 Com D 3:1 → 0 colonies</span></p><p class="c2"><a name="h.lu7vdyxwppfl"></a><span>i5-25-4 Com D 0:1 → 0 colonies </span></p><p class="c2"><a name="h.3kh89f44w1zf"></a><span>i5-25-9 Com E 3:1 →20 colonies → picked 3 colonies → i5-25-10</span></p><p class="c2"><a name="h.iehtfwff6p7f"></a><span>                                                → i5-25-11</span></p><p class="c2"><a name="h.5sydh01237my"></a><span>                                                → - control i5-25-12</span></p><p class="c2"><a name="h.vdvi3f1vqpef"></a><span>i5-25-6 Com E 0:1 → 0 colonies </span></p><p class="c2"><a name="h.7q6omna9ogct"></a><span>i5-11-5 + colonies → plenty colonies </span></p><p class="c1"><a name="h.zcyit1h53l3v"></a></p><p class="c2"><a name="h.86c253es1cm9"></a><span>8/8 </span></p><p class="c2"><a name="h.maa5grwkkatz"></a><span>Made glycerol stock from i5-15-7 and i5-25-8</span></p><p class="c2"><a name="h.ck7tfh7i7pmt"></a><span>Miniprep</span></p><a href="#" name="ec89fb10a3a2f50f8dc02e3b45423d2b3675faa4"></a><a href="#" name="28"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c93"><p class="c1 c4"><a name="h.3jpbcjkctjf6"></a></p></td><td class="c7"><p class="c4 c2"><a name="h.qs5drvo28rm7"></a><span>Concentration (ng/ul)</span></p></td></tr><tr class="c8"><td class="c93"><p class="c4 c2"><a name="h.t0j5ex3dratj"></a><span>i5-25-7 (medium CP)</span></p></td><td class="c7"><p class="c4 c2"><a name="h.91gep83p0uur"></a><span>109.3</span></p></td></tr><tr class="c8"><td class="c93"><p class="c4 c2"><a name="h.cutho5fp38n3"></a><span>i5-25-8 (small CP)</span></p></td><td class="c7"><p class="c4 c2"><a name="h.kxapg9r7ojwl"></a><span>116.7</span></p></td></tr></tbody></table><p class="c1"><a name="h.xtxmx6x6whny"></a></p><p class="c2"><a name="h.bvnzeytbhsfd"></a><span>Transformation</span></p><p class="c2"><a name="h.wg6vhgd3o65d"></a><span>i5-26-1        ComA 3:1</span></p><p class="c2"><a name="h.h76705w0uaeb"></a><span>i5-26-2        ComD 3:1</span></p><p class="c2"><a name="h.r92f30awsl43"></a><span>i5-26-3 pLac-GFP (+ control) </span></p><p class="c2"><a name="h.3dlfciq4c227"></a><span>i5-26-4 ComE 3:1</span></p><p class="c1"><a name="h.aj4foswu0mmv"></a></p><p class="c2"><a name="h.s3iv9m4193k7"></a><span>Digestion </span></p><p class="c1"><a name="h.sa3hn2besxn6"></a></p><a href="#" name="0f6777305afa90a7628766949228ff86f8e7762f"></a><a href="#" name="29"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c3"><p class="c1 c4"><a name="h.1ybxzsmmqky1"></a></p></td><td class="c3"><p class="c4 c2"><a name="h.cztt9ea1hy78"></a><span>i5-26-4</span></p></td><td class="c3"><p class="c4 c2"><a name="h.jxlyl7ugm2hk"></a><span>i5-26-5</span></p></td><td class="c3"><p class="c4 c2"><a name="h.12pcqlm112un"></a><span>i5-26-6</span></p></td><td class="c3"><p class="c4 c2"><a name="h.wk9jf1mpv9w8"></a><span>i5-26-7</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.evm95gi6jtb1"></a><span>DNA</span></p></td><td class="c3"><p class="c4 c2"><a name="h.xzp8jpql5w90"></a><span>30</span></p></td><td class="c3"><p class="c4 c2"><a name="h.43vqez2vhz75"></a><span>11</span></p></td><td class="c3"><p class="c4 c2"><a name="h.vsidxwd6b7mg"></a><span>20</span></p></td><td class="c3"><p class="c4 c2"><a name="h.yxlmuzunsphy"></a><span>20</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.11w80jnnzisx"></a><span>Buffer</span></p></td><td class="c3"><p class="c4 c2"><a name="h.op2rau3vwoi1"></a><span>5</span></p></td><td class="c3"><p class="c4 c2"><a name="h.u5r2g4aik22j"></a><span>5</span></p></td><td class="c3"><p class="c4 c2"><a name="h.93b535wzow5k"></a><span>5</span></p></td><td class="c3"><p class="c4 c2"><a name="h.2ohgeht0b18a"></a><span>5</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.48w8qfo4euj6"></a><span>PstI</span></p></td><td class="c3"><p class="c4 c2"><a name="h.5pgl0skigbuo"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.r168jkjs4pk"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.4djrpkwjmoyu"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.cdxwrql0am71"></a><span>1</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.h6zo64dmnvbu"></a><span>SpcI</span></p></td><td class="c3"><p class="c4 c2"><a name="h.pedgoamanh83"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.bano6kt8m2br"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.df63q63ruwu3"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.4rgl5cj93v1p"></a><span>1</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.veh91msc06en"></a><span>Water</span></p></td><td class="c3"><p class="c4 c2"><a name="h.damc93mc41p6"></a><span>13</span></p></td><td class="c3"><p class="c4 c2"><a name="h.4ss3yymvo82g"></a><span>32</span></p></td><td class="c3"><p class="c4 c2"><a name="h.7uzwotz35pb0"></a><span>23</span></p></td><td class="c3"><p class="c4 c2"><a name="h.2njg98ojeu4w"></a><span>23</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.iwuuy5rg7fcj"></a><span>Total</span></p></td><td class="c3"><p class="c4 c2"><a name="h.82wh9ptmr0k7"></a><span>50</span></p></td><td class="c3"><p class="c4 c2"><a name="h.lzlefu8t5gkh"></a><span>50</span></p></td><td class="c3"><p class="c4 c2"><a name="h.qy4uj2gg7gii"></a><span>50</span></p></td><td class="c3"><p class="c4 c2"><a name="h.6fj46w7i9aw9"></a><span>50</span></p></td></tr></tbody></table><p class="c1"><a name="h.q7os7wcvo5yl"></a></p><p class="c1"><a name="h.8oucsdmb4l50"></a></p><a href="#" name="ef197295390ef46b61c6f71de9807a81f1f2109a"></a><a href="#" name="30"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c42"><p class="c1 c4"><a name="h.7ohb181cvlc5"></a></p></td><td class="c42"><p class="c4 c2"><a name="h.1ndnlsrexn6p"></a><span>i5-26-8</span></p></td><td class="c42"><p class="c4 c2"><a name="h.yvwbj81t7251"></a><span>i5-26-9</span></p></td><td class="c42"><p class="c4 c2"><a name="h.4z2y4vcanl7x"></a><span>i5-26-10</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.bb6yew8hwcb5"></a><span>DNA</span></p></td><td class="c42"><p class="c4 c2"><a name="h.tcf5ai7nvoqi"></a><span>20</span></p></td><td class="c42"><p class="c4 c2"><a name="h.30ln3nwv5bi0"></a><span>20</span></p></td><td class="c42"><p class="c4 c2"><a name="h.dbwm952yixl0"></a><span>20</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.u3c5408277v5"></a><span>Buffer</span></p></td><td class="c42"><p class="c4 c2"><a name="h.4k8p8j95ks0u"></a><span>5</span></p></td><td class="c42"><p class="c4 c2"><a name="h.asyhjt5797kh"></a><span>5</span></p></td><td class="c42"><p class="c4 c2"><a name="h.u0pcfq9fffqb"></a><span>5</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.tqq52md11g1r"></a><span>PstI</span></p></td><td class="c42"><p class="c4 c2"><a name="h.gqx5h1gwtiph"></a><span>1</span></p></td><td class="c42"><p class="c4 c2"><a name="h.8gr973v6w2ij"></a><span>1</span></p></td><td class="c42"><p class="c4 c2"><a name="h.j8dbj5ybpdft"></a><span>1</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.o2auivle7b2"></a><span>XbaI</span></p></td><td class="c42"><p class="c4 c2"><a name="h.s65sqjtdl1fv"></a><span>1</span></p></td><td class="c42"><p class="c4 c2"><a name="h.de99c0rajslu"></a><span>1</span></p></td><td class="c42"><p class="c4 c2"><a name="h.gso7obnhep95"></a><span>1</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.aop280y95qcn"></a><span>Water</span></p></td><td class="c42"><p class="c4 c2"><a name="h.bsebvjwtdzro"></a><span>23</span></p></td><td class="c42"><p class="c4 c2"><a name="h.o5xzj7daaev0"></a><span>23</span></p></td><td class="c42"><p class="c4 c2"><a name="h.2raz43srotx"></a><span>23</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.xy9f3ac7jb3n"></a><span>Total</span></p></td><td class="c42"><p class="c4 c2"><a name="h.z39vfuuiv370"></a><span>50</span></p></td><td class="c42"><p class="c4 c2"><a name="h.ce8zmsqe95xj"></a><span>50</span></p></td><td class="c42"><p class="c4 c2"><a name="h.6p64omi9z28m"></a><span>50</span></p></td></tr></tbody></table><p class="c1"><a name="h.kamtocqtkydk"></a></p><p class="c2"><a name="h.cpb6f9oy218h"></a><span>Transformation </span></p><p class="c2"><a name="h.82l5yurfhh46"></a><span>3:1 ComA</span></p><p class="c2"><a name="h.zc9adneqyswj"></a><span>3:1 ComD</span></p><p class="c2"><a name="h.u4z7e8tb0rrs"></a><span>3:1 ComE</span></p><p class="c2"><a name="h.ib77a1l87t48"></a><span>+ control (pLac-GFP)</span></p><p class="c1"><a name="h.eh7ds6f1gnpm"></a></p><p class="c2"><a name="h.f00tibgergp"></a><span>8/9</span></p><p class="c2"><a name="h.kodcs5othrdp"></a><span>Ligation </span></p><p class="c2"><a name="h.z0kwt5nxopux"></a><span>insert: i5-26-10 Vector i5-26-5</span></p><p class="c1"><a name="h.9h5o97z62ydu"></a></p><a href="#" name="19791e3fc694b2233e965d69ebe788cf41afb465"></a><a href="#" name="31"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c16"><p class="c1 c4"><a name="h.7vluc54wk01l"></a></p></td><td class="c57"><p class="c4 c2"><a name="h.k6mui7b6rz68"></a><span>3:1</span></p></td><td class="c43"><p class="c4 c2"><a name="h.dgvrvsc7bu0g"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c16"><p class="c4 c2"><a name="h.oub71i2pw3n2"></a><span>Insert</span></p></td><td class="c57"><p class="c4 c2"><a name="h.amz5pvvj9q3t"></a><span>6.3</span></p></td><td class="c43"><p class="c4 c2"><a name="h.g3hp3ac60ptf"></a><span>0</span></p></td></tr><tr class="c8"><td class="c16"><p class="c4 c2"><a name="h.ba6duxxfy39o"></a><span>Vector</span></p></td><td class="c57"><p class="c4 c2"><a name="h.60oo94a93h0b"></a><span>2</span></p></td><td class="c43"><p class="c4 c2"><a name="h.n9l5xk276hqv"></a><span>2</span></p></td></tr><tr class="c8"><td class="c16"><p class="c4 c2"><a name="h.yly4vsjehj4"></a><span>Buffer</span></p></td><td class="c57"><p class="c4 c2"><a name="h.m9ba0nwqm5pd"></a><span>2</span></p></td><td class="c43"><p class="c4 c2"><a name="h.z71k2r76mt7f"></a><span>2</span></p></td></tr><tr class="c8"><td class="c16"><p class="c4 c2"><a name="h.abw7saw8owjz"></a><span>Ligase</span></p></td><td class="c57"><p class="c4 c2"><a name="h.q3wgcgmtgmn4"></a><span>1</span></p></td><td class="c43"><p class="c4 c2"><a name="h.6dxg7vck2gbh"></a><span>1</span></p></td></tr><tr class="c8"><td class="c16"><p class="c4 c2"><a name="h.erqt6icosda0"></a><span>Water</span></p></td><td class="c57"><p class="c4 c2"><a name="h.jl1ceixsbhx7"></a><span>8.7</span></p></td><td class="c43"><p class="c4 c2"><a name="h.gfwp6asmt3ku"></a><span>15</span></p></td></tr><tr class="c8"><td class="c16"><p class="c4 c2"><a name="h.3ufubvxg739z"></a><span>Total</span></p></td><td class="c57"><p class="c4 c2"><a name="h.l6j7c6inlwdf"></a><span>20</span></p></td><td class="c43"><p class="c4 c2"><a name="h.zdtgvwkzd1kj"></a><span>20</span></p></td></tr></tbody></table><p class="c2"><a name="h.68jcggnxe2kk"></a><span>                        1        2</span></p><p class="c1"><a name="h.xbyphphtdb9x"></a></p><p class="c2"><a name="h.13k2y8znsdx"></a><span>Insert i5-26-10 Vector i5-25-4</span></p><p class="c1"><a name="h.1pw16fkk8vpm"></a></p><a href="#" name="d17a5321e97ae3ad32f7b3ff7218a6be7531f799"></a><a href="#" name="32"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c33"><p class="c1 c4"><a name="h.peun7r6p3on6"></a></p></td><td class="c54"><p class="c4 c2"><a name="h.asit7cs2rk80"></a><span>3:1</span></p></td><td class="c11"><p class="c4 c2"><a name="h.xgvt5agishz"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c33"><p class="c4 c2"><a name="h.oub71i2pw3n2"></a><span>Insert</span></p></td><td class="c54"><p class="c4 c2"><a name="h.xt55nt6qk32q"></a><span>6.6</span></p></td><td class="c11"><p class="c4 c2"><a name="h.3vknl45azwvf"></a><span>0</span></p></td></tr><tr class="c8"><td class="c33"><p class="c4 c2"><a name="h.ba6duxxfy39o"></a><span>Vector</span></p></td><td class="c54"><p class="c4 c2"><a name="h.d392mwaeapid"></a><span>2.5</span></p></td><td class="c11"><p class="c4 c2"><a name="h.vt7xt8wbfrnz"></a><span>2.5</span></p></td></tr><tr class="c8"><td class="c33"><p class="c4 c2"><a name="h.yly4vsjehj4"></a><span>Buffer</span></p></td><td class="c54"><p class="c4 c2"><a name="h.8gz1siehn7ls"></a><span>2</span></p></td><td class="c11"><p class="c4 c2"><a name="h.m5w9b5bldgpf"></a><span>2</span></p></td></tr><tr class="c8"><td class="c33"><p class="c4 c2"><a name="h.abw7saw8owjz"></a><span>Ligase</span></p></td><td class="c54"><p class="c4 c2"><a name="h.7dv0ve66io28"></a><span>1</span></p></td><td class="c11"><p class="c4 c2"><a name="h.e8rwfes5l0e6"></a><span>1</span></p></td></tr><tr class="c8"><td class="c33"><p class="c4 c2"><a name="h.erqt6icosda0"></a><span>Water</span></p></td><td class="c54"><p class="c4 c2"><a name="h.ygv2qmektbul"></a><span>7.3</span></p></td><td class="c11"><p class="c4 c2"><a name="h.nwq9epaajli6"></a><span>14.5</span></p></td></tr><tr class="c8"><td class="c33"><p class="c4 c2"><a name="h.3ufubvxg739z"></a><span>Total</span></p></td><td class="c54"><p class="c4 c2"><a name="h.k4b8pbveipsm"></a><span>20</span></p></td><td class="c11"><p class="c4 c2"><a name="h.9a8kvehi89fe"></a><span>20</span></p></td></tr></tbody></table><p class="c2"><a name="h.ssbodvtory9t"></a><span>                3                4</span></p><p class="c1"><a name="h.ml1j8fbaxfv8"></a></p><p class="c2"><a name="h.l4rs6e81pgwk"></a><span>Insert i5-26-8  Vector i5-26-6</span></p><p class="c1"><a name="h.4xblm2trv2u8"></a></p><a href="#" name="aaf417d0d211b075e9abdd58751a67903bdd32fa"></a><a href="#" name="33"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c58"><p class="c1 c4"><a name="h.o14kiavtq1a6"></a></p></td><td class="c35"><p class="c4 c2"><a name="h.2p4wl2lj83k8"></a><span>3:1</span></p></td><td class="c53"><p class="c4 c2"><a name="h.1s8hl78y6siy"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c58"><p class="c4 c2"><a name="h.oub71i2pw3n2"></a><span>Insert</span></p></td><td class="c35"><p class="c4 c2"><a name="h.rsxvxg7ie1av"></a><span>4.36</span></p></td><td class="c53"><p class="c4 c2"><a name="h.q6l6h93wnemx"></a><span>0</span></p></td></tr><tr class="c8"><td class="c58"><p class="c4 c2"><a name="h.ba6duxxfy39o"></a><span>Vector</span></p></td><td class="c35"><p class="c4 c2"><a name="h.xhwradv7ofd0"></a><span>2</span></p></td><td class="c53"><p class="c4 c2"><a name="h.hqbqsoydztdo"></a><span>2</span></p></td></tr><tr class="c8"><td class="c58"><p class="c4 c2"><a name="h.yly4vsjehj4"></a><span>Buffer</span></p></td><td class="c35"><p class="c4 c2"><a name="h.5uydunqvx82s"></a><span>2</span></p></td><td class="c53"><p class="c4 c2"><a name="h.lg03ux2ir8a3"></a><span>2</span></p></td></tr><tr class="c8"><td class="c58"><p class="c4 c2"><a name="h.abw7saw8owjz"></a><span>Ligase</span></p></td><td class="c35"><p class="c4 c2"><a name="h.963w6glbch7w"></a><span>1</span></p></td><td class="c53"><p class="c4 c2"><a name="h.avgzsiyct428"></a><span>1</span></p></td></tr><tr class="c8"><td class="c58"><p class="c4 c2"><a name="h.erqt6icosda0"></a><span>Water</span></p></td><td class="c35"><p class="c4 c2"><a name="h.mn8l1ghufu4q"></a><span>10.64</span></p></td><td class="c53"><p class="c4 c2"><a name="h.fip39ewf67qm"></a><span>15</span></p></td></tr><tr class="c8"><td class="c58"><p class="c4 c2"><a name="h.3ufubvxg739z"></a><span>Total</span></p></td><td class="c35"><p class="c4 c2"><a name="h.oz0oalujao1s"></a><span>20</span></p></td><td class="c53"><p class="c4 c2"><a name="h.qt6t1rqi4nbf"></a><span>20</span></p></td></tr></tbody></table><p class="c2"><a name="h.cewwqq7q8bj1"></a><span>                5                6</span></p><p class="c2"><a name="h.4dvgy01tjgh8"></a><span>insert i5-26-9 Vector i5-26-6</span></p><p class="c1"><a name="h.dlzdlm52ue9"></a></p><a href="#" name="d04ab804a74f9748d8914ea8ae7ab6df1f55f82f"></a><a href="#" name="34"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c84"><p class="c1 c4"><a name="h.uk8xljkz43q0"></a></p></td><td class="c14"><p class="c4 c2"><a name="h.rm2a91ypwctm"></a><span>3:1</span></p></td><td class="c44"><p class="c4 c2"><a name="h.rlch06w846tp"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c84"><p class="c4 c2"><a name="h.u924bruxam85"></a><span>insert</span></p></td><td class="c14"><p class="c4 c2"><a name="h.2bhmpi28qiop"></a><span>2.3</span></p></td><td class="c44"><p class="c4 c2"><a name="h.v61xzojcboi6"></a><span>0</span></p></td></tr><tr class="c8"><td class="c84"><p class="c4 c2"><a name="h.fdocnt4y7ji3"></a><span>vector</span></p></td><td class="c14"><p class="c4 c2"><a name="h.sjk306r40m4u"></a><span>2</span></p></td><td class="c44"><p class="c4 c2"><a name="h.se475ae81qyn"></a><span>2</span></p></td></tr><tr class="c8"><td class="c84"><p class="c4 c2"><a name="h.t4a7k41jiwj"></a><span>buffer</span></p></td><td class="c14"><p class="c4 c2"><a name="h.o3qtri6whr8e"></a><span>2</span></p></td><td class="c44"><p class="c4 c2"><a name="h.e5p264vlll3s"></a><span>2</span></p></td></tr><tr class="c8"><td class="c84"><p class="c4 c2"><a name="h.7cppxveawh2b"></a><span>ligase</span></p></td><td class="c14"><p class="c4 c2"><a name="h.a29fc4w34toq"></a><span>1</span></p></td><td class="c44"><p class="c4 c2"><a name="h.e99nn4f6ts9w"></a><span>1</span></p></td></tr><tr class="c8"><td class="c84"><p class="c4 c2"><a name="h.ob97c8cj4okf"></a><span>water</span></p></td><td class="c14"><p class="c4 c2"><a name="h.ze8koa8orksh"></a><span>12.7</span></p></td><td class="c44"><p class="c4 c2"><a name="h.f77rwsefozr5"></a><span>15</span></p></td></tr><tr class="c8"><td class="c84"><p class="c4 c2"><a name="h.1qxb72m36rfj"></a><span>total</span></p></td><td class="c14"><p class="c4 c2"><a name="h.8b6ffl6k30mh"></a><span>20</span></p></td><td class="c44"><p class="c4 c2"><a name="h.chvq5gaw562h"></a><span>20</span></p></td></tr></tbody></table><p class="c2"><a name="h.5h8su0c8cvoe"></a><span>                7                8</span></p><p class="c1"><a name="h.3g8l51vx5mmt"></a></p><p class="c2"><a name="h.31elyj5r49k7"></a><span>Transformation</span></p><p class="c2"><a name="h.v1z8vn6xu3il"></a><span>Control (w/ GFP) [i5-10-1] → i5-28-1</span></p><p class="c2"><a name="h.qakg30ck28x3"></a><span>Control (w/out GFP) [i5-22-2] → i5-28-2</span></p><p class="c1"><a name="h.9d158dy3dj5z"></a></p><p class="c2"><a name="h.tz9j1wbg7mr3"></a><span>1 → i5-28-3</span></p><p class="c2"><a name="h.8il6mtefdzrt"></a><span>2 → i5-28-4</span></p><p class="c2"><a name="h.747dg3btie74"></a><span>3 → i5-28-5</span></p><p class="c2"><a name="h.9540h4uswcxa"></a><span>4 → i5-28-6        no growth </span></p><p class="c2"><a name="h.lrkw3udcpxuo"></a><span>5 → i5-28-7</span></p><p class="c2"><a name="h.58jyy6t51cy7"></a><span>6 → i5-28-8</span></p><p class="c2"><a name="h.vwu0q7nzmq1k"></a><span>7→ i5-28-9</span></p><p class="c2"><a name="h.2zsv5y78rfix"></a><span>8 → i5-28-10 </span></p><p class="c1"><a name="h.hbt714lmlqv9"></a></p><p class="c2"><a name="h.73fhqn2jb5kj"></a><span>Ligation</span></p><p class="c2"><a name="h.g3cakqv1n2j"></a><span>redo from 8/7</span></p><p class="c1"><a name="h.213inz40gxm2"></a></p><p class="c2"><a name="h.5qyjxe42jdd2"></a><span>Transformation </span></p><p class="c2"><a name="h.te5asa59w3uc"></a><span>ComA 3:1</span></p><p class="c2"><a name="h.49y1cm5hrwwa"></a><span>Com A 0:1</span></p><p class="c2"><a name="h.4m8d55jvn825"></a><span>Com D 3:1</span></p><p class="c2"><a name="h.4afpflrq2tlb"></a><span>Com D 0:1</span></p><p class="c2"><a name="h.6s97dwn6g7av"></a><span>ComE 3:1</span></p><p class="c2"><a name="h.euuof7rbjt5n"></a><span>Com E 0:1</span></p><p class="c2"><a name="h.1e00fxu6rigc"></a><span>pLac-GFP as + control</span></p><p class="c1"><a name="h.r9jiwbxkqjp"></a></p><p class="c2"><a name="h.8asdz9ocehq7"></a><span>8/10 </span></p><p class="c2"><a name="h.9tu8znned25x"></a><span>ligation </span></p><p class="c2"><a name="h.7ggfrz5jwixt"></a><span>insert: i5-26-10 Vector i5-26-5</span></p><p class="c1"><a name="h.fs330v6uzncj"></a></p><a href="#" name="8f02dfea07dc25a4d7910b4bcdabaf0828fd7502"></a><a href="#" name="35"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c60"><p class="c1 c4"><a name="h.lr1ucm3je5ch"></a></p></td><td class="c34"><p class="c4 c2"><a name="h.1tdfkaxf72hu"></a><span>3:1</span></p></td><td class="c11"><p class="c4 c2"><a name="h.irgy751q45bb"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.29frdg7sf7qs"></a><span>insert</span></p></td><td class="c34"><p class="c4 c2"><a name="h.3jo10h4lfbwa"></a><span>6.3</span></p></td><td class="c11"><p class="c4 c2"><a name="h.66r7wdylz39h"></a><span>0</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.grelxwnto9yw"></a><span>vector</span></p></td><td class="c34"><p class="c4 c2"><a name="h.3uzirpqv6238"></a><span>2</span></p></td><td class="c11"><p class="c4 c2"><a name="h.f2tp69mzqtu8"></a><span>2</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.mr4s0tbd3zsx"></a><span>buffer</span></p></td><td class="c34"><p class="c4 c2"><a name="h.5d0nzx5y3dd7"></a><span>2</span></p></td><td class="c11"><p class="c4 c2"><a name="h.b7g51hyqpul1"></a><span>2</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.voe786qcl1rf"></a><span>ligase</span></p></td><td class="c34"><p class="c4 c2"><a name="h.q5ym06cr9hin"></a><span>1</span></p></td><td class="c11"><p class="c4 c2"><a name="h.o7p5fhdl1qx2"></a><span>1</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.kvnjo4exssvp"></a><span>water</span></p></td><td class="c34"><p class="c4 c2"><a name="h.xaalwyd7nrzb"></a><span>8.7</span></p></td><td class="c11"><p class="c4 c2"><a name="h.8xb8uwpml5mk"></a><span>15</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.rrlxvuele5sw"></a><span>total</span></p></td><td class="c34"><p class="c4 c2"><a name="h.mwokkpi7pthu"></a><span>20</span></p></td><td class="c11"><p class="c4 c2"><a name="h.xke9jmf50d4i"></a><span>20</span></p></td></tr></tbody></table><p class="c2"><a name="h.mrcdf6e8qi1q"></a><span>                1                2</span></p><p class="c2"><a name="h.8pcb8d35m6as"></a><span>insert i5-26-10 vector i5-26-4</span></p><p class="c1"><a name="h.fs330v6uzncj"></a></p><a href="#" name="d9c4c682c5375fd0d45dd4d7f3730aaa47820b8a"></a><a href="#" name="36"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c60"><p class="c1 c4"><a name="h.lr1ucm3je5ch"></a></p></td><td class="c34"><p class="c4 c2"><a name="h.1tdfkaxf72hu"></a><span>3:1</span></p></td><td class="c11"><p class="c4 c2"><a name="h.irgy751q45bb"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.29frdg7sf7qs"></a><span>insert</span></p></td><td class="c34"><p class="c4 c2"><a name="h.3jo10h4lfbwa"></a><span>6.6</span></p></td><td class="c11"><p class="c4 c2"><a name="h.66r7wdylz39h"></a><span>0</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.grelxwnto9yw"></a><span>vector</span></p></td><td class="c34"><p class="c4 c2"><a name="h.3uzirpqv6238"></a><span>2.5</span></p></td><td class="c11"><p class="c4 c2"><a name="h.f2tp69mzqtu8"></a><span>2.5</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.mr4s0tbd3zsx"></a><span>buffer</span></p></td><td class="c34"><p class="c4 c2"><a name="h.5d0nzx5y3dd7"></a><span>2</span></p></td><td class="c11"><p class="c4 c2"><a name="h.b7g51hyqpul1"></a><span>2</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.voe786qcl1rf"></a><span>ligase</span></p></td><td class="c34"><p class="c4 c2"><a name="h.q5ym06cr9hin"></a><span>1</span></p></td><td class="c11"><p class="c4 c2"><a name="h.o7p5fhdl1qx2"></a><span>1</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.kvnjo4exssvp"></a><span>water</span></p></td><td class="c34"><p class="c4 c2"><a name="h.xaalwyd7nrzb"></a><span>7.3</span></p></td><td class="c11"><p class="c4 c2"><a name="h.8xb8uwpml5mk"></a><span>14.5</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.rrlxvuele5sw"></a><span>total</span></p></td><td class="c34"><p class="c4 c2"><a name="h.mwokkpi7pthu"></a><span>20</span></p></td><td class="c11"><p class="c4 c2"><a name="h.xke9jmf50d4i"></a><span>20</span></p></td></tr></tbody></table><p class="c2"><a name="h.2zzkjr266vom"></a><span>                3                4</span></p><p class="c2"><a name="h.c7ej88x5dknt"></a><span>insert: i5-26-8 vector: i5-26-6</span></p><p class="c1"><a name="h.75jan7t5a5wa"></a></p><a href="#" name="fb06182ab4eca39bc376798c31660a3ff39e3dbc"></a><a href="#" name="37"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c49"><p class="c1 c4"><a name="h.rp0xx4ndzdnc"></a></p></td><td class="c54"><p class="c4 c2"><a name="h.mcz4896ozeum"></a><span>3:1</span></p></td><td class="c59"><p class="c4 c2"><a name="h.vxgp54ojxuvj"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c49"><p class="c4 c2"><a name="h.qausojphcizg"></a><span>insert</span></p></td><td class="c54"><p class="c4 c2"><a name="h.9nynadi0m07"></a><span>4.4</span></p></td><td class="c59"><p class="c4 c2"><a name="h.tqh3z8gi5eem"></a><span>0</span></p></td></tr><tr class="c8"><td class="c49"><p class="c4 c2"><a name="h.g8daez7kpm6m"></a><span>vector</span></p></td><td class="c54"><p class="c4 c2"><a name="h.zbn5uiicfri2"></a><span>2</span></p></td><td class="c59"><p class="c4 c2"><a name="h.de9a00gs89ot"></a><span>2</span></p></td></tr><tr class="c8"><td class="c49"><p class="c4 c2"><a name="h.qc6mrhqcnlyr"></a><span>buffer</span></p></td><td class="c54"><p class="c4 c2"><a name="h.pcgj2fa8t2fk"></a><span>2</span></p></td><td class="c59"><p class="c4 c2"><a name="h.lz8uvgragy7d"></a><span>2</span></p></td></tr><tr class="c8"><td class="c49"><p class="c4 c2"><a name="h.nxwjrpkmzq6v"></a><span>ligase</span></p></td><td class="c54"><p class="c4 c2"><a name="h.atwez3jpqgqh"></a><span>1</span></p></td><td class="c59"><p class="c4 c2"><a name="h.knj4v3lna52d"></a><span>1</span></p></td></tr><tr class="c8"><td class="c49"><p class="c4 c2"><a name="h.3mc1x2tvomwu"></a><span>water</span></p></td><td class="c54"><p class="c4 c2"><a name="h.yuz1fyimsmsf"></a><span>10.6</span></p></td><td class="c59"><p class="c4 c2"><a name="h.6bzfezftr4lh"></a><span>15</span></p></td></tr><tr class="c8"><td class="c49"><p class="c4 c2"><a name="h.eqqsvyoz2beq"></a><span>total</span></p></td><td class="c54"><p class="c4 c2"><a name="h.slsnq3q69jdh"></a><span>20</span></p></td><td class="c59"><p class="c4 c2"><a name="h.za6kz0jioq5y"></a><span>20</span></p></td></tr></tbody></table><p class="c2"><a name="h.5jjln8h6k8kb"></a><span>                        5                6</span></p><p class="c2"><a name="h.y7etqamfqm6a"></a><span>insert i5-26-9 Vector i5-26-6</span></p><p class="c1"><a name="h.j08bvg6vg5b6"></a></p><a href="#" name="2b6ea62b0c59c28b37143d040b37dd7b568cb551"></a><a href="#" name="38"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c101"><p class="c1 c4"><a name="h.o9c2o0qsvdh"></a></p></td><td class="c59"><p class="c4 c2"><a name="h.xr45vdntahox"></a><span>3:1</span></p></td></tr><tr class="c8"><td class="c101"><p class="c4 c2"><a name="h.xg71pdalpo99"></a><span>insert</span></p></td><td class="c59"><p class="c4 c2"><a name="h.9i2exop4xi3i"></a><span>5.1</span></p></td></tr><tr class="c8"><td class="c101"><p class="c4 c2"><a name="h.tnj7o45aslz6"></a><span>vector</span></p></td><td class="c59"><p class="c4 c2"><a name="h.4jq6y5rz3ega"></a><span>2</span></p></td></tr><tr class="c8"><td class="c101"><p class="c4 c2"><a name="h.8rl0imxjpnln"></a><span>buffer</span></p></td><td class="c59"><p class="c4 c2"><a name="h.h7ae1fba1ius"></a><span>2</span></p></td></tr><tr class="c8"><td class="c101"><p class="c4 c2"><a name="h.3n097hgtubt9"></a><span>ligase</span></p></td><td class="c59"><p class="c4 c2"><a name="h.1h6286lbzxyx"></a><span>1</span></p></td></tr><tr class="c8"><td class="c101"><p class="c4 c2"><a name="h.6j9jobvb4735"></a><span>water</span></p></td><td class="c59"><p class="c4 c2"><a name="h.jdxhm6vm65zt"></a><span>9.9</span></p></td></tr><tr class="c8"><td class="c101"><p class="c4 c2"><a name="h.qf5w6k7j91ln"></a><span>total</span></p></td><td class="c59"><p class="c4 c2"><a name="h.7srltd35ouat"></a><span>20</span></p></td></tr></tbody></table><p class="c2"><a name="h.2oy2xb6en0p2"></a><span>                7</span></p><p class="c2"><a name="h.sio52xcr0lwf"></a><span>8/10</span></p><p class="c2"><a name="h.424r9tg71t6"></a><span>Transformation </span></p><p class="c2"><a name="h.qv53featg3ve"></a><span>1 → i5-30-1 → 5+ colonies [pFru-RBS-GFP]</span></p><p class="c2"><a name="h.2mrodfqf4cj5"></a><span>2 → i5-30-2 → 0 colonies</span></p><p class="c2"><a name="h.h1ailh1sd55k"></a><span>3 → i5-30-3 → 5 colonies f[pScr-RBS-GFP]</span></p><p class="c2"><a name="h.h9xnfa7sv8gj"></a><span>4 → i5-30-4→ 0 colonies </span></p><p class="c2"><a name="h.f5rdsf7n4y3l"></a><span>5 → i5-30-5 → 60+ colonies [CP-Cra]</span></p><p class="c2"><a name="h.64iburyxf127"></a><span>6 → i5-30-6 → 60+ colonies </span></p><p class="c2"><a name="h.bi19hrunzili"></a><span>7 → i5-30-7 → 60+ colonies  [CP-ScR]</span></p>	+	<p class="c2"><span>8/11</span></p><p class="c2"><a name="h.vvmiq3irzx74"></a><span>picking colonies from transformation 8/10</span></p><p class="c1"><a name="h.xcc9g4d1gada"></a></p><p class="c2"><a name="h.olbwgp15g6t9"></a><span>i5-30-3 → i5-30-8</span></p><p class="c2"><a name="h.21q6gds1ehex"></a><span>        i5-30-9</span></p><p class="c1"><a name="h.bzlm1jlyjh80"></a></p><p class="c2"><a name="h.5wc3l82d0jxe"></a><span>Inoculate from glycerol stock:</span></p><p class="c2"><a name="h.4vfyb030afzi"></a><span>i5-22-5 [Scr]</span></p><p class="c2"><a name="h.t60w4w1qz78e"></a><span>i5-11-5 [pLac-GFP]</span></p><p class="c1"><a name="h.jmmhjdd89zr4"></a></p><p class="c2"><a name="h.sthj625sbh1j"></a><span>1 → i5-11-5        61.1</span></p><p class="c2"><a name="h.9r999ot36cnq"></a><span>2 → i5-11-5        70.5</span></p><p class="c2"><a name="h.32qb54z130re"></a><span>3 → i5-11-5        58.6</span></p><p class="c2"><a name="h.db6xyee79l2m"></a><span>4 → i5-11-5        53.2</span></p><p class="c2"><a name="h.6eijjl1so9ik"></a><span>5 → i5-11-5         89.5</span></p><p class="c1"><a name="h.hgiet1dz05mf"></a></p><p class="c2"><a name="h.5dtixuf80mfh"></a><span>Digestion </span></p><p class="c1"><a name="h.i3wavv6tqk2b"></a></p><a href="#" name="c3a2081ed35a86c2714ec99c9835d13d47592d1a"></a><a href="#" name="39"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c3"><p class="c1 c4"><a name="h.8vt5g222tbak"></a></p></td><td class="c3"><p class="c4 c2"><a name="h.riiwupqmqcoi"></a><span>i5-31-1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.jsoa1kpxc4vv"></a><span>i5-31-2</span></p></td><td class="c3"><p class="c4 c2"><a name="h.5ruk5040g5t4"></a><span>i5-31-3</span></p></td><td class="c3"><p class="c4 c2"><a name="h.u0empn399lsg"></a><span>i5-31-4/5</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.hnfez9nn2o8k"></a><span>Dna</span></p></td><td class="c3"><p class="c4 c2"><a name="h.22wlwhnoii02"></a><span>ComA (61.1) 32.7</span></p></td><td class="c3"><p class="c4 c2"><a name="h.ivc0psafpm3j"></a><span>ComD (70) 28.5 </span></p></td><td class="c3"><p class="c4 c2"><a name="h.duls23yq14bd"></a><span>ComE (58.6) 34.1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.pr2gmd48fifd"></a><span>Dex 30</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.fcegagyihx5l"></a><span>water</span></p></td><td class="c3"><p class="c4 c2"><a name="h.h60bu4sdaasu"></a><span>11</span></p></td><td class="c3"><p class="c4 c2"><a name="h.nfgfyiefc5up"></a><span>15</span></p></td><td class="c3"><p class="c4 c2"><a name="h.be22s9c5w1j6"></a><span>10</span></p></td><td class="c3"><p class="c4 c2"><a name="h.lecx4rtgqph2"></a><span>13</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.sw6apjw78neq"></a><span>Buffer</span></p></td><td class="c3"><p class="c4 c2"><a name="h.mhn3h9cykay"></a><span>5</span></p></td><td class="c3"><p class="c4 c2"><a name="h.3cyhgka656mz"></a><span>5</span></p></td><td class="c3"><p class="c4 c2"><a name="h.7ys3s1iv9zj8"></a><span>5</span></p></td><td class="c3"><p class="c4 c2"><a name="h.4d9gi6dnihxw"></a><span>5</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.vxbk98y03h1i"></a><span>EcoR</span></p></td><td class="c3"><p class="c4 c2"><a name="h.vj3akwf13l2h"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.z6mcgyoplpso"></a><span>0</span></p></td><td class="c3"><p class="c4 c2"><a name="h.k133bsa79gki"></a><span>0</span></p></td><td class="c3"><p class="c4 c2"><a name="h.jqxb8sunh8wq"></a><span>0</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.kkvqfofng39p"></a><span>PstI</span></p></td><td class="c3"><p class="c4 c2"><a name="h.w6m4vgeqx9h5"></a><span>0</span></p></td><td class="c3"><p class="c4 c2"><a name="h.rd9ij47q4kjx"></a><span>0</span></p></td><td class="c3"><p class="c4 c2"><a name="h.dw7g5lxl7xhz"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.bm7ymci4yk4a"></a><span>0</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.5tckkbw5ia12"></a><span>XbaI</span></p></td><td class="c3"><p class="c4 c2"><a name="h.bc4tflx1zoj8"></a><span>0</span></p></td><td class="c3"><p class="c4 c2"><a name="h.scgko0f8q9w7"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.o13kh3yf1av4"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.tbrncavf235g"></a><span>1</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.5uedgdiksfu6"></a><span>SpcI</span></p></td><td class="c3"><p class="c4 c2"><a name="h.n2xj9c6hsus1"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.k6jj4syfvv0l"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.swtckdt4933u"></a><span>0</span></p></td><td class="c3"><p class="c4 c2"><a name="h.92mfu5ky8kx8"></a><span>1</span></p></td></tr></tbody></table><p class="c1"><a name="h.2y54s12xhlbx"></a></p><p class="c2"><a name="h.ugixtn8vvmug"></a><span>i5-31-1                16.5</span></p><p class="c2"><a name="h.7wuuci41molh"></a><span>i5-31-2                10.3</span></p><p class="c2"><a name="h.nfcfce2p4r66"></a><span>i5-31-3                21.0</span></p><p class="c2"><a name="h.1lia2mtp2vk9"></a><span>i5-31-5                12.1</span></p><p class="c1"><a name="h.tidyk8jqpoz9"></a></p><p class="c2"><a name="h.c8mms58srvew"></a><span>8/12</span></p><p class="c2"><a name="h.z60iu2l2d4j"></a><span>Made glycerol stock of:</span></p><p class="c2"><a name="h.si7nyr3khs9e"></a><span>i5-30-1 [pFru-RBS-GFP]</span></p><p class="c2"><a name="h.dfxrx1h9mii1"></a><span>i5-30-8 [pScr-RBS-GFP]</span></p><p class="c2"><a name="h.xja03ev51qni"></a><span>i5-30-9  [pScr-RBS-GFP]</span></p><p class="c2"><a name="h.popdmbz54bhh"></a><span>i5-30-5 [CP-Cra] (wrong, redid 8/16)</span></p><p class="c2"><a name="h.rufx1ux1fjhd"></a><span>i5-30-7 [CP-Scr]</span></p><p class="c1"><a name="h.9awna8x2h0rd"></a></p><p class="c2"><a name="h.mw6tnc4qqkw0"></a><span>Miniprep</span></p><p class="c2"><a name="h.si7nyr3khs9e"></a><span>i5-30-1 → 53.2 [pFru-RBS-GFP]</span></p><p class="c2"><a name="h.xmkvqj7cif62"></a><span>i5-30-8 → 82.0  [pScr-RBS-GFP]</span></p><p class="c2"><a name="h.xja03ev51qni"></a><span>i5-30-9 → 92.3</span></p><p class="c2"><a name="h.popdmbz54bhh"></a><span>i5-30-5 → 78.2</span></p><p class="c2"><a name="h.mzxfdsv7dj3u"></a><span>i5-30-7 → 56.8</span></p><p class="c2"><a name="h.bb2xhfn87ro0"></a><span>Ligation </span></p><p class="c1"><a name="h.kw05m9lhb1km"></a></p><a href="#" name="52cc767bd298850806f2de9dd11c9f4d6597834f"></a><a href="#" name="40"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c85"><p class="c1 c4"><a name="h.cfli8oc53zij"></a></p></td><td class="c65"><p class="c4 c2"><a name="h.tnl4yi53q6q5"></a><span>ComA</span></p></td><td class="c11"><p class="c4 c2"><a name="h.s672t8di70x2"></a><span>ComA</span></p></td><td class="c71"><p class="c4 c2"><a name="h.xamf4p3sdygb"></a><span>ComD</span></p></td><td class="c11"><p class="c4 c2"><a name="h.xhqfijsu5a81"></a><span>ComD</span></p></td><td class="c10"><p class="c4 c2"><a name="h.iodh2qkxwijg"></a><span>ComE</span></p></td><td class="c22"><p class="c4 c2"><a name="h.q0dxynqaozbt"></a><span>ComE</span></p></td><td class="c54"><p class="c4 c2"><a name="h.1jdaufvz7q36"></a><span>Dex</span></p></td></tr><tr class="c8"><td class="c85"><p class="c1 c4"><a name="h.wqiqbk8r8gd1"></a></p></td><td class="c65"><p class="c4 c2"><a name="h.7qpzbext29gi"></a><span>3:1</span></p></td><td class="c11"><p class="c4 c2"><a name="h.paqkalvi7ivq"></a><span>0:1</span></p></td><td class="c71"><p class="c4 c2"><a name="h.py5u8i4ld045"></a><span>3:1</span></p></td><td class="c11"><p class="c4 c2"><a name="h.98umyyb6hufi"></a><span>0:1</span></p></td><td class="c10"><p class="c4 c2"><a name="h.4zn9fywnaldl"></a><span>3:1</span></p></td><td class="c22"><p class="c4 c2"><a name="h.bowuoxuc9v03"></a><span>0:1</span></p></td><td class="c54"><p class="c4 c2"><a name="h.b0bqolnt4a82"></a><span>3:1</span></p></td></tr><tr class="c8"><td class="c85"><p class="c4 c2"><a name="h.wqiqbk8r8gd1"></a><span>Insert</span></p></td><td class="c65"><p class="c4 c2"><a name="h.cxlvspvsbbq6"></a><span>10</span></p></td><td class="c11"><p class="c4 c2"><a name="h.cx070p8ko9dq"></a><span>0</span></p></td><td class="c71"><p class="c4 c2"><a name="h.mxqfbwczspx9"></a><span>6</span></p></td><td class="c11"><p class="c4 c2"><a name="h.x6sl1wi2rmrg"></a><span>0</span></p></td><td class="c10"><p class="c4 c2"><a name="h.j32dv9vyty22"></a><span>8</span></p></td><td class="c22"><p class="c4 c2"><a name="h.5y5ferwcv6x6"></a><span>0</span></p></td><td class="c54"><p class="c4 c2"><a name="h.vq89odwvolxf"></a><span>6.75</span></p></td></tr><tr class="c8"><td class="c85"><p class="c4 c2"><a name="h.ncf56p5a20ln"></a><span>Vector</span></p></td><td class="c65"><p class="c4 c2"><a name="h.x79eciedmwz0"></a><span>3</span></p></td><td class="c11"><p class="c4 c2"><a name="h.ye8nbvfo344y"></a><span>3</span></p></td><td class="c71"><p class="c4 c2"><a name="h.oql65oi32idv"></a><span>5</span></p></td><td class="c11"><p class="c4 c2"><a name="h.l0e0tk65fc7h"></a><span>5</span></p></td><td class="c10"><p class="c4 c2"><a name="h.bqxjrvhy0zyn"></a><span>2.5</span></p></td><td class="c22"><p class="c4 c2"><a name="h.fzqgneabxu1r"></a><span>2.5</span></p></td><td class="c54"><p class="c4 c2"><a name="h.gbqzzss4ic"></a><span>2</span></p></td></tr><tr class="c8"><td class="c85"><p class="c4 c2"><a name="h.w5xhsa8ln4s"></a><span>Buffer</span></p></td><td class="c65"><p class="c4 c2"><a name="h.o6exq9icnoew"></a><span>2</span></p></td><td class="c11"><p class="c4 c2"><a name="h.8lvpkmnzfss9"></a><span>2</span></p></td><td class="c71"><p class="c4 c2"><a name="h.n1eas5or9s5"></a><span>2</span></p></td><td class="c11"><p class="c4 c2"><a name="h.f1brl88flh57"></a><span>2</span></p></td><td class="c10"><p class="c4 c2"><a name="h.h6f75tjy0f0x"></a><span>2</span></p></td><td class="c22"><p class="c4 c2"><a name="h.cbk9vwmh6eq4"></a><span>2</span></p></td><td class="c54"><p class="c4 c2"><a name="h.5sp623sblow0"></a><span>2</span></p></td></tr><tr class="c8"><td class="c85"><p class="c4 c2"><a name="h.side06m7hs1z"></a><span>Ligase</span></p></td><td class="c65"><p class="c4 c2"><a name="h.vf7x1ponl3ce"></a><span>1</span></p></td><td class="c11"><p class="c4 c2"><a name="h.57ieivf2yila"></a><span>1</span></p></td><td class="c71"><p class="c4 c2"><a name="h.nlpzer8ivilv"></a><span>1</span></p></td><td class="c11"><p class="c4 c2"><a name="h.9qwnd76rc8te"></a><span>1</span></p></td><td class="c10"><p class="c4 c2"><a name="h.yzv148wv3jtn"></a><span>1</span></p></td><td class="c22"><p class="c4 c2"><a name="h.xq801lqz9aqz"></a><span>1</span></p></td><td class="c54"><p class="c4 c2"><a name="h.t1kzlcyz6tp0"></a><span>1</span></p></td></tr><tr class="c8"><td class="c85"><p class="c4 c2"><a name="h.eryxzcf41if6"></a><span>Water</span></p></td><td class="c65"><p class="c4 c2"><a name="h.oin1a99r6bel"></a><span>4</span></p></td><td class="c11"><p class="c4 c2"><a name="h.o27rrghxlr2r"></a><span>14</span></p></td><td class="c71"><p class="c4 c2"><a name="h.x8hkiphev4lz"></a><span>6</span></p></td><td class="c11"><p class="c4 c2"><a name="h.iemp08k16607"></a><span>12</span></p></td><td class="c10"><p class="c4 c2"><a name="h.ofephv51plgo"></a><span>6.5</span></p></td><td class="c22"><p class="c4 c2"><a name="h.1bamdauhn2ub"></a><span>14.5</span></p></td><td class="c54"><p class="c4 c2"><a name="h.uk544tp0w364"></a><span>8.25</span></p></td></tr><tr class="c8"><td class="c85"><p class="c4 c2"><a name="h.w8c2gngxwsbd"></a><span>Total</span></p></td><td class="c65"><p class="c4 c2"><a name="h.4fo03sz9g275"></a><span>20</span></p></td><td class="c11"><p class="c4 c2"><a name="h.dp33idhscmtm"></a><span>20</span></p></td><td class="c71"><p class="c4 c2"><a name="h.sk7070l7diu1"></a><span>20</span></p></td><td class="c11"><p class="c4 c2"><a name="h.ecmr2drb5drx"></a><span>20</span></p></td><td class="c10"><p class="c4 c2"><a name="h.gbkwro9b8qff"></a><span>20</span></p></td><td class="c22"><p class="c4 c2"><a name="h.nyhm7ymibitx"></a><span>20</span></p></td><td class="c54"><p class="c4 c2"><a name="h.9t06fea2a72p"></a><span>20</span></p></td></tr><tr class="c8"><td class="c85"><p class="c1 c4"><a name="h.557lenwh4acz"></a></p></td><td class="c65"><p class="c4 c2"><a name="h.oqmynkqtai6z"></a><span>Insert: i5-25-3</span></p><p class="c4 c2"><a name="h.bnwzdca05ak9"></a><span>Vector: i5-31-1</span></p></td><td class="c11"><p class="c4 c2"><a name="h.oqmynkqtai6z"></a><span>I: i5-25-3</span></p><p class="c4 c2"><a name="h.bnwzdca05ak9"></a><span>V: i5-31-1</span></p></td><td class="c71"><p class="c4 c2"><a name="h.ya3rzcfa7g47"></a><span>I: i5-25-1</span></p><p class="c4 c2"><a name="h.kca81p3gmj9k"></a><span>V:i5-31-2</span></p></td><td class="c11"><p class="c4 c2"><a name="h.ya3rzcfa7g47"></a><span>I: i5-25-1</span></p><p class="c4 c2"><a name="h.kca81p3gmj9k"></a><span>V:i5-31-2</span></p></td><td class="c10"><p class="c4 c2"><a name="h.qauaamczt8gp"></a><span>I: i5-25-2</span></p><p class="c4 c2"><a name="h.8li0bnudb2c4"></a><span>V: i5-31-3</span></p></td><td class="c22"><p class="c4 c2"><a name="h.qauaamczt8gp"></a><span>I: i5-25-2</span></p><p class="c4 c2"><a name="h.8li0bnudb2c4"></a><span>V: i5-31-3</span></p></td><td class="c54"><p class="c4 c2"><a name="h.qauaamczt8gp"></a><span>I: i5-31-5</span></p><p class="c4 c2"><a name="h.8li0bnudb2c4"></a><span>V: i5-31-2</span></p></td></tr><tr class="c8"><td class="c85"><p class="c1 c4"><a name="h.edscmbgtd87j"></a></p></td><td class="c65"><p class="c4 c2"><a name="h.224van5pf5l1"></a><span>1</span></p></td><td class="c11"><p class="c4 c2"><a name="h.382dg1rr800e"></a><span>2</span></p></td><td class="c71"><p class="c4 c2"><a name="h.tid93xvoiipv"></a><span>3</span></p></td><td class="c11"><p class="c4 c2"><a name="h.epzohjyq4x2c"></a><span>4</span></p></td><td class="c10"><p class="c4 c2"><a name="h.n2no183w2p1q"></a><span>5</span></p></td><td class="c22"><p class="c4 c2"><a name="h.q15mpoqb3srg"></a><span>6</span></p></td><td class="c54"><p class="c4 c2"><a name="h.oecqb87ui6x9"></a><span>7</span></p></td></tr></tbody></table><p class="c1"><a name="h.p3lwslkhszap"></a></p><p class="c2"><a name="h.o9ndf69qcz4j"></a><span>Transformation</span></p><p class="c2"><a name="h.w2q77lo5hvc1"></a><span>1 →ComA 3:1</span></p><p class="c2"><a name="h.720ozsgw6h93"></a><span>2→ComA 0:1</span></p><p class="c2"><a name="h.83lcczouup6g"></a><span>3→ComD3:1</span></p><p class="c2"><a name="h.onc7rknpledz"></a><span>4→ComD 0:1</span></p><p class="c2"><a name="h.4ficwey6o8ig"></a><span>5→ComE 3:1</span></p><p class="c2"><a name="h.6l83pdf75g42"></a><span>6→ComE 0:1</span></p><p class="c2"><a name="h.mq17t4ufsjk"></a><span>7→Dex → 1 colony</span></p><p class="c2"><a name="h.d6xm84vr4x83"></a><span>8→ + control → 100+ colonies</span></p><p class="c1"><a name="h.afwnx03l7tn1"></a></p><p class="c2"><a name="h.7qzdi2w8gp73"></a><span>Test Digestion </span></p><p class="c1"><a name="h.9ijopada3shk"></a></p><a href="#" name="729b1547a7bce2086e0405f840e06e2d6f2617fc"></a><a href="#" name="41"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c73"><p class="c1 c4"><a name="h.ho1jt6iq2h8k"></a></p></td><td class="c73"><p class="c4 c2"><a name="h.pkf1jsm450zm"></a><span>i5-30-1</span></p></td><td class="c73"><p class="c4 c2"><a name="h.7el0px6le1a7"></a><span>i5-30-5</span></p></td><td class="c73"><p class="c4 c2"><a name="h.24ib2ir6m2ox"></a><span>i5-30-7</span></p></td><td class="c73"><p class="c4 c2"><a name="h.u9z7nr8b8j0l"></a><span>i5-30-8</span></p></td><td class="c73"><p class="c4 c2"><a name="h.jd3f1732uftp"></a><span>i5-30-9</span></p></td></tr><tr class="c8"><td class="c73"><p class="c4 c2"><a name="h.hjif9nafr6s5"></a><span>DNA</span></p></td><td class="c73"><p class="c4 c2"><a name="h.b8195jnfhmyt"></a><span>4</span></p></td><td class="c73"><p class="c4 c2"><a name="h.g2vpeq68qpjz"></a><span>3</span></p></td><td class="c73"><p class="c4 c2"><a name="h.tlcg0csj8q9o"></a><span>4</span></p></td><td class="c73"><p class="c4 c2"><a name="h.vtyhzhp29j1s"></a><span>3</span></p></td><td class="c73"><p class="c4 c2"><a name="h.n4fls87mzdah"></a><span>2</span></p></td></tr><tr class="c8"><td class="c73"><p class="c4 c2"><a name="h.ta72xr1vgm6g"></a><span>Buffer</span></p></td><td class="c73"><p class="c4 c2"><a name="h.6pfp49qxzz2z"></a><span>2</span></p></td><td class="c73"><p class="c4 c2"><a name="h.an7yur76434y"></a><span>2</span></p></td><td class="c73"><p class="c4 c2"><a name="h.bmnsoogx6kls"></a><span>2</span></p></td><td class="c73"><p class="c4 c2"><a name="h.4m15zdyya5rb"></a><span>2</span></p></td><td class="c73"><p class="c4 c2"><a name="h.jg1wwznu9h7i"></a><span>2</span></p></td></tr><tr class="c8"><td class="c73"><p class="c4 c2"><a name="h.hbxgu9ja18rw"></a><span>EcoRI</span></p></td><td class="c73"><p class="c4 c2"><a name="h.k4z0oqwlyqpk"></a><span>1</span></p></td><td class="c73"><p class="c4 c2"><a name="h.b8wnd3bwqon6"></a><span>1</span></p></td><td class="c73"><p class="c4 c2"><a name="h.548nat6b9w6j"></a><span>1</span></p></td><td class="c73"><p class="c4 c2"><a name="h.8i4o4jvawxr1"></a><span>1</span></p></td><td class="c73"><p class="c4 c2"><a name="h.ogg33qbohcgv"></a><span>1</span></p></td></tr><tr class="c8"><td class="c73"><p class="c4 c2"><a name="h.5eqsi863qd7m"></a><span>PstI</span></p></td><td class="c73"><p class="c4 c2"><a name="h.9ie5iuta43y9"></a><span>1</span></p></td><td class="c73"><p class="c4 c2"><a name="h.ip7ekaxu5f2r"></a><span>1</span></p></td><td class="c73"><p class="c4 c2"><a name="h.1p411tww0gjc"></a><span>1</span></p></td><td class="c73"><p class="c4 c2"><a name="h.af9dcgo9r8lg"></a><span>1</span></p></td><td class="c73"><p class="c4 c2"><a name="h.8slr5xs1f1d"></a><span>1</span></p></td></tr><tr class="c8"><td class="c73"><p class="c4 c2"><a name="h.7svg6bh9t6le"></a><span>Water</span></p></td><td class="c73"><p class="c4 c2"><a name="h.5mtz6i934nq2"></a><span>12</span></p></td><td class="c73"><p class="c4 c2"><a name="h.mdut81am6251"></a><span>13</span></p></td><td class="c73"><p class="c4 c2"><a name="h.qfgs3ysmz2q9"></a><span>12</span></p></td><td class="c73"><p class="c4 c2"><a name="h.6r9hcb7b6zdq"></a><span>13</span></p></td><td class="c73"><p class="c4 c2"><a name="h.2pj0e6fg6xdk"></a><span>14</span></p></td></tr><tr class="c8"><td class="c73"><p class="c4 c2"><a name="h.h4hom3e1ujw3"></a><span>Total</span></p></td><td class="c73"><p class="c4 c2"><a name="h.4706ttpwek2z"></a><span>20</span></p></td><td class="c73"><p class="c4 c2"><a name="h.ml9hn9pp61rx"></a><span>20</span></p></td><td class="c73"><p class="c4 c2"><a name="h.k8veicqw4kv"></a><span>20</span></p></td><td class="c73"><p class="c4 c2"><a name="h.apq0moj322ez"></a><span>20</span></p></td><td class="c73"><p class="c4 c2"><a name="h.rxse6jl2tsge"></a><span>20</span></p></td></tr></tbody></table><p class="c1"><a name="h.ygicqrth68qz"></a></p><p class="c2"><a name="h.8hp55y1g01wx"></a><span>8/13</span></p><p class="c2"><a name="h.yx6z65elmr5b"></a><span>Repeat Digestion of CP medium from 8/8 </span></p><p class="c1"><a name="h.bwm7rqkds14m"></a></p><a href="#" name="a476edab86376cacb30105a760f9e0bc715b499a"></a><a href="#" name="42"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c79"><p class="c1 c4"><a name="h.dv5qx0favqqa"></a></p></td><td class="c83"><p class="c4 c2"><a name="h.nz82xc7e01g6"></a><span>i5-25-7</span></p></td></tr><tr class="c8"><td class="c79"><p class="c4 c2"><a name="h.7vv70o4k78pk"></a><span>DNA</span></p></td><td class="c83"><p class="c4 c2"><a name="h.lgu14qf36qfo"></a><span>20</span></p></td></tr><tr class="c8"><td class="c79"><p class="c4 c2"><a name="h.u1swwb9q8awq"></a><span>Buffer</span></p></td><td class="c83"><p class="c4 c2"><a name="h.5i7qfzvnj3pl"></a><span>5</span></p></td></tr><tr class="c8"><td class="c79"><p class="c4 c2"><a name="h.qrzhx6mr6wwb"></a><span>PstI</span></p></td><td class="c83"><p class="c4 c2"><a name="h.1088ioza8a33"></a><span>1</span></p></td></tr><tr class="c8"><td class="c79"><p class="c4 c2"><a name="h.9p92448b6d1q"></a><span>SpcI</span></p></td><td class="c83"><p class="c4 c2"><a name="h.gv2zsmjthhee"></a><span>1</span></p></td></tr><tr class="c8"><td class="c79"><p class="c4 c2"><a name="h.d132mkn7dsri"></a><span>Water</span></p></td><td class="c83"><p class="c4 c2"><a name="h.qewdkta6kgva"></a><span>13</span></p></td></tr><tr class="c8"><td class="c79"><p class="c4 c2"><a name="h.huyfpddhtgtt"></a><span>Total</span></p></td><td class="c83"><p class="c4 c2"><a name="h.sfc9bdbspbdo"></a><span>50</span></p></td></tr></tbody></table><p class="c2"><a name="h.q07fj8aatkj8"></a><span>                        → i5-32-1 13.1 ng/ul</span></p><p class="c1"><a name="h.83a11ejiq1bl"></a></p><p class="c2"><a name="h.h0fq67pbrtou"></a><span>Ligation </span></p><p class="c1"><a name="h.zb8ws8jzfrz3"></a></p><a href="#" name="f7921980145d7b0a2a6dc1f0e92ecc8cfbba7c5a"></a><a href="#" name="43"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c53"><p class="c1 c4"><a name="h.x1caggew13f2"></a></p></td><td class="c80"><p class="c4 c2"><a name="h.sdc6rgdv6jlk"></a><span>Insert: i5-26-8 [Cra]</span></p></td><td class="c80"><p class="c4 c2"><a name="h.ow488913hovq"></a><span>Vector: i5-32-1 [CP]</span></p></td></tr><tr class="c8"><td class="c53"><p class="c1 c4"><a name="h.9hn0spu7m8ih"></a></p></td><td class="c80"><p class="c4 c2"><a name="h.s5a78zhhvqcc"></a><span>3:1</span></p></td><td class="c80"><p class="c4 c2"><a name="h.760n4t6ht1gc"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c53"><p class="c4 c2"><a name="h.9hn0spu7m8ih"></a><span>insert</span></p></td><td class="c80"><p class="c4 c2"><a name="h.xw2xpb6fx4ou"></a><span>4.36</span></p></td><td class="c80"><p class="c4 c2"><a name="h.olxy8kdv8cnu"></a><span>0</span></p></td></tr><tr class="c8"><td class="c53"><p class="c4 c2"><a name="h.93kp6ku0iiwh"></a><span>Vector</span></p></td><td class="c80"><p class="c4 c2"><a name="h.rcvg4ygk32zo"></a><span>3.8</span></p></td><td class="c80"><p class="c4 c2"><a name="h.5wmx0igowyu6"></a><span>3.8</span></p></td></tr><tr class="c8"><td class="c53"><p class="c4 c2"><a name="h.1wsohk9s5r10"></a><span>Buffer</span></p></td><td class="c80"><p class="c4 c2"><a name="h.rfm565dvbc4t"></a><span>2</span></p></td><td class="c80"><p class="c4 c2"><a name="h.rvag37m6puj4"></a><span>2</span></p></td></tr><tr class="c8"><td class="c53"><p class="c4 c2"><a name="h.pmalz0lmrokt"></a><span>ligase</span></p></td><td class="c80"><p class="c4 c2"><a name="h.pwctjlc04whd"></a><span>1</span></p></td><td class="c80"><p class="c4 c2"><a name="h.f9a1d51d1dmr"></a><span>1</span></p></td></tr><tr class="c8"><td class="c53"><p class="c4 c2"><a name="h.qa6yiisdvkdk"></a><span>Water</span></p></td><td class="c80"><p class="c4 c2"><a name="h.qk6vu2naq0r6"></a><span>8.8</span></p></td><td class="c80"><p class="c4 c2"><a name="h.yqm7u1tkmdtd"></a><span>13.2</span></p></td></tr><tr class="c8"><td class="c53"><p class="c4 c2"><a name="h.a3clo8qmlbds"></a><span>total</span></p></td><td class="c80"><p class="c4 c2"><a name="h.z59eeer6r1se"></a><span>20</span></p></td><td class="c80"><p class="c4 c2"><a name="h.5rvon3dlqqxt"></a><span>20</span></p></td></tr><tr class="c8"><td class="c53"><p class="c1 c4"><a name="h.vn9sjwmsj6xx"></a></p></td><td class="c80"><p class="c4 c2"><a name="h.jsrsefbvapq8"></a><span>1</span></p></td><td class="c80"><p class="c4 c2"><a name="h.ag5akp7a4jps"></a><span>2</span></p></td></tr></tbody></table><p class="c1"><a name="h.1tvhctn67d92"></a></p><p class="c1"><a name="h.qzwgdl1ntt7d"></a></p><a href="#" name="2a6e9d48bb41b4c2480d25a13c187b1c87c3892a"></a><a href="#" name="44"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c62"><p class="c1 c4"><a name="h.ipdc1yd24vxf"></a></p></td><td class="c103"><p class="c4 c2"><a name="h.f4wjlhm56vu7"></a><span>Insert: i5-26-9 [Scr]</span></p></td><td class="c100"><p class="c4 c2"><a name="h.f4wjlhm56vu7"></a><span>Vector: i5-31-1 [CP]</span></p></td></tr><tr class="c8"><td class="c62"><p class="c1 c4"><a name="h.qh1y06b3479c"></a></p></td><td class="c103"><p class="c4 c2"><a name="h.48wssfx7r1u7"></a><span>3:1</span></p></td><td class="c100"><p class="c4 c2"><a name="h.48wssfx7r1u7"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c62"><p class="c4 c2"><a name="h.9hn0spu7m8ih"></a><span>insert</span></p></td><td class="c103"><p class="c4 c2"><a name="h.b11zavitsha"></a><span>5.13</span></p></td><td class="c100"><p class="c4 c2"><a name="h.b11zavitsha"></a><span>0</span></p></td></tr><tr class="c8"><td class="c62"><p class="c4 c2"><a name="h.93kp6ku0iiwh"></a><span>Vector</span></p></td><td class="c103"><p class="c4 c2"><a name="h.uq46wo93oxow"></a><span>3.8</span></p></td><td class="c100"><p class="c4 c2"><a name="h.uq46wo93oxow"></a><span>3.8</span></p></td></tr><tr class="c8"><td class="c62"><p class="c4 c2"><a name="h.1wsohk9s5r10"></a><span>Buffer</span></p></td><td class="c103"><p class="c4 c2"><a name="h.ivm0seypj81u"></a><span>2</span></p></td><td class="c100"><p class="c4 c2"><a name="h.ivm0seypj81u"></a><span>2</span></p></td></tr><tr class="c8"><td class="c62"><p class="c4 c2"><a name="h.pmalz0lmrokt"></a><span>ligase</span></p></td><td class="c103"><p class="c4 c2"><a name="h.b4bsa7b2vnai"></a><span>1</span></p></td><td class="c100"><p class="c4 c2"><a name="h.b4bsa7b2vnai"></a><span>1</span></p></td></tr><tr class="c8"><td class="c62"><p class="c4 c2"><a name="h.qa6yiisdvkdk"></a><span>Water</span></p></td><td class="c103"><p class="c4 c2"><a name="h.28sx6m74nth0"></a><span>8</span></p></td><td class="c100"><p class="c4 c2"><a name="h.28sx6m74nth0"></a><span>13.2</span></p></td></tr><tr class="c8"><td class="c62"><p class="c4 c2"><a name="h.a3clo8qmlbds"></a><span>total</span></p></td><td class="c103"><p class="c4 c2"><a name="h.pqek5guz4619"></a><span>20</span></p></td><td class="c100"><p class="c4 c2"><a name="h.pqek5guz4619"></a><span>20</span></p></td></tr><tr class="c8"><td class="c62"><p class="c1 c4"><a name="h.ebecajg2zi5f"></a></p></td><td class="c103"><p class="c4 c2"><a name="h.bzw6gpt1ozvt"></a><span>3</span></p></td><td class="c100"><p class="c4 c2"><a name="h.bzw6gpt1ozvt"></a><span>4</span></p></td></tr></tbody></table><p class="c1"><a name="h.vf0fvkdmnzqx"></a></p><p class="c2"><a name="h.q9a8up4ldyhk"></a><span>Transformation </span></p><p class="c2"><a name="h.arfeyr3nrwf1"></a><span>1 → i5-33-1 → 0 colonies </span></p><p class="c2"><a name="h.7j7e2d5zvfnv"></a><span>2 → i5-33-2 → 0 colonies </span></p><p class="c2"><a name="h.tj6n93n3hd83"></a><span>3 → i5-33-3 → 0 colonies </span></p><p class="c2"><a name="h.lj3xj5v1afoj"></a><span>4→ i5-33-4 → 0 colonies </span></p><p class="c2"><a name="h.mqacxtcn19jp"></a><span>Positive Control (i5-22-2) → 0 colonies </span></p><p class="c2"><a name="h.2d1ug8400cez"></a><span>Retransformation of strong CP fro 8/16 → i5-33-5 → 50 + colonies picked 2 colonies → i5-33-6</span></p><p class="c2"><a name="h.m5cstemmlke7"></a><span>                                                                                        i5-33-7</span></p><p class="c1"><a name="h.dk6cgw8yaj3l"></a></p><p class="c2"><a name="h.qw529owinve4"></a><span>8/14 </span></p><p class="c2"><a name="h.6s52vkatmbqc"></a><span>Made Glycerol stock of:</span></p><p class="c2"><a name="h.733nppqgji5e"></a><span>Dex (i5-33-8)</span></p><p class="c1"><a name="h.tupe8rjtea8j"></a></p><p class="c2"><a name="h.2n5f35kjdexk"></a><span>Miniprep/ Glycerol stock </span></p><p class="c2"><a name="h.56b95g7u2c82"></a><span>i5-33-8 Dex → 154</span></p><p class="c2"><a name="h.l505z58uct98"></a><span>i5-33-9 Com A-1 → 81.8</span></p><p class="c2"><a name="h.woxm5mh4s8yx"></a><span>i5-33-10 Com D-1 → 70.5</span></p><p class="c2"><a name="h.7dy67k57t83u"></a><span>i5-33-11 Com E-1 → 97.7</span></p><p class="c2"><a name="h.j67czes1nje3"></a><span>i5-33-12 Com A-2 → 77.6</span></p><p class="c2"><a name="h.8o82cq131mwq"></a><span>i5-33-13 Com D-2 → 47.3</span></p><p class="c2"><a name="h.nhsd22vcnxzk"></a><span>i5-33-14 Com E-2 → 127. 5</span></p><p class="c1"><a name="h.6qqlcwmj894y"></a></p><p class="c2"><a name="h.1vzgbdfgusl"></a><span>Transformation redo from 8/13</span></p><p class="c2"><a name="h.d9xabqe163og"></a><span>1 → i5-34-1</span></p><p class="c2"><a name="h.ajqqufir100x"></a><span>2 → i5-34-2</span></p><p class="c2"><a name="h.mala9ohzl2"></a><span>3→ i5-34-3         [All colonies counts next page]</span></p><p class="c2"><a name="h.5u9mnvrjowzf"></a><span>4→ i5-34-4</span></p><p class="c2"><a name="h.7lp05xi8o5jz"></a><span>i5-10-1 → positive control</span></p><p class="c1"><a name="h.20jfauarvdlp"></a></p><p class="c2"><a name="h.owy03xy20j5h"></a><span>Test Digestion </span></p><p class="c1"><a name="h.f6q2bhpdazf5"></a></p><a href="#" name="763ae82b5bad88d9b75907a8b6179cfa0b4e3ecb"></a><a href="#" name="45"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c31"><p class="c1 c4"><a name="h.lxz4mxrdunih"></a></p></td><td class="c11"><p class="c4 c2"><a name="h.v9xpuald20wk"></a><span>i5-33-8</span></p></td></tr><tr class="c8"><td class="c31"><p class="c4 c2"><a name="h.ci3rk05ry5ry"></a><span>DNA</span></p></td><td class="c11"><p class="c4 c2"><a name="h.x9gcwu5g13mr"></a><span>2</span></p></td></tr><tr class="c8"><td class="c31"><p class="c4 c2"><a name="h.36xpyj93xpyr"></a><span>Cutsmart</span></p></td><td class="c11"><p class="c4 c2"><a name="h.70xva2p118jk"></a><span>2</span></p></td></tr><tr class="c8"><td class="c31"><p class="c4 c2"><a name="h.n2y7cvs0db2t"></a><span>EcoRI</span></p></td><td class="c11"><p class="c4 c2"><a name="h.j3mb0eg4shbn"></a><span>1</span></p></td></tr><tr class="c8"><td class="c31"><p class="c4 c2"><a name="h.qq9rejw4pt8y"></a><span>PstI</span></p></td><td class="c11"><p class="c4 c2"><a name="h.f2q0gtusxx5h"></a><span>1</span></p></td></tr><tr class="c8"><td class="c31"><p class="c4 c2"><a name="h.ru7ua7fjbnn"></a><span>Water</span></p></td><td class="c11"><p class="c4 c2"><a name="h.7gvq28yviv4r"></a><span>14</span></p></td></tr><tr class="c8"><td class="c31"><p class="c4 c2"><a name="h.c2bhr457dozj"></a><span>Total</span></p></td><td class="c11"><p class="c4 c2"><a name="h.dzzcigrpxzz9"></a><span>20</span></p></td></tr></tbody></table><p class="c1"><a name="h.9bt0nbe8x9jf"></a></p><p class="c2"><a name="h.vl55fgfq33t8"></a><span>Test Digestion </span></p><p class="c1"><a name="h.us9lwo4derb9"></a></p><a href="#" name="8e88e4342ef8671748c05701d136be4b2862588e"></a><a href="#" name="46"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c13"><p class="c1 c4"><a name="h.dzj8kdmrw064"></a></p></td><td class="c13"><p class="c4 c2"><a name="h.fx2prjlkq76j"></a><span>i5-33-9</span></p></td><td class="c13"><p class="c4 c2"><a name="h.7ht2krvpioy7"></a><span>i5-33-10</span></p></td><td class="c13"><p class="c4 c2"><a name="h.my0t2hlhzat1"></a><span>i5-33-11</span></p></td><td class="c13"><p class="c4 c2"><a name="h.93llo9118vsg"></a><span>i5-33-12</span></p></td><td class="c13"><p class="c4 c2"><a name="h.w0foxt8aex4d"></a><span>i5-33-13</span></p></td><td class="c13"><p class="c4 c2"><a name="h.fd58o8cixmp9"></a><span>i5-33-14</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.8t01v6p6g6oc"></a><span>DNA</span></p></td><td class="c13"><p class="c4 c2"><a name="h.n60btcbhfv6l"></a><span>3</span></p></td><td class="c13"><p class="c4 c2"><a name="h.56u2amgcing2"></a><span>3</span></p></td><td class="c13"><p class="c4 c2"><a name="h.uweeq9qxi7z5"></a><span>3</span></p></td><td class="c13"><p class="c4 c2"><a name="h.dhva6bj8wovx"></a><span>3</span></p></td><td class="c13"><p class="c4 c2"><a name="h.i0w5nlihhkey"></a><span>3</span></p></td><td class="c13"><p class="c4 c2"><a name="h.akr7qbkgb7rs"></a><span>3</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.1o0slz60o70b"></a><span>Cutsmart</span></p></td><td class="c13"><p class="c4 c2"><a name="h.diuyuwnqt0t0"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.jzdj4uaceps"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.591d5yr5feug"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.yym2agfwpqlm"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.l4b28dk2yyp4"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.hfxdq2uysvok"></a><span>2</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.kabmxhl1q7p5"></a><span>EcoRI</span></p></td><td class="c13"><p class="c4 c2"><a name="h.g5a1h31nsq8z"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.t0amuaxmmile"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.fk9ozzb1qlgo"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.fvctb7h3kyk6"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.2b5qhqv78tp1"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.crm4mgms332k"></a><span>1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.kabmxhl1q7p5"></a><span>PstI</span></p></td><td class="c13"><p class="c4 c2"><a name="h.g5a1h31nsq8z"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.t0amuaxmmile"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.fk9ozzb1qlgo"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.fvctb7h3kyk6"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.2b5qhqv78tp1"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.crm4mgms332k"></a><span>1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.5hmye17dbm75"></a><span>Water</span></p></td><td class="c13"><p class="c4 c2"><a name="h.yc85jwx5iojg"></a><span>13</span></p></td><td class="c13"><p class="c4 c2"><a name="h.bdtwpekf68tl"></a><span>13</span></p></td><td class="c13"><p class="c4 c2"><a name="h.z1er9hn2id7m"></a><span>13</span></p></td><td class="c13"><p class="c4 c2"><a name="h.405i5cnqv9jx"></a><span>13</span></p></td><td class="c13"><p class="c4 c2"><a name="h.q31bwpoozg1v"></a><span>13</span></p></td><td class="c13"><p class="c4 c2"><a name="h.bwp6ypriegfa"></a><span>13</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.z79365d72210"></a><span>Total </span></p></td><td class="c13"><p class="c4 c2"><a name="h.x1isskclwh35"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.p8rtm5wyi0xu"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.hu038w9qqu68"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.7tr7u75nb19w"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.4reavinajn18"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.f1ewrk9uxa8h"></a><span>20</span></p></td></tr></tbody></table><p class="c2"><a name="h.goheppc2dx61"></a><span>Results: inconclusive</span></p><p class="c1"><a name="h.ml1ph92etfaa"></a></p><p class="c2"><a name="h.si5hs73lcmbi"></a><span>Repeat Test Digestion </span></p><p class="c1"><a name="h.q0m4prd27fxl"></a></p><a href="#" name="cf0002eb5efaacd136af2d718bc509f859fa78d2"></a><a href="#" name="47"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c79"><p class="c1 c4"><a name="h.ldjmfp5uvq51"></a></p></td><td class="c34"><p class="c4 c2"><a name="h.jj7751sgt03e"></a><span>i5-33-8</span></p></td></tr><tr class="c8"><td class="c79"><p class="c4 c2"><a name="h.r5ck60k1i6yt"></a><span>DNA</span></p></td><td class="c34"><p class="c4 c2"><a name="h.nit2z0jd4st"></a><span>2</span></p></td></tr><tr class="c8"><td class="c79"><p class="c4 c2"><a name="h.b079rgaxf3ng"></a><span>Cutsmart</span></p></td><td class="c34"><p class="c4 c2"><a name="h.ufdry88xjj1e"></a><span>2</span></p></td></tr><tr class="c8"><td class="c79"><p class="c4 c2"><a name="h.gnrr87dmenoa"></a><span>SpcI</span></p></td><td class="c34"><p class="c4 c2"><a name="h.8x3hmh3on29p"></a><span>1</span></p></td></tr><tr class="c8"><td class="c79"><p class="c4 c2"><a name="h.c8qhrq7nxqpl"></a><span>XbaI</span></p></td><td class="c34"><p class="c4 c2"><a name="h.d7r04bsp65ij"></a><span>1</span></p></td></tr><tr class="c8"><td class="c79"><p class="c4 c2"><a name="h.754zetd4eibj"></a><span>Water</span></p></td><td class="c34"><p class="c4 c2"><a name="h.ea765ldv4x3v"></a><span>14</span></p></td></tr><tr class="c8"><td class="c79"><p class="c4 c2"><a name="h.so7akcxwhrno"></a><span>Total</span></p></td><td class="c34"><p class="c4 c2"><a name="h.l0rch97n5392"></a><span>20</span></p></td></tr></tbody></table><p class="c2"><a name="h.a89iq8grwvpc"></a><span>Failed</span></p><p class="c1"><a name="h.aft4hmllxw6"></a></p><p class="c2"><a name="h.1fl9kmdwiq0s"></a><span>8/15</span></p><p class="c2"><a name="h.9pnxeap1km09"></a><span>i5-34-1 →  100+ colonies → picked 2 colonies → i5-35-5 [CP(M)-Cra]</span></p><p class="c2"><a name="h.h8ns16axtbxy"></a><span>                                                i5-35-6</span></p><p class="c2"><a name="h.rnj1u24eoh1n"></a><span>i5-34-2 → 0 colonies</span></p><p class="c2"><a name="h.hwzvnqy1v49y"></a><span>i5-34-3 → 50 + colonies → picked 2 colonies → i5-35-3 [CP(M)-Scr]</span></p><p class="c2"><a name="h.v0bhzs2t7dg1"></a><span>                                                i5-35-4</span></p><p class="c2"><a name="h.o6k2epid3y1m"></a><span>i5-34-4 → 2 colonies</span></p><p class="c2"><a name="h.lqkxekytz533"></a><span>+ control → 0 colonies</span></p><p class="c1"><a name="h.ddn15or0pvjt"></a></p><p class="c2"><a name="h.eb99bx8tsmo"></a><span>Miniprep And Glycerol Stock made</span></p><p class="c2"><a name="h.wgkh4hqdjib9"></a><span>i5-33-6 → 53.1 ng/ul        [CP strong]</span></p><p class="c2"><a name="h.2l9z0z37ujxp"></a><span>i5-33-7 → 58.9 ng/ul        [CP strong]</span></p><p class="c1"><a name="h.jbiobo9ztdl3"></a></p><p class="c2"><a name="h.15mzsqz3n0ly"></a><span>Digestion </span></p><p class="c1"><a name="h.wiyqpncv0j5"></a></p><a href="#" name="05dc6e69edb1189742c67d82839f72d087945dae"></a><a href="#" name="48"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c99"><td class="c57"><p class="c1 c4"><a name="h.bdgsnrytul6"></a></p></td><td class="c19"><p class="c4 c2"><a name="h.w9z5jrg1x57h"></a><span>i5-33-6 (CP strong)</span></p></td></tr><tr class="c8"><td class="c57"><p class="c4 c2"><a name="h.b0c0fmtlkt3f"></a><span>DNA</span></p></td><td class="c19"><p class="c4 c2"><a name="h.91w4r2cdnt0t"></a><span>30</span></p></td></tr><tr class="c8"><td class="c57"><p class="c4 c2"><a name="h.yig94fpjns1e"></a><span>NEBuffer</span></p></td><td class="c19"><p class="c4 c2"><a name="h.sr5eiumlgmbu"></a><span>5</span></p></td></tr><tr class="c8"><td class="c57"><p class="c4 c2"><a name="h.9ltstaxfarc7"></a><span>PstI</span></p></td><td class="c19"><p class="c4 c2"><a name="h.s73qz9hfistn"></a><span>1</span></p></td></tr><tr class="c8"><td class="c57"><p class="c4 c2"><a name="h.u5ilp2lcp5bj"></a><span>SpeI</span></p></td><td class="c19"><p class="c4 c2"><a name="h.wuokcmecm27d"></a><span>1</span></p></td></tr><tr class="c8"><td class="c57"><p class="c4 c2"><a name="h.ngg479drtbhh"></a><span>Water</span></p></td><td class="c19"><p class="c4 c2"><a name="h.67e3o1g0j5jj"></a><span>13</span></p></td></tr><tr class="c8"><td class="c57"><p class="c4 c2"><a name="h.gbd3s8pck0vp"></a><span>Total</span></p></td><td class="c19"><p class="c4 c2"><a name="h.3bk31q29c9gh"></a><span>50</span></p></td></tr></tbody></table><p class="c2"><a name="h.d7zb8v4im8lz"></a><span>        i5-35-1 → 13.9 ng/ul </span></p><p class="c1"><a name="h.8v3us1gmtd5t"></a></p><a href="#" name="c7601e78722b9028483841b806f3352b315864f3"></a><a href="#" name="49"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c14"><p class="c1 c4"><a name="h.6fxhopel4kth"></a></p></td><td class="c19"><p class="c4 c2"><a name="h.thvwwy6o83wu"></a><span>i5-10-1 (RBS-GFP)</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.lri2snfhcour"></a><span>DNA</span></p></td><td class="c19"><p class="c4 c2"><a name="h.hdc1walngwa5"></a><span>20</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.js8kzetr99dk"></a><span>NEBuffer</span></p></td><td class="c19"><p class="c4 c2"><a name="h.yw9l8h6wfus8"></a><span>5</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.wxr4meh0e6gj"></a><span>PstI</span></p></td><td class="c19"><p class="c4 c2"><a name="h.l6mmbvsgibh8"></a><span>1</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.ounargsrp2qk"></a><span>XbaI</span></p></td><td class="c19"><p class="c4 c2"><a name="h.hez37ql5nv4b"></a><span>1</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.grnbmt70wwmu"></a><span>Water</span></p></td><td class="c19"><p class="c4 c2"><a name="h.s8i2ruautrt4"></a><span>23</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.mhmxk7kbzx91"></a><span>Total</span></p></td><td class="c19"><p class="c4 c2"><a name="h.e8vmy2mwh768"></a><span>50</span></p></td></tr></tbody></table><p class="c2"><a name="h.e9bxlwesm3h9"></a><span>        i5-35-2 → 17.1 ng/ul </span></p><p class="c1"><a name="h.91z0p3x2ca36"></a></p><p class="c2"><a name="h.37hiui2t0eqr"></a><span>PCR</span></p><p class="c1"><a name="h.v3tapuz1tbpk"></a></p><a href="#" name="9a5b80d5d6e672c917e435dbf711ed639d188cef"></a><a href="#" name="50"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c6"><p class="c4 c2"><a name="h.luigpv11kti0"></a><span>Primers</span></p></td><td class="c6"><p class="c4 c2"><a name="h.i83ocmyj89ou"></a><span>4</span></p></td><td class="c6"><p class="c4 c2"><a name="h.onsriba2lcts"></a><span>10</span></p></td></tr><tr class="c8"><td class="c6"><p class="c4 c2"><a name="h.8ck7zl3t01qq"></a><span>DNA</span></p></td><td class="c6"><p class="c4 c2"><a name="h.vafajfrzu42a"></a><span>1</span></p></td><td class="c6"><p class="c4 c2"><a name="h.d3tpned157iv"></a><span>1</span></p></td></tr><tr class="c8"><td class="c6"><p class="c4 c2"><a name="h.lojiwgflre5e"></a><span>F</span></p></td><td class="c6"><p class="c4 c2"><a name="h.n76arnyhqnaz"></a><span>1</span></p></td><td class="c6"><p class="c4 c2"><a name="h.nv47yawjriya"></a><span>1</span></p></td></tr><tr class="c8"><td class="c6"><p class="c4 c2"><a name="h.ohyt97edpj3r"></a><span>R</span></p></td><td class="c6"><p class="c4 c2"><a name="h.l47l0eaq5ibc"></a><span>1</span></p></td><td class="c6"><p class="c4 c2"><a name="h.ufvoe9hrrvr2"></a><span>1</span></p></td></tr><tr class="c8"><td class="c6"><p class="c4 c2"><a name="h.7q0yr9mz7mxs"></a><span>Phusion </span></p></td><td class="c6"><p class="c4 c2"><a name="h.c0zjhnj8bt96"></a><span>10</span></p></td><td class="c6"><p class="c4 c2"><a name="h.11rmx5egotds"></a><span>10</span></p></td></tr><tr class="c8"><td class="c6"><p class="c4 c2"><a name="h.vfwxkawdm79z"></a><span>Water</span></p></td><td class="c6"><p class="c4 c2"><a name="h.14kzu2xq6ksa"></a><span>7</span></p></td><td class="c6"><p class="c4 c2"><a name="h.vo12j22ezux6"></a><span>7</span></p></td></tr><tr class="c8"><td class="c6"><p class="c4 c2"><a name="h.tp7f6moiu9o7"></a><span>Tota</span></p></td><td class="c6"><p class="c4 c2"><a name="h.vigfadqoy52m"></a><span>20</span></p></td><td class="c6"><p class="c4 c2"><a name="h.pj1g9enicib4"></a><span>20</span></p></td></tr></tbody></table><p class="c1"><a name="h.icv4fyizyb8t"></a></p><p class="c2"><a name="h.e6ztamlui3vf"></a><span>PCR Purification </span></p><p class="c2"><a name="h.tzxi0owohar5"></a><span>1→ 24.4 ng/ul        ComA</span></p><p class="c2"><a name="h.egr67g98m24o"></a><span>2→ 43.6 ng/ul         Dex</span></p><p class="c1"><a name="h.5raa3sfs3ku3"></a></p><p class="c2"><a name="h.nh4olqlh59mv"></a><span>Digestion </span></p><p class="c1"><a name="h.mhfjozi9fu80"></a></p><a href="#" name="9d3ffed307af2dbd498918039490954896a3ee76"></a><a href="#" name="51"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c71"><p class="c1 c4"><a name="h.efof4gy1vxzj"></a></p></td><td class="c73"><p class="c4 c2"><a name="h.1z35g8cwhrxr"></a><span>ComA</span></p></td><td class="c17"><p class="c4 c2"><a name="h.2f6rme6cumya"></a><span>Dex</span></p></td></tr><tr class="c8"><td class="c71"><p class="c4 c2"><a name="h.6w8x1plhemba"></a><span>DNA</span></p></td><td class="c73"><p class="c4 c2"><a name="h.8wptridw7fm"></a><span>30</span></p></td><td class="c17"><p class="c4 c2"><a name="h.v1ye82gh2een"></a><span>30</span></p></td></tr><tr class="c8"><td class="c71"><p class="c4 c2"><a name="h.quisp77awysu"></a><span>Buffer</span></p></td><td class="c73"><p class="c4 c2"><a name="h.f69r8p31sowc"></a><span>5</span></p></td><td class="c17"><p class="c4 c2"><a name="h.cyk3clpfnky7"></a><span>5</span></p></td></tr><tr class="c8"><td class="c71"><p class="c4 c2"><a name="h.x0qk4inxyt7n"></a><span>E</span></p></td><td class="c73"><p class="c4 c2"><a name="h.p2ywfsdycopq"></a><span>1</span></p></td><td class="c17"><p class="c4 c2"><a name="h.ndmxu8jdazmm"></a><span>0</span></p></td></tr><tr class="c8"><td class="c71"><p class="c4 c2"><a name="h.x4glb5qnh54p"></a><span>X</span></p></td><td class="c73"><p class="c4 c2"><a name="h.rpa1pcys26m4"></a><span>0</span></p></td><td class="c17"><p class="c4 c2"><a name="h.8vd9ag7mii8q"></a><span>1</span></p></td></tr><tr class="c8"><td class="c71"><p class="c4 c2"><a name="h.v19rlpfqg53s"></a><span>S</span></p></td><td class="c73"><p class="c4 c2"><a name="h.2cxf3tm6bc"></a><span>1</span></p></td><td class="c17"><p class="c4 c2"><a name="h.me88l1uu0xpd"></a><span>1</span></p></td></tr><tr class="c8"><td class="c71"><p class="c4 c2"><a name="h.x7o6sjj1sxsq"></a><span>Water</span></p></td><td class="c73"><p class="c4 c2"><a name="h.fuj809w7vpkf"></a><span>13</span></p></td><td class="c17"><p class="c4 c2"><a name="h.laffqtto3mjy"></a><span>13</span></p></td></tr><tr class="c8"><td class="c71"><p class="c4 c2"><a name="h.rm77uats1phh"></a><span>Total</span></p></td><td class="c73"><p class="c4 c2"><a name="h.z9ejdad5bi27"></a><span>50</span></p></td><td class="c17"><p class="c4 c2"><a name="h.5qrz284vjn73"></a><span>50</span></p></td></tr></tbody></table><p class="c2"><a name="h.x1e079w76rvn"></a><span>        i5-36-1                        i5-36-2</span></p><p class="c1"><a name="h.2g7aby6b4sja"></a></p><p class="c2"><a name="h.7ikwtgti8hd5"></a><span>Ligation</span></p><p class="c1"><a name="h.2ortfrl8j2sp"></a></p><a href="#" name="1227c9c6f403d6c0a3cacde33274b481d477f1e4"></a><a href="#" name="52"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c77"><p class="c1 c4"><a name="h.446u184cvrbf"></a></p></td><td class="c88"><p class="c4 c2"><a name="h.ja75d7spkaqi"></a><span>Insert: i5-35-2</span></p></td><td class="c41"><p class="c4 c2"><a name="h.k6zu2ge2aals"></a><span>Vector: i5-26-7</span></p></td></tr><tr class="c8"><td class="c77"><p class="c1 c4"><a name="h.ais0p8pbx53e"></a></p></td><td class="c88"><p class="c4 c2"><a name="h.7u0t2140hcem"></a><span>3:1</span></p></td><td class="c41"><p class="c4 c2"><a name="h.61xlumz88osz"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c77"><p class="c4 c2"><a name="h.ais0p8pbx53e"></a><span>Insert</span></p></td><td class="c88"><p class="c4 c2"><a name="h.o2vcyjd38d91"></a><span>3.4</span></p></td><td class="c41"><p class="c4 c2"><a name="h.2fthvm98b3is"></a><span>0</span></p></td></tr><tr class="c8"><td class="c77"><p class="c4 c2"><a name="h.y7nq0i4hddjb"></a><span>Vector</span></p></td><td class="c88"><p class="c4 c2"><a name="h.gr4k6liyrm8f"></a><span>2</span></p></td><td class="c41"><p class="c4 c2"><a name="h.3od12bj6jqka"></a><span>2</span></p></td></tr><tr class="c8"><td class="c77"><p class="c4 c2"><a name="h.7f9fqw9tfe83"></a><span>Buffer</span></p></td><td class="c88"><p class="c4 c2"><a name="h.78rj1ra7fpk7"></a><span>2</span></p></td><td class="c41"><p class="c4 c2"><a name="h.rzwwuegmcbg3"></a><span>2</span></p></td></tr><tr class="c8"><td class="c77"><p class="c4 c2"><a name="h.7bcr7zyep0ao"></a><span>Ligase</span></p></td><td class="c88"><p class="c4 c2"><a name="h.26hp2wrkq5us"></a><span>1</span></p></td><td class="c41"><p class="c4 c2"><a name="h.8mjqylqwwqms"></a><span>1</span></p></td></tr><tr class="c8"><td class="c77"><p class="c4 c2"><a name="h.ou681b2fez89"></a><span>Water</span></p></td><td class="c88"><p class="c4 c2"><a name="h.3m8taamd7nxu"></a><span>11.6</span></p></td><td class="c41"><p class="c4 c2"><a name="h.qpstgtinnx2y"></a><span>15</span></p></td></tr><tr class="c8"><td class="c77"><p class="c4 c2"><a name="h.gxspftcsh4ih"></a><span>Total</span></p></td><td class="c88"><p class="c4 c2"><a name="h.q9oa3rbhng9g"></a><span>20</span></p></td><td class="c41"><p class="c4 c2"><a name="h.kbwez6myde3b"></a><span>20</span></p></td></tr><tr class="c8"><td class="c77"><p class="c1 c4"><a name="h.tbjc9f9wah6k"></a></p></td><td class="c88"><p class="c4 c2"><a name="h.wawvtmqqmgs4"></a><span>1</span></p></td><td class="c41"><p class="c4 c2"><a name="h.eu425i3oyc1y"></a><span>2</span></p></td></tr></tbody></table><p class="c2"><a name="h.rluy16t8b52j"></a><span>        </span></p><a href="#" name="fee9accc571522d9f797ac889a962b7e92f1c9f9"></a><a href="#" name="53"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c59"><p class="c1 c4"><a name="h.7r205nsg42zm"></a></p></td><td class="c24"><p class="c4 c2"><a name="h.3vi5fw695hr3"></a><span>Insert: i5-35-2</span></p></td><td class="c70"><p class="c4 c2"><a name="h.hwa5u13k1iwv"></a><span>Vector: i5-32-1</span></p></td></tr><tr class="c8"><td class="c59"><p class="c1 c4"><a name="h.1bvzbhv2uwc9"></a></p></td><td class="c24"><p class="c4 c2"><a name="h.am7cktusj87g"></a><span>3:1</span></p></td><td class="c70"><p class="c4 c2"><a name="h.18tkuz7w6jlv"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c59"><p class="c4 c2"><a name="h.ais0p8pbx53e"></a><span>Insert</span></p></td><td class="c24"><p class="c4 c2"><a name="h.r26r5wp2h3zy"></a><span>3.4</span></p></td><td class="c70"><p class="c4 c2"><a name="h.av3s0e2o73ag"></a><span>0</span></p></td></tr><tr class="c8"><td class="c59"><p class="c4 c2"><a name="h.y7nq0i4hddjb"></a><span>Vector</span></p></td><td class="c24"><p class="c4 c2"><a name="h.485wyvn50xr1"></a><span>3.8</span></p></td><td class="c70"><p class="c4 c2"><a name="h.rmtoc1k3iwgv"></a><span>3.8</span></p></td></tr><tr class="c8"><td class="c59"><p class="c4 c2"><a name="h.7f9fqw9tfe83"></a><span>Buffer</span></p></td><td class="c24"><p class="c4 c2"><a name="h.f2odavphjx44"></a><span>2</span></p></td><td class="c70"><p class="c4 c2"><a name="h.d8fc3j8vu66d"></a><span>2</span></p></td></tr><tr class="c8"><td class="c59"><p class="c4 c2"><a name="h.7bcr7zyep0ao"></a><span>Ligase</span></p></td><td class="c24"><p class="c4 c2"><a name="h.1jp365pqla93"></a><span>1</span></p></td><td class="c70"><p class="c4 c2"><a name="h.2chpmzvl7gyu"></a><span>1</span></p></td></tr><tr class="c8"><td class="c59"><p class="c4 c2"><a name="h.ou681b2fez89"></a><span>Water</span></p></td><td class="c24"><p class="c4 c2"><a name="h.e2k95lv8a1o6"></a><span>9.8</span></p></td><td class="c70"><p class="c4 c2"><a name="h.e7b88jjs85vg"></a><span>13.2</span></p></td></tr><tr class="c8"><td class="c59"><p class="c4 c2"><a name="h.gxspftcsh4ih"></a><span>Total</span></p></td><td class="c24"><p class="c4 c2"><a name="h.wh7bbpdbgw1u"></a><span>20</span></p></td><td class="c70"><p class="c4 c2"><a name="h.xaqtpwlmq1op"></a><span>20</span></p></td></tr><tr class="c8"><td class="c59"><p class="c1 c4"><a name="h.agdulkfcjm9t"></a></p></td><td class="c24"><p class="c4 c2"><a name="h.4n6g30d55mp"></a><span>3</span></p></td><td class="c70"><p class="c4 c2"><a name="h.6zst6h5r0ejn"></a><span>4</span></p></td></tr></tbody></table><p class="c1"><a name="h.mb93eitj4hsc"></a></p><a href="#" name="386ac964dd8806146fbe6faa982da9292dabbd15"></a><a href="#" name="54"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c59"><p class="c1 c4"><a name="h.nrlnl2uh09mn"></a></p></td><td class="c24"><p class="c4 c2"><a name="h.x49urbrz3psl"></a><span>Insert: i5-35-2</span></p></td><td class="c47"><p class="c4 c2"><a name="h.igj46wb0fylf"></a><span>Vector: i5-35-1</span></p></td></tr><tr class="c8"><td class="c59"><p class="c1 c4"><a name="h.pi287kbft10o"></a></p></td><td class="c24"><p class="c4 c2"><a name="h.1tqf0d29nwo3"></a><span>3:1</span></p></td><td class="c47"><p class="c4 c2"><a name="h.6odrzlxut793"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c59"><p class="c4 c2"><a name="h.ais0p8pbx53e"></a><span>Insert</span></p></td><td class="c24"><p class="c4 c2"><a name="h.dvpf17uz7zb4"></a><span>3.4</span></p></td><td class="c47"><p class="c4 c2"><a name="h.1pwj8dtfqvjf"></a><span>0</span></p></td></tr><tr class="c8"><td class="c59"><p class="c4 c2"><a name="h.y7nq0i4hddjb"></a><span>Vector</span></p></td><td class="c24"><p class="c4 c2"><a name="h.yzrt9o4no5x4"></a><span>3.8</span></p></td><td class="c47"><p class="c4 c2"><a name="h.6j7asswk37rx"></a><span>3.8</span></p></td></tr><tr class="c8"><td class="c59"><p class="c4 c2"><a name="h.7f9fqw9tfe83"></a><span>Buffer</span></p></td><td class="c24"><p class="c4 c2"><a name="h.yobqbto1ibdx"></a><span>2</span></p></td><td class="c47"><p class="c4 c2"><a name="h.tcji8slkvpx0"></a><span>2</span></p></td></tr><tr class="c8"><td class="c59"><p class="c4 c2"><a name="h.7bcr7zyep0ao"></a><span>Ligase</span></p></td><td class="c24"><p class="c4 c2"><a name="h.yd2rnm4y8y12"></a><span>1</span></p></td><td class="c47"><p class="c4 c2"><a name="h.pk7pom6d1u2u"></a><span>1</span></p></td></tr><tr class="c8"><td class="c59"><p class="c4 c2"><a name="h.ou681b2fez89"></a><span>Water</span></p></td><td class="c24"><p class="c4 c2"><a name="h.t0f9rlc21ws"></a><span>9.8</span></p></td><td class="c47"><p class="c4 c2"><a name="h.k9wsifj3hu8y"></a><span>13.2</span></p></td></tr><tr class="c8"><td class="c59"><p class="c4 c2"><a name="h.gxspftcsh4ih"></a><span>Total</span></p></td><td class="c24"><p class="c4 c2"><a name="h.7pomzfuh0526"></a><span>20</span></p></td><td class="c47"><p class="c4 c2"><a name="h.l7pqn4xi6uh1"></a><span>20</span></p></td></tr><tr class="c8"><td class="c59"><p class="c1 c4"><a name="h.enkw485jp5hr"></a></p></td><td class="c24"><p class="c4 c2"><a name="h.khiijy6wy0l"></a><span>5</span></p></td><td class="c47"><p class="c4 c2"><a name="h.a1d7jjrad43w"></a><span>6</span></p></td></tr></tbody></table><p class="c1"><a name="h.f4jk5w90pd9d"></a></p><p class="c2"><a name="h.2blu38ywme91"></a><span>i5-36-2 → 7.2</span></p><p class="c2"><a name="h.efpckwm48ckh"></a><span>i5-36-1 → 5.5</span></p><p class="c1"><a name="h.qclf1lg5nbj3"></a></p><p class="c2"><a name="h.vsm37xfci8as"></a><span>Ligation </span></p><p class="c1"><a name="h.9zvycs9ruwav"></a></p><a href="#" name="b699df3897563c86ceb49c73d01dfb244fb43787"></a><a href="#" name="55"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c35"><p class="c1 c4"><a name="h.dxt9q8ssmb2i"></a></p></td><td class="c75"><p class="c4 c2"><a name="h.frqh55sr2nnq"></a><span>insert: i5-36-1</span></p></td><td class="c38"><p class="c4 c2"><a name="h.ad8d23ff7t2"></a><span>Vector: i5-35-1</span></p></td></tr><tr class="c8"><td class="c35"><p class="c1 c4"><a name="h.7k1j0y4fpakg"></a></p></td><td class="c75"><p class="c4 c2"><a name="h.vef3x2rqx7dv"></a><span>3:1</span></p></td><td class="c38"><p class="c4 c2"><a name="h.dctwbc6t0l85"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c35"><p class="c4 c2"><a name="h.ais0p8pbx53e"></a><span>Insert</span></p></td><td class="c75"><p class="c4 c2"><a name="h.ecvngv16j278"></a><span>14</span></p></td><td class="c38"><p class="c4 c2"><a name="h.um5s8ol7avca"></a><span>0</span></p></td></tr><tr class="c8"><td class="c35"><p class="c4 c2"><a name="h.y7nq0i4hddjb"></a><span>Vector</span></p></td><td class="c75"><p class="c4 c2"><a name="h.8uecg72bbyyh"></a><span>2</span></p></td><td class="c38"><p class="c4 c2"><a name="h.hvlswlaxagkm"></a><span>2</span></p></td></tr><tr class="c8"><td class="c35"><p class="c4 c2"><a name="h.7f9fqw9tfe83"></a><span>Buffer</span></p></td><td class="c75"><p class="c4 c2"><a name="h.tarmcxbd8xv"></a><span>2</span></p></td><td class="c38"><p class="c4 c2"><a name="h.g5t1s4dgbdel"></a><span>2</span></p></td></tr><tr class="c8"><td class="c35"><p class="c4 c2"><a name="h.7bcr7zyep0ao"></a><span>Ligase</span></p></td><td class="c75"><p class="c4 c2"><a name="h.ri21cogkisnp"></a><span>1</span></p></td><td class="c38"><p class="c4 c2"><a name="h.8vvltxqxtq3f"></a><span>1</span></p></td></tr><tr class="c8"><td class="c35"><p class="c4 c2"><a name="h.ou681b2fez89"></a><span>Water</span></p></td><td class="c75"><p class="c4 c2"><a name="h.85juk2himz11"></a><span>1</span></p></td><td class="c38"><p class="c4 c2"><a name="h.qizhmhurhfil"></a><span>15</span></p></td></tr><tr class="c8"><td class="c35"><p class="c4 c2"><a name="h.gxspftcsh4ih"></a><span>Total</span></p></td><td class="c75"><p class="c4 c2"><a name="h.588l61v2wpyy"></a><span>20</span></p></td><td class="c38"><p class="c4 c2"><a name="h.ungk98tufb2i"></a><span>20</span></p></td></tr><tr class="c8"><td class="c35"><p class="c1 c4"><a name="h.gqlikebq8qye"></a></p></td><td class="c75"><p class="c4 c2"><a name="h.wv8bg4a2wc3u"></a><span>1</span></p></td><td class="c38"><p class="c4 c2"><a name="h.heh6q5m96vh0"></a><span>2</span></p></td></tr></tbody></table><p class="c1"><a name="h.z2v87m60062"></a></p><a href="#" name="7a81143f15cd37b423cfe73c5985a4323703642c"></a><a href="#" name="56"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c10"><p class="c1 c4"><a name="h.537owfrpel2k"></a></p></td><td class="c75"><p class="c4 c2"><a name="h.pnjlac6u93rt"></a><span>Insert: i5-36-2</span></p></td><td class="c15"><p class="c4 c2"><a name="h.jlnxtvctx31c"></a><span>Vector: i5-35-2</span></p></td></tr><tr class="c8"><td class="c10"><p class="c1 c4"><a name="h.c2xoe0c2eh1n"></a></p></td><td class="c75"><p class="c4 c2"><a name="h.l9j36qbonyaz"></a><span>3:1</span></p></td><td class="c15"><p class="c4 c2"><a name="h.t5y7pj1zrs9b"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c10"><p class="c4 c2"><a name="h.ais0p8pbx53e"></a><span>Insert</span></p></td><td class="c75"><p class="c4 c2"><a name="h.8tisc44fdrm0"></a><span>11</span></p></td><td class="c15"><p class="c4 c2"><a name="h.2t2o6zak6asp"></a><span>0</span></p></td></tr><tr class="c8"><td class="c10"><p class="c4 c2"><a name="h.y7nq0i4hddjb"></a><span>Vector</span></p></td><td class="c75"><p class="c4 c2"><a name="h.9hbux94l4his"></a><span>2</span></p></td><td class="c15"><p class="c4 c2"><a name="h.u94on5msviiy"></a><span>2</span></p></td></tr><tr class="c8"><td class="c10"><p class="c4 c2"><a name="h.7f9fqw9tfe83"></a><span>Buffer</span></p></td><td class="c75"><p class="c4 c2"><a name="h.86ufhok2o4bg"></a><span>2</span></p></td><td class="c15"><p class="c4 c2"><a name="h.uxhxzp95ufr9"></a><span>2</span></p></td></tr><tr class="c8"><td class="c10"><p class="c4 c2"><a name="h.7bcr7zyep0ao"></a><span>Ligase</span></p></td><td class="c75"><p class="c4 c2"><a name="h.rw15wrbffi7n"></a><span>1</span></p></td><td class="c15"><p class="c4 c2"><a name="h.vj2h9qly4owu"></a><span>1</span></p></td></tr><tr class="c8"><td class="c10"><p class="c4 c2"><a name="h.ou681b2fez89"></a><span>Water</span></p></td><td class="c75"><p class="c4 c2"><a name="h.sqiowcllzkon"></a><span>4</span></p></td><td class="c15"><p class="c4 c2"><a name="h.3kah5vuq02rg"></a><span>15</span></p></td></tr><tr class="c8"><td class="c10"><p class="c4 c2"><a name="h.gxspftcsh4ih"></a><span>Total</span></p></td><td class="c75"><p class="c4 c2"><a name="h.5gjl5p87tzyb"></a><span>20</span></p></td><td class="c15"><p class="c4 c2"><a name="h.g8zq8upbpurr"></a><span>20</span></p></td></tr><tr class="c8"><td class="c10"><p class="c1 c4"><a name="h.grj8cm9qh59r"></a></p></td><td class="c75"><p class="c4 c2"><a name="h.fj6uecrb8n39"></a><span>3</span></p></td><td class="c15"><p class="c4 c2"><a name="h.j8lc1i1cnvyf"></a><span>4</span></p></td></tr></tbody></table><p class="c1"><a name="h.xoy1ifbetzcb"></a></p><p class="c2"><a name="h.wknss8al3bw9"></a><span>Transformation </span></p><p class="c2"><a name="h.i3rp59nff5kj"></a><span>1 → i5-37-1 (3:1 CP weak-GFP) → 50+ colonies → picked 1 colony → i5-37-1</span></p><p class="c2"><a name="h.afr5apqmgh7x"></a><span>2→ i5-37-2 (0:1) → 0 colonies</span></p><p class="c2"><a name="h.6u95ndm7eryf"></a><span>3→ i5-37-3 (3:1 CP medium-GFP) → 20+ colonies → picked 1 colony → i5-37-3</span></p><p class="c2"><a name="h.8tjez9d9wwrq"></a><span>4→ i5-37-4 (0:1) → 0 colonies</span></p><p class="c2"><a name="h.gslcbbx4vbd0"></a><span>5→ i5-37-5 (3:1 CP strong- GFP) → 10 colonies → picked 1 colony → i5-37-5</span></p><p class="c2"><a name="h.nce9o8wtiv9r"></a><span>6→ i5-37-6 (0:1) → 0 colonies </span></p><p class="c2"><a name="h.thw8mfciqpze"></a><span>+ control with GFP (pLac-GFP) i5-11-5 → i5-37-7 → 200+ colonies → picked 1 colony → i5-37-7</span></p><p class="c2"><a name="h.5fzgywok5zsu"></a><span>+ control without GFP (pLac) i5-10-3 → i5-37-8 → 300+ colonies → picked 1 colony → i5-37-8</span></p><p class="c1"><a name="h.ho9zttwlrkrd"></a></p><p class="c2"><a name="h.p7g4wz3741my"></a><span>8/16</span></p><p class="c2"><a name="h.xenm88qj8tr0"></a><span>Miniprep and Glycerol Stock made</span></p><p class="c2"><a name="h.w7e9rh2vqpkl"></a><span>i5-35-5 → 102.4 [CP medium -Cra]</span></p><p class="c2"><a name="h.zcm807cia938"></a><span>i5-35-6 → 96.8 [CP medium -Cra]</span></p><p class="c2"><a name="h.bx69nd5oum52"></a><span>i5-35-3 → 174.1 [CP medium -Sur]</span></p><p class="c2"><a name="h.hnfr01465w8h"></a><span>i5-35-4 → 81.9[CP medium -Sur]</span></p><p class="c1"><a name="h.cjlm4a10dfu3"></a></p><p class="c2"><a name="h.5x45icvqjupb"></a><span>Ligation </span></p><p class="c1"><a name="h.j6vssugbvt7n"></a></p><a href="#" name="5a5e919e87b21cebb29d96bd7724740f71fac1d2"></a><a href="#" name="57"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c21"><p class="c1 c4"><a name="h.480jjn4ddaml"></a></p></td><td class="c41"><p class="c4 c2"><a name="h.kgmh70g2y7xo"></a><span>Insert: i5-36-1</span></p></td><td class="c15"><p class="c4 c2"><a name="h.gsx3jhynwuks"></a><span>Vector: i5-31-1</span></p></td></tr><tr class="c8"><td class="c21"><p class="c1 c4"><a name="h.9nx9wtt0euyr"></a></p></td><td class="c41"><p class="c4 c2"><a name="h.vn8opwiae48j"></a><span>3:1</span></p></td><td class="c15"><p class="c4 c2"><a name="h.fphaff3udskg"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c21"><p class="c4 c2"><a name="h.tvcfje28ne7o"></a><span>Vector</span></p><p class="c4 c2"><a name="h.jjcthnhd65gc"></a><span>Insert</span></p></td><td class="c41"><p class="c4 c2"><a name="h.ue0m7uo64kig"></a><span>2</span></p><p class="c4 c2"><a name="h.6wklvoawyj75"></a><span>14</span></p></td><td class="c15"><p class="c4 c2"><a name="h.4umn0ia7shi"></a><span>2</span></p><p class="c4 c2"><a name="h.lrwxvelm8yr5"></a><span>0</span></p></td></tr><tr class="c8"><td class="c21"><p class="c4 c2"><a name="h.eks6fegflllt"></a><span>Buffer</span></p></td><td class="c41"><p class="c4 c2"><a name="h.c052kfza5hvj"></a><span>2</span></p></td><td class="c15"><p class="c4 c2"><a name="h.lez442oz67q4"></a><span>2</span></p></td></tr><tr class="c8"><td class="c21"><p class="c4 c2"><a name="h.igjte5dsmxv"></a><span>Ligase</span></p></td><td class="c41"><p class="c4 c2"><a name="h.84rlo2sfzavb"></a><span>1</span></p></td><td class="c15"><p class="c4 c2"><a name="h.g66311p9ogb8"></a><span>1</span></p></td></tr><tr class="c8"><td class="c21"><p class="c4 c2"><a name="h.opzit7ttzhgr"></a><span>Water</span></p></td><td class="c41"><p class="c4 c2"><a name="h.sbiii5hnpqbv"></a><span>1</span></p></td><td class="c15"><p class="c4 c2"><a name="h.ykdcw8msca4d"></a><span>15</span></p></td></tr><tr class="c8"><td class="c21"><p class="c4 c2"><a name="h.ln3bvpo676an"></a><span>Total </span></p></td><td class="c41"><p class="c4 c2"><a name="h.ivtvob47w6ky"></a><span>20</span></p></td><td class="c15"><p class="c4 c2"><a name="h.ebihepncp1fn"></a><span>20</span></p></td></tr><tr class="c8"><td class="c21"><p class="c1 c4"><a name="h.l8iinr8zi0m2"></a></p></td><td class="c41"><p class="c4 c2"><a name="h.uf0qm8vb8pk9"></a><span>1</span></p></td><td class="c15"><p class="c4 c2"><a name="h.b2amah8qzdry"></a><span>2</span></p></td></tr></tbody></table><p class="c1"><a name="h.bbkojexwxf70"></a></p><p class="c1"><a name="h.vghxd7c06693"></a></p><a href="#" name="e89e9ab2a79d4b7c0dfc327da0d0533b126297ad"></a><a href="#" name="58"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c21"><p class="c1 c4"><a name="h.x1d44wutcfux"></a></p></td><td class="c70"><p class="c4 c2"><a name="h.p490enb55ood"></a><span>Vector: i5-31-2</span></p></td><td class="c41"><p class="c4 c2"><a name="h.f9s6zwrz984e"></a><span>Insert: i5-36-2</span></p></td></tr><tr class="c8"><td class="c21"><p class="c1 c4"><a name="h.3ydbg5rxcnfw"></a></p></td><td class="c70"><p class="c4 c2"><a name="h.iesw723u5fvk"></a><span>3:1</span></p></td><td class="c41"><p class="c4 c2"><a name="h.v57bmrvyii4f"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c21"><p class="c4 c2"><a name="h.tvcfje28ne7o"></a><span>Vector</span></p></td><td class="c70"><p class="c4 c2"><a name="h.otvnii1tpwj6"></a><span>2</span></p></td><td class="c41"><p class="c4 c2"><a name="h.70mhhah07aza"></a><span>2</span></p></td></tr><tr class="c8"><td class="c21"><p class="c4 c2"><a name="h.eks6fegflllt"></a><span>Insert</span></p></td><td class="c70"><p class="c4 c2"><a name="h.mxdk1e2t3mph"></a><span>11</span></p></td><td class="c41"><p class="c4 c2"><a name="h.yrn8y8ux1ost"></a><span>0</span></p></td></tr><tr class="c8"><td class="c21"><p class="c4 c2"><a name="h.eks6fegflllt"></a><span>Buffer</span></p></td><td class="c70"><p class="c4 c2"><a name="h.nazn83xlqr16"></a><span>2</span></p></td><td class="c41"><p class="c4 c2"><a name="h.1n1692j4zc5f"></a><span>2</span></p></td></tr><tr class="c8"><td class="c21"><p class="c4 c2"><a name="h.igjte5dsmxv"></a><span>Ligase</span></p></td><td class="c70"><p class="c4 c2"><a name="h.gk7uqqggkn8j"></a><span>1</span></p></td><td class="c41"><p class="c4 c2"><a name="h.vw4m32eo9wed"></a><span>1</span></p></td></tr><tr class="c8"><td class="c21"><p class="c4 c2"><a name="h.opzit7ttzhgr"></a><span>Water</span></p></td><td class="c70"><p class="c4 c2"><a name="h.qsebwi85qwlx"></a><span>4</span></p></td><td class="c41"><p class="c4 c2"><a name="h.uuu3n62mo9sq"></a><span>15</span></p></td></tr><tr class="c8"><td class="c21"><p class="c4 c2"><a name="h.ln3bvpo676an"></a><span>Total </span></p></td><td class="c70"><p class="c4 c2"><a name="h.2z45cb58uzve"></a><span>20</span></p></td><td class="c41"><p class="c4 c2"><a name="h.1u63u2j7cbs5"></a><span>20</span></p></td></tr><tr class="c8"><td class="c21"><p class="c1 c4"><a name="h.weyf48fo89s5"></a></p></td><td class="c70"><p class="c4 c2"><a name="h.b393zp9achik"></a><span>3</span></p></td><td class="c41"><p class="c4 c2"><a name="h.x2s8ejdapab3"></a><span>4</span></p></td></tr></tbody></table><p class="c1"><a name="h.79vsjsq0ozaz"></a></p><p class="c2"><a name="h.bck05rrifjsn"></a><span>Digestion</span></p><a href="#" name="da87f34366964cbda2c79064dd7a5557c2a56ccf"></a><a href="#" name="59"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c79"><p class="c1 c4"><a name="h.cp3drxo02zlu"></a></p></td><td class="c35"><p class="c4 c2"><a name="h.9bgajhef44mo"></a><span>i5-35-5</span></p></td><td class="c10"><p class="c4 c2"><a name="h.ilytc521q7z8"></a><span>i5-35-3</span></p></td></tr><tr class="c8"><td class="c79"><p class="c4 c2"><a name="h.ftgy4652e3jj"></a><span>DNA</span></p></td><td class="c35"><p class="c4 c2"><a name="h.4zwquazf9r00"></a><span>20</span></p></td><td class="c10"><p class="c4 c2"><a name="h.5vkb6663ecbc"></a><span>20</span></p></td></tr><tr class="c8"><td class="c79"><p class="c4 c2"><a name="h.cw8ungggm5i2"></a><span>NEBuffer</span></p></td><td class="c35"><p class="c4 c2"><a name="h.n97w1d3ne244"></a><span>5</span></p></td><td class="c10"><p class="c4 c2"><a name="h.6r3hr3ocjrlh"></a><span>5</span></p></td></tr><tr class="c8"><td class="c79"><p class="c4 c2"><a name="h.5cwj9i1t3fn8"></a><span>PstI</span></p></td><td class="c35"><p class="c4 c2"><a name="h.cn1rhr6fozee"></a><span>1</span></p></td><td class="c10"><p class="c4 c2"><a name="h.idpxjh190o22"></a><span>1</span></p></td></tr><tr class="c8"><td class="c79"><p class="c4 c2"><a name="h.drgnwxr8oakf"></a><span>SpeI</span></p></td><td class="c35"><p class="c4 c2"><a name="h.8tw8lhpig6pm"></a><span>1</span></p></td><td class="c10"><p class="c4 c2"><a name="h.p7gjrzcrha61"></a><span>1</span></p></td></tr><tr class="c8"><td class="c79"><p class="c4 c2"><a name="h.t579oqr0nmx4"></a><span>Water</span></p></td><td class="c35"><p class="c4 c2"><a name="h.gelxflpj4r9o"></a><span>13</span></p></td><td class="c10"><p class="c4 c2"><a name="h.rhr78tydnl0v"></a><span>13</span></p></td></tr><tr class="c8"><td class="c79"><p class="c4 c2"><a name="h.16nz6uq6ddrr"></a><span>Total</span></p></td><td class="c35"><p class="c4 c2"><a name="h.622yhn26s54o"></a><span>50</span></p></td><td class="c10"><p class="c4 c2"><a name="h.ywwgyfb4gcot"></a><span>50</span></p></td></tr><tr class="c8"><td class="c79"><p class="c1 c4"><a name="h.5pgdl8fqw7i"></a></p></td><td class="c35"><p class="c4 c2"><a name="h.5httc9x9d6rz"></a><span>i5-38-5</span></p></td><td class="c10"><p class="c4 c2"><a name="h.ybu75mfzznxf"></a><span>i5-38-6</span></p></td></tr></tbody></table><p class="c1"><a name="h.k5jbwb3fp85x"></a></p><p class="c1"><a name="h.xwh3vt5mhwz6"></a></p><a href="#" name="d16ddbe4f852fdb8c9032948186c12b1c32aae69"></a><a href="#" name="60"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c74"><p class="c1 c4"><a name="h.yexdchbtvwmh"></a></p></td><td class="c53"><p class="c4 c2"><a name="h.a0ub1rdwnu0m"></a><span>i5-30-1</span></p></td><td class="c21"><p class="c4 c2"><a name="h.oorpyfrunmgr"></a><span>i5-30-8</span></p></td></tr><tr class="c8"><td class="c74"><p class="c4 c2"><a name="h.ftgy4652e3jj"></a><span>DNA</span></p></td><td class="c53"><p class="c4 c2"><a name="h.xdo1mb5698j4"></a><span>20</span></p></td><td class="c21"><p class="c4 c2"><a name="h.rwx9ye92aef"></a><span>20</span></p></td></tr><tr class="c8"><td class="c74"><p class="c4 c2"><a name="h.cw8ungggm5i2"></a><span>NEBuffer</span></p></td><td class="c53"><p class="c4 c2"><a name="h.8uwpbjiox103"></a><span>5</span></p></td><td class="c21"><p class="c4 c2"><a name="h.y6ti414jt9dj"></a><span>5</span></p></td></tr><tr class="c8"><td class="c74"><p class="c4 c2"><a name="h.5cwj9i1t3fn8"></a><span>PstI</span></p></td><td class="c53"><p class="c4 c2"><a name="h.dtp9b4osul7y"></a><span>1</span></p></td><td class="c21"><p class="c4 c2"><a name="h.b0sf5zoszj33"></a><span>1</span></p></td></tr><tr class="c8"><td class="c74"><p class="c4 c2"><a name="h.drgnwxr8oakf"></a><span>XbaI</span></p></td><td class="c53"><p class="c4 c2"><a name="h.1b0mlm3ivl2e"></a><span>1</span></p></td><td class="c21"><p class="c4 c2"><a name="h.tjl2sgugzhus"></a><span>1</span></p></td></tr><tr class="c8"><td class="c74"><p class="c4 c2"><a name="h.t579oqr0nmx4"></a><span>Water</span></p></td><td class="c53"><p class="c4 c2"><a name="h.lsjz7a77eu04"></a><span>13</span></p></td><td class="c21"><p class="c4 c2"><a name="h.qba6p47y043k"></a><span>13</span></p></td></tr><tr class="c8"><td class="c74"><p class="c4 c2"><a name="h.16nz6uq6ddrr"></a><span>Total</span></p></td><td class="c53"><p class="c4 c2"><a name="h.vpq2fyh1dpf4"></a><span>50 </span></p></td><td class="c21"><p class="c4 c2"><a name="h.y1b7c1a8jlet"></a><span>50</span></p></td></tr><tr class="c8"><td class="c74"><p class="c1 c4"><a name="h.m8tt9g2ks5iq"></a></p></td><td class="c53"><p class="c4 c2"><a name="h.nog0hulwra5m"></a><span>i5-38-7</span></p></td><td class="c21"><p class="c4 c2"><a name="h.bqlxrvbujnv4"></a><span>i5-38-8</span></p></td></tr></tbody></table><p class="c1"><a name="h.8ky659ftgrfe"></a></p><p class="c2"><a name="h.1cho3s998m0m"></a><span>Transformation</span></p><p class="c2"><a name="h.42b7i439vc6k"></a><span>i5-36-1 3:1 → i5-38-1 → 30 colonies → picked 2 colonies →  i5-38-9</span></p><p class="c2"><a name="h.a6yy48ute5r8"></a><span>                                                        i5-38-10</span></p><p class="c2"><a name="h.xsfqvvxh8333"></a><span>i5-36-1 0:1 → i5-38-2 → 8 colonies</span></p><p class="c2"><a name="h.k28xj92cimb8"></a><span>i5-36-2 3:1 → i5-38-3 → 0 colonies</span></p><p class="c2"><a name="h.7fp5osalqr18"></a><span>i5-36-2 0:1 → i5-38-4 → 1 colonies</span></p><p class="c2"><a name="h.iwcul570p9na"></a><span>Control → i5-38-11</span></p><p class="c1"><a name="h.a8qnl3t33nlo"></a></p><p class="c2"><a name="h.c4b87hf8e4u2"></a><span>Digestion</span></p><p class="c2"><a name="h.chyifkx13l2y"></a><span>Digested parts </span></p><p class="c2"><a name="h.e5i57g1il10m"></a><span>i5-38-5 (Backbone- CP-Cra) → 19.0</span></p><p class="c2"><a name="h.wukqvlly8r0c"></a><span>i5-38-6 (Backbone- CP-Scr) → 43.2</span></p><p class="c2"><a name="h.abr9bnvsvkgm"></a><span>i5-38-7 (pFru-RBS-GFP) → 11.3</span></p><p class="c2"><a name="h.daq45zhhkkz5"></a><span>i5-38-8 (pScr-RBS-GFP) → 4.9</span></p><p class="c1"><a name="h.9rw4l13zbphq"></a></p><p class="c2"><a name="h.n2fbxy6ebygf"></a><span>Ligation</span></p><p class="c1"><a name="h.mtbov0uvp4oe"></a></p><a href="#" name="dd5a8cd271d9a457ce7650362cb1f18cd6095058"></a><a href="#" name="61"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c14"><p class="c1 c4"><a name="h.saafwxx92ife"></a></p></td><td class="c55"><p class="c4 c2"><a name="h.zngy8nh8t0c"></a><span>Insert: i5-38-7 (pFru-RBS-GFP)</span></p></td><td class="c64"><p class="c4 c2"><a name="h.b2s1h0zgot4e"></a><span>Vector: i5-38-5 (Backbone-CP-Cra)</span></p></td></tr><tr class="c8"><td class="c14"><p class="c1 c4"><a name="h.larjp1pa1p5r"></a></p></td><td class="c55"><p class="c4 c2"><a name="h.8n8dcb1c3pya"></a><span>3:1</span></p></td><td class="c64"><p class="c4 c2"><a name="h.ila13r6f7fe0"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.d6f2eeurl6w5"></a><span>Insert</span></p></td><td class="c55"><p class="c4 c2"><a name="h.p9twdfyqnnr"></a><span>4.4</span></p></td><td class="c64"><p class="c4 c2"><a name="h.cs1kt2qhih0"></a><span>0</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.1fnje6wmn90k"></a><span>Vector</span></p></td><td class="c55"><p class="c4 c2"><a name="h.vjl1f549c3b6"></a><span>2.6</span></p></td><td class="c64"><p class="c4 c2"><a name="h.3yy0tohq2vkg"></a><span>2.6</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.o986boq4pg56"></a><span>Buffer</span></p></td><td class="c55"><p class="c4 c2"><a name="h.2uxkdcxph2iu"></a><span>2</span></p></td><td class="c64"><p class="c4 c2"><a name="h.xa7jauc842h1"></a><span>2</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.a5dgfsjas4of"></a><span>Ligase</span></p></td><td class="c55"><p class="c4 c2"><a name="h.lj6ftrh52v0l"></a><span>1</span></p></td><td class="c64"><p class="c4 c2"><a name="h.bwajompcq8rn"></a><span>1</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.ez3nqb2i6p2c"></a><span>Water</span></p></td><td class="c55"><p class="c4 c2"><a name="h.yvi76vssdmla"></a><span>10</span></p></td><td class="c64"><p class="c4 c2"><a name="h.4h7kxerze1n8"></a><span>14.4</span></p></td></tr><tr class="c8"><td class="c14"><p class="c4 c2"><a name="h.r8lccqmt9kep"></a><span>Total</span></p></td><td class="c55"><p class="c4 c2"><a name="h.fvmmsj4rq1e9"></a><span>20</span></p></td><td class="c64"><p class="c4 c2"><a name="h.s2ijm1ptkp4j"></a><span>20</span></p></td></tr><tr class="c8"><td class="c14"><p class="c1 c4"><a name="h.ro5oph2r63qm"></a></p></td><td class="c55"><p class="c4 c2"><a name="h.86ekxis5zcy4"></a><span>1</span></p></td><td class="c64"><p class="c4 c2"><a name="h.oi3nbmngom5u"></a><span>2</span></p></td></tr></tbody></table><p class="c1"><a name="h.opedeazge4c3"></a></p><a href="#" name="1f025f77f4d97a10d115c11b553bcfdd96e94039"></a><a href="#" name="62"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c61"><p class="c1 c4"><a name="h.luz9d9u2roo4"></a></p></td><td class="c20"><p class="c4 c2"><a name="h.1mx048cy7a66"></a><span>Insert: i5-38-8 (pScr-RBS-GFP)</span></p></td><td class="c64"><p class="c4 c2"><a name="h.d38pefz839xv"></a><span>Vector: i5-38-6 (Backbone-CP-SCr)</span></p></td></tr><tr class="c8"><td class="c61"><p class="c1 c4"><a name="h.pz8gd921dj79"></a></p></td><td class="c20"><p class="c4 c2"><a name="h.1b0li5mc4w8g"></a><span>3:1</span></p></td><td class="c64"><p class="c4 c2"><a name="h.gu6vcuegktuy"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c61"><p class="c4 c2"><a name="h.d6f2eeurl6w5"></a><span>Insert</span></p></td><td class="c20"><p class="c4 c2"><a name="h.mvxyrz3lem07"></a><span>9.2</span></p></td><td class="c64"><p class="c4 c2"><a name="h.xa9jn6dvh9hn"></a><span>0</span></p></td></tr><tr class="c8"><td class="c61"><p class="c4 c2"><a name="h.1fnje6wmn90k"></a><span>Vector</span></p></td><td class="c20"><p class="c4 c2"><a name="h.hop631q5blju"></a><span>1.2</span></p></td><td class="c64"><p class="c4 c2"><a name="h.sxfooeqscf76"></a><span>1.2</span></p></td></tr><tr class="c8"><td class="c61"><p class="c4 c2"><a name="h.o986boq4pg56"></a><span>Buffer</span></p></td><td class="c20"><p class="c4 c2"><a name="h.bnpzz3i5811g"></a><span>2</span></p></td><td class="c64"><p class="c4 c2"><a name="h.mgripwfa4eml"></a><span>2</span></p></td></tr><tr class="c8"><td class="c61"><p class="c4 c2"><a name="h.a5dgfsjas4of"></a><span>Ligase</span></p></td><td class="c20"><p class="c4 c2"><a name="h.lhmoobl0yz5y"></a><span>1</span></p></td><td class="c64"><p class="c4 c2"><a name="h.p261b7hwttvu"></a><span>1</span></p></td></tr><tr class="c8"><td class="c61"><p class="c4 c2"><a name="h.ez3nqb2i6p2c"></a><span>Water</span></p></td><td class="c20"><p class="c4 c2"><a name="h.om2zvwusi0oj"></a><span>6.6</span></p></td><td class="c64"><p class="c4 c2"><a name="h.bfarl4enbmbv"></a><span>15.8</span></p></td></tr><tr class="c8"><td class="c61"><p class="c4 c2"><a name="h.r8lccqmt9kep"></a><span>Total</span></p></td><td class="c20"><p class="c4 c2"><a name="h.ub6tthpcn1n9"></a><span>20</span></p></td><td class="c64"><p class="c4 c2"><a name="h.ksg5qvl5c9s7"></a><span>20</span></p></td></tr><tr class="c8"><td class="c61"><p class="c1 c4"><a name="h.z1but4l66e9c"></a></p></td><td class="c20"><p class="c4 c2"><a name="h.c2v6kg515cm9"></a><span>3</span></p></td><td class="c64"><p class="c4 c2"><a name="h.67vm5tw4jb6l"></a><span>4</span></p></td></tr></tbody></table><p class="c1"><a name="h.m76gb2uljerz"></a></p><p class="c2"><a name="h.308r4he0txvj"></a><span>1→ i5-39-1 (3:1 CP-Cra-pFri-RBS-GFP) → 100+ colonies</span></p><p class="c2"><a name="h.ghls9styo5zv"></a><span>2→ i5-39-2 (0:1)--> 300+ colonies</span></p><p class="c2"><a name="h.3ahcudohx7yw"></a><span>3→ i5-39-3 (3:! CP-Scr-pScr-RBS-GFP) → 200+ colonies</span></p><p class="c2"><a name="h.6vruk3cepto2"></a><span>4→ i5-39-4 (0:1) → 200+ colonies </span></p><p class="c2"><a name="h.ue2ilzk7m62u"></a><span>i5-11-5 → positive control (pLac-GFP) → TNTC</span></p><p class="c1"><a name="h.7nbtwclc1mi1"></a></p><p class="c2"><a name="h.a33r7clke9l9"></a><span>8/16</span></p><p class="c2"><a name="h.n3hd43aw1rcu"></a><span>Made glycerol stock</span></p><p class="c2"><a name="h.3539oxtcjglw"></a><span>i5-37-1 (CP weak -GFP)</span></p><p class="c2"><a name="h.6kzdjah0vru8"></a><span>i5-37-3 (CP medium-GFP)</span></p><p class="c2"><a name="h.nsiyrfar8tjx"></a><span>i5-37-5 (CP strong-GFP)</span></p>