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Team:Braunschweig/Protocols
From 2013.igem.org
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<h2><a href="#Spectra">Measurement of Absorption and Emission Spectra of the Chromoproteins</a></h2> | <h2><a href="#Spectra">Measurement of Absorption and Emission Spectra of the Chromoproteins</a></h2> | ||
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| + | <b>Absorption Spectra</b><br> | ||
| + | In order to measure the absorption spectra of the different chromoproteins 100 mL 2xYT medium containing chloramphenicol was inoculated with E. coli XL1 including pSB1C3 with chromoprotein expression cassette and grown over night at 37°C and 250 rpm in non-baffled flask to limit oxygen transfer in order to enhance chromoprotein expression. The whole culture volume was centrifuged for 10 min at 6000 rpm and the supernatant discarded. Cells were resolved in 5 mL PBS and disrupted using ultrasonic technology (see protein purification). Subsequently the cells were centrifuged again at 6000 rpm the supernatant was transferred into a new tube and measured with the nanodrop in a range between 190-840 nm.</p> | ||
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Revision as of 21:57, 23 September 2013
Protocols
In this section you will find detailed protocols of experimental procedures.
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