Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/4.2W3110LiquidCulture

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Phage Purification March - April Notebook: Experiments

4.2 W3110 Liquid Culture

I) Purpose

Grow bacteria for use in experiments.

II) Expected Outcome

Viable liquid culture of E. coli, W3110

III) Reagants Used

W3110 plated colonies

LB

IV) Actual Procedure

We put a colony from our W3110 plate of E.coli into 1mL LB broth and left in shaker at 37^◦ overnight.

Repeated 4 times, leaving a total of 5 1 ml colonies.

V) Results

Growth of W3110 liquid cultures of bacteria to create glycerol stocks from.