Template:Kyoto/Notebook/Aug 10

From 2013.igem.org

(Difference between revisions)
(→LB Medium Plate)
(→LB Medium Plate)
Line 48: Line 48:
|NaCl||1g
|NaCl||1g
|-
|-
-
|0.4µ NaOHaq||50µL
+
|0.4M NaOHaq||500µL
|-
|-
-
|Agar Pouder||2g　
+
|Agarose Pouder||2g　
 +
|-
 +
|Ampicillin||40µL
|}
|}
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Revision as of 06:46, 24 September 2013

Contents

Aug 10

Liquid Culture

Nakamoto

Samplemedium
8/9 BBa-J23106LB(+amp)
8/9 BBa-J23100-1LB(+amp)
8/9 BBa-J23100-2LB(+amp)
8/9 BBa-J44000-1LB(+amp)
8/9 BBa-J44000-2LB(+amp)

Waking Up Parts

Nakamoto

Sample
BBa-Q04400(RBS-tetR-DT-Ptet)pSB2K3
BBa-B0034(RBS)pSB1A2
BBa-I732020(RBS-lacZα-DTt)pSB1C3
BBa-P0412(RBS-lacI-DT)pSB1C3
BBa-R0011(Plac)pSB1C3

LB Medium Plate

Nakamoto & Tatsui

volume200ml
Bacto T2ypton 2g
Bacto yeast extract 1g
NaCl1g
0.4M NaOHaq500µL
Agarose Pouder2g　
Ampicillin40µL

Transformation

Gel Making

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