Template:Kyoto/Notebook/Aug 11

From 2013.igem.org

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(→Colony PCR)
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Revision as of 06:49, 24 September 2013

Aug 11

Miniprep

Nakamoto

DNAconcentration[µg/mL]260/280260/230
8/9 BBa-J44000-(2)100
8/9 BBa-J23106154
8/9 BBa-J23100144
8/10 T7-His-FT92

Colony PCR

Kojima and Yasuda

Samplebase pair
BBa-B0034-(2)-
BBa-B0034-(1)-
BBa-R0011-(2)-
BBa-R0011-(1)-
negative control-
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s20s30
Samplebase pair
BBa-D0412-(1)
BBa-D0412-(2)
negative control
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s1min30s30
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