Template:Kyoto/Notebook/Aug 11

From 2013.igem.org

(Difference between revisions)
(Colony PCR)
(Miniprep)
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<span class="author">Nakamoto</span>
<span class="author">Nakamoto</span>
{|class="wikitable"  
{|class="wikitable"  
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!DNA||concentration[&micro;g/mL]||260/280||260/230
+
!DNA||concentration[&micro;g/mL]
|-
|-
-
|8/9 BBa-J44000-(2)||100|| ||
+
|8/9 BBa_J44000 -(2)||100
|-
|-
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|8/9 BBa-J23106||154|| ||
+
|8/9 BBa_J23106||154
|-
|-
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|8/9 BBa-J23100||144|| ||
+
|8/9 BBa_J23100||144
|-
|-
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|8/10 T7-His-FT||92|| ||
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|8/10 T7-His-FT||92
|}
|}
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Revision as of 14:02, 24 September 2013

Aug 11

Miniprep

Nakamoto

DNAconcentration[µg/mL]
8/9 BBa_J44000 -(2)100
8/9 BBa_J23106154
8/9 BBa_J23100144
8/10 T7-His-FT92

Colony PCR

Kojima and Yasuda

Samplebase pair
BBa-B0034-(2)-
BBa-B0034-(1)-
BBa-R0011-(2)-
BBa-R0011-(1)-
negative control-
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s20s30
Samplebase pair
BBa-D0412-(1)
BBa-D0412-(2)
negative control
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s1min30s30