Template:Kyoto/Notebook/Aug 11
From 2013.igem.org
(Difference between revisions)
(→Colony PCR) |
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<span class="author">Nakamoto</span> | <span class="author">Nakamoto</span> | ||
{|class="wikitable" | {|class="wikitable" | ||
- | !DNA||concentration[µg/mL] | + | !DNA||concentration[µg/mL] |
|- | |- | ||
- | |8/9 | + | |8/9 BBa_J44000 -(2)||100 |
|- | |- | ||
- | |8/9 | + | |8/9 BBa_J23106||154 |
|- | |- | ||
- | |8/9 | + | |8/9 BBa_J23100||144 |
|- | |- | ||
- | |8/10 T7-His-FT||92 | + | |8/10 T7-His-FT||92 |
|} | |} | ||
</div> | </div> |
Revision as of 14:02, 24 September 2013
Aug 11
Miniprep
DNA | concentration[µg/mL] |
---|---|
8/9 BBa_J44000 -(2) | 100 |
8/9 BBa_J23106 | 154 |
8/9 BBa_J23100 | 144 |
8/10 T7-His-FT | 92 |
Colony PCR
Sample | base pair |
---|---|
BBa-B0034-(2) | - |
BBa-B0034-(1) | - |
BBa-R0011-(2) | - |
BBa-R0011-(1) | - |
negative control | - |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
94°C | 94°C | 55°C | 68°C | -- |
5min | 30s | 30s | 20s | 30 |
Sample | base pair |
---|---|
BBa-D0412-(1) | |
BBa-D0412-(2) | |
negative control |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
94°C | 94°C | 55°C | 68°C | -- |
5min | 30s | 30s | 1min30s | 30 |