Template:Kyoto/Notebook/Aug 11

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===Colony PCR===
===Colony PCR===
-
<!-- ここから -->
 
<div class="experiment">
<div class="experiment">
<span class="author">Kojima and Yasuda</span>
<span class="author">Kojima and Yasuda</span>
{| class="wikitable"
{| class="wikitable"
-
!Sample||base pair
+
!Sample
|-
|-
-
|BBa-B0034-(2)||-
+
|BBa_B0034 -(2)
|-
|-
-
|BBa-B0034-(1)||-
+
|BBa_B0034 -(1)
|-
|-
-
|BBa-R0011-(2)||-
+
|BBa_R0011 -(2)
|-
|-
-
|BBa-R0011-(1)||-
+
|BBa_R0011 -(1)
|-
|-
-
|negative control||-
+
|negative control
|}
|}
{| class="wikitable"
{| class="wikitable"
!PreDenature||Denature||Annealing||Extension||cycle
!PreDenature||Denature||Annealing||Extension||cycle
|-
|-
-
|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
+
|94 &deg;C||94 &deg;C||55 &deg;C||68 &deg;C||--
|-
|-
-
|5min||30s||30s||20s||30
+
|5 min||30 s||30 s||20 s||30 cycles
|}
|}
-
</div>
 
-
 
{| class="wikitable"
{| class="wikitable"
-
!Sample||base pair
+
!Sample
|-
|-
-
|BBa-D0412-(1)||
+
|BBa_D0412 -(1)
|-
|-
-
|BBa-D0412-(2)||
+
|BBa_D0412 -(2)
|-
|-
-
|negative control||
+
|negative control
|}
|}
{| class="wikitable"
{| class="wikitable"
!PreDenature||Denature||Annealing||Extension||cycle
!PreDenature||Denature||Annealing||Extension||cycle
|-
|-
-
|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
+
|94 &deg;C||94 &deg;C||55 &deg;C||68 &deg;C||--
|-
|-
-
|5min||30s||30s||1min30s||30
+
|5 min||30 s||30 s||1 min 30 s||30 cycles
|}
|}
 +
</div>

Revision as of 14:06, 24 September 2013

Aug 11

Miniprep

Nakamoto

DNAconcentration[µg/mL]
8/9 BBa_J44000 -(2)100
8/9 BBa_J23106154
8/9 BBa_J23100144
8/10 T7-His-FT92

Colony PCR

Kojima and Yasuda

Sample
BBa_B0034 -(2)
BBa_B0034 -(1)
BBa_R0011 -(2)
BBa_R0011 -(1)
negative control
PreDenatureDenatureAnnealingExtensioncycle
94 °C94 °C55 °C68 °C--
5 min30 s30 s20 s30 cycles
Sample
BBa_D0412 -(1)
BBa_D0412 -(2)
negative control
PreDenatureDenatureAnnealingExtensioncycle
94 °C94 °C55 °C68 °C--
5 min30 s30 s1 min 30 s30 cycles
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