Template:Kyoto/Notebook/Aug 11

From 2013.igem.org

(Difference between revisions)
(Colony PCR)
(Restriction Enzyme Digestion)
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===Restriction Enzyme Digestion===
===Restriction Enzyme Digestion===
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<div class="experiment">
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<span class="author">Kojima anda Yasuda</span>
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{| class="wikitable"
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!8/9 BBa_J44000 2||EcoRI||PstI||10x buffer H||MilliQ||total
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|-
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|2||0.2||0.2||1||6.6||10
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|-
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|2||0.2||--||1||6.8||10
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|-
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|2||--||0.2||1||6.8||10
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|-
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|2||--||--||1||7.0||10
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|}
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{| class="wikitable"
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!8/9 BBa_J23106||EcoRI||PstI||10x buffer H||MilliQ||total
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|-
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|1.3||0.2||0.2||1||7.3||10
 +
|-
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|1.3||0.2||--||1||7.5||10
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|-
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|1.3||--||0.2||1||7.5||10
 +
|-
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|1.3||--||--||1||7.7||10
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|}
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{| class="wikitable"
 +
!8/9 BBa_J23100 2||EcoRI||PstI||10x buffer H||MilliQ||total
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|-
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|1.3||0.2||0.2||1||7.3||10
 +
|-
 +
|1.3||0.2||--||1||7.5||10
 +
|-
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|1.3||--||0.2||1||7.5||10
 +
|-
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|1.3||--||--||1||7.7||10
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|}
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*incubated at 37 &deg;C for 1 hour.
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</div>
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===Electrophoresis===
===Electrophoresis===

Revision as of 14:26, 24 September 2013

Contents

Aug 11

Miniprep

Nakamoto

DNAconcentration[µg/mL]
8/9 BBa_J44000 -(2)100
8/9 BBa_J23106154
8/9 BBa_J23100144
8/10 T7-His-FT92

Colony PCR

Kojima and Yasuda

Sample
BBa_B0034 -(2)
BBa_B0034 -(1)
BBa_R0011 -(2)
BBa_R0011 -(1)
negative control
PreDenatureDenatureAnnealingExtensioncycle
94 °C94 °C55 °C68 °C--
5 min30 s30 s20 s30 cycles
Sample
BBa_D0412 -(1)
BBa_D0412 -(2)
negative control
PreDenatureDenatureAnnealingExtensioncycle
94 °C94 °C55 °C68 °C--
5 min30 s30 s1 min 30 s30 cycles

Restriction Enzyme Digestion

Kojima anda Yasuda

8/9 BBa_J44000 2EcoRIPstI10x buffer HMilliQtotal
20.20.216.610
20.2--16.810
2--0.216.810
2----17.010
8/9 BBa_J23106EcoRIPstI10x buffer HMilliQtotal
1.30.20.217.310
1.30.2--17.510
1.3--0.217.510
1.3----17.710
8/9 BBa_J23100 2EcoRIPstI10x buffer HMilliQtotal
1.30.20.217.310
1.30.2--17.510
1.3--0.217.510
1.3----17.710
  • incubated at 37 °C for 1 hour.

Electrophoresis