Team:Groningen/Labwork/24 September 2013

From 2013.igem.org

(Difference between revisions)
Line 21: Line 21:
<div class="mainContent">
<div class="mainContent">
 +
 +
<h2>Mirjam</h2>
 +
Three colonies from each plate of our silk constructs are taken and grown in LB medium with Cm. Did a mini prep reaction on these samples. To check if the samples are correct a restriction digestion is done.
 +
<br> A colony PCR is done for all of the &Delta;CheY and &Delta;CheY&Delta;Des strains. The gel showed bands for both the &Delta;CheY and &Delta;CheY&Delta;Des <i>B. subtilis</i> strains.
 +
<br>The primers for the &Delta;CheC mutant arrived. These are used to perform a PCR on the genomic DNA of <i>B. subtilis</i> 168 strain.
 +
<h2>Inne</h2>
<h2>Inne</h2>
Loaded a 0.8% gel with samples 1 till 4 of colony PCR from delta des of the double knockout strain. Mixture that was loaded contained 5,5 uL sample + 4,5 uL 2X loading dye. 5 uL 1kb marker was used.
Loaded a 0.8% gel with samples 1 till 4 of colony PCR from delta des of the double knockout strain. Mixture that was loaded contained 5,5 uL sample + 4,5 uL 2X loading dye. 5 uL 1kb marker was used.
Gel ran for 45 min at 90 volts.
Gel ran for 45 min at 90 volts.
 +
</div>
</div>

Revision as of 16:33, 24 September 2013

Mirjam

Three colonies from each plate of our silk constructs are taken and grown in LB medium with Cm. Did a mini prep reaction on these samples. To check if the samples are correct a restriction digestion is done.
A colony PCR is done for all of the ΔCheY and ΔCheYΔDes strains. The gel showed bands for both the ΔCheY and ΔCheYΔDes B. subtilis strains.
The primers for the ΔCheC mutant arrived. These are used to perform a PCR on the genomic DNA of B. subtilis 168 strain.

Inne

Loaded a 0.8% gel with samples 1 till 4 of colony PCR from delta des of the double knockout strain. Mixture that was loaded contained 5,5 uL sample + 4,5 uL 2X loading dye. 5 uL 1kb marker was used. Gel ran for 45 min at 90 volts.