Template:Kyoto/Notebook/Aug 10
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===Liquid Culture=== | ===Liquid Culture=== |
Revision as of 04:07, 25 September 2013
Contents |
Aug 10
Liquid Culture
Sample | medium |
---|---|
8/9 BBa-J23106 | LB(+amp) |
8/9 BBa-J23100-1 | LB(+amp) |
8/9 BBa-J23100-2 | LB(+amp) |
8/9 BBa-J44000-1 | LB(+amp) |
8/9 BBa-J44000-2 | LB(+amp) |
Waking Up Parts
Sample |
---|
BBa-Q04400(RBS-tetR-DT-Ptet)pSB2K3 |
BBa-B0034(RBS)pSB1A2 |
BBa-I732020(RBS-lacZα-DTt)pSB1C3 |
BBa-P0412(RBS-lacI-DT)pSB1C3 |
BBa-R0011(Plac)pSB1C3 |
LB Medium Plate(+Ampicillin)
volume | 200ml |
---|---|
Bacto T2ypton | 2g |
Bacto yeast extract | 1g |
NaCl | 1g |
0.4M NaOHaq | 500µL |
Agarose Pouder | 2g |
Ampicillin | 40µL |
Transformation
Name | Sample | Competent Cells | Total | Plate |
---|---|---|---|---|
tetR | 3µL | 19µL | 22µL | Kan |
RBS | 3µL | 19µL | 22µL | Amp |
lacZα | 3µL | 19µL | 22µL | CP |
lacI | 3µL | 19µL | 22µL | CP |
Plac | 3µL | 19µL | 22µL | CP |