Template:Kyoto/Notebook/Aug 10

From 2013.igem.org

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(→Colony PCR)
(→Colony PCR)
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!Sample
!Sample
|-
|-
-
|BBa_J23106
+
|8/9 BBa_J44000 -(1)
|-
|-
-
|BBa_J23100
+
|8/9 BBa_J44000 -(2)
|-
|-
-
|BBa_J44000
+
|NC
|}
|}
{| class="wikitable"
{| class="wikitable"
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|-
|-
|5 min||30 sec||30 sec||30 sec||30 cycles
|5 min||30 sec||30 sec||30 sec||30 cycles
 +
|}
 +
{| class="wikitable"
 +
!Sample
 +
|-
 +
|8/9 BBa_J23106
 +
|-
 +
|8/9 BBa_J23100 -(1)
 +
|-
 +
|8/9 BBa_J23100 -(2)
 +
|-
 +
|NC
 +
|}
 +
{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
 +
|-
 +
|94 °C||94 °C||55 °C||68 °C||--
 +
|-
 +
|5 min||30 sec||30 sec||1 min||30 cycles
|}
|}
</div>
</div>

Revision as of 04:28, 25 September 2013

Contents

Aug 10

Colony PCR

Nakamoto

Sample
8/9 BBa_J44000 -(1)
8/9 BBa_J44000 -(2)
NC
PreDenatureDenatureAnnealingExtensioncycle
94 °C94 °C55 °C68 °C--
5 min30 sec30 sec30 sec30 cycles
Sample
8/9 BBa_J23106
8/9 BBa_J23100 -(1)
8/9 BBa_J23100 -(2)
NC
PreDenatureDenatureAnnealingExtensioncycle
94 °C94 °C55 °C68 °C--
5 min30 sec30 sec1 min30 cycles

Electrophoresis

Liquid Culture

Nakamoto

Samplemedium
8/9 BBa-J23106LB(+amp)
8/9 BBa-J23100-1LB(+amp)
8/9 BBa-J23100-2LB(+amp)
8/9 BBa-J44000-1LB(+amp)
8/9 BBa-J44000-2LB(+amp)

Waking Up Parts

Nakamoto

Sample
BBa-Q04400(RBS-tetR-DT-Ptet)pSB2K3
BBa-B0034(RBS)pSB1A2
BBa-I732020(RBS-lacZα-DTt)pSB1C3
BBa-P0412(RBS-lacI-DT)pSB1C3
BBa-R0011(Plac)pSB1C3

LB Medium Plate(+Ampicillin)

Nakamoto & Tatsui

volume200ml
Bacto T2ypton 2g
Bacto yeast extract 1g
NaCl1g
0.4M NaOHaq500µL
Agarose Pouder2g　
Ampicillin40µL

Transformation

Hirano

NameSampleCompetent CellsTotalPlate
tetR3µL19µL22µLKan
RBS3µL19µL22µLAmp
lacZα3µL19µL22µLCP
lacI3µL19µL22µLCP
Plac3µL19µL22µLCP

Gel Making

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