Team:USTC CHINA/Modeling/

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<div id="breadcrumb"><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a> &gt; <a href="https://2013.igem.org/Team:USTC_CHINA/Modeling">Modeling</a></div></div>
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<h1>Modeling on the suicide mechanism of the reporter system</h1>
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<p>To eliminate potential safety problem, we constructed a suicide system on engineered bacteria to ensure biosafety. As the only one loaded with kill switch, the engineered reporter bacteria is responsible for eliminating all siblings in T-vaccine.
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Killing is mediated by the exported toxic protein SdpC. Extracellular SdpC induces the synthesis of an immunity protein, SdpI, which protects toxin-producing cells from being killed. SdpI, a polytopic membrane protein, is encoded by a two-gene operon under sporulation control that contains the gene for an autorepressor, SdpR.</p>
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  <img src="https://static.igem.org/mediawiki/2013/c/ce/Modeling_1.png" width="580" height="580" />
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<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/ReporterSystem">read more about Reporter System</a>
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the best growing condition for Bacillus subtilis WB800N
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<h1>the best growing condition for Bacillus subtilis WB800N</h1>
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<div class="port-sidebar-border"><h>Modeling</h></div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/ReporterSystem">Reporter System</a></div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">B.Subtilis Culture</a></div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/MiceModeling">Mice Modeling</a></div>
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Roughly, we could consider the treatment of No. 15 medium (Temperature 35℃, Time 12h, Peptone 15, Yeast Extract 7.5, NaCl 15)as the maximal condition for Bacillus subtilis.
Roughly, we could consider the treatment of No. 15 medium (Temperature 35℃, Time 12h, Peptone 15, Yeast Extract 7.5, NaCl 15)as the maximal condition for Bacillus subtilis.
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<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">read more about B.Subtilis Culture</a>
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<h1>Modeling on the suicide mechanism of the reporter system</h1>
 
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To eliminate potential safety problem, we constructed a suicide system on engineered bacteria to ensure biosafety. As the only one loaded with kill switch, the engineered reporter bacteria is responsible for eliminating all siblings in T-vaccine.
 
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Killing is mediated by the exported toxic protein SdpC. Extracellular SdpC induces the synthesis of an immunity protein, SdpI, which protects toxin-producing cells from being killed. SdpI, a polytopic membrane protein, is encoded by a two-gene operon under sporulation control that contains the gene for an autorepressor, SdpR.
 
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<h1>Mice Modeling</h1>
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<p>Our mice experiment has primarily proven the validity of our project. However, just like most scientific immune experiments on animals, the aim of our mice experiment was verification instead of exploring the optimal conditions for the production of our vaccine. In fact, fewer optimization experiments have been done by pure scientific researches, as most scientists care about facts and theories only, whereas exploring the optimal conditions is often viewed as the task of pharmaceutical factories. Yet since igem itself frequently involves industrial fields, which make igem seems like more an engineering competition than a science competition sometimes. </p>
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<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/MiceModeling">read more about Mice Moddeling</a>
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Revision as of 02:31, 26 September 2013

Modeling on the suicide mechanism of the reporter system

To eliminate potential safety problem, we constructed a suicide system on engineered bacteria to ensure biosafety. As the only one loaded with kill switch, the engineered reporter bacteria is responsible for eliminating all siblings in T-vaccine. Killing is mediated by the exported toxic protein SdpC. Extracellular SdpC induces the synthesis of an immunity protein, SdpI, which protects toxin-producing cells from being killed. SdpI, a polytopic membrane protein, is encoded by a two-gene operon under sporulation control that contains the gene for an autorepressor, SdpR.

read more about Reporter System

the best growing condition for Bacillus subtilis WB800N

Modeling

To ensure the expression of our T-vaccine, we do some experiments to find out the best growing condition for Bacillus subtilis WB800N, we finally choose five independent factors: temperature, inoculation time, peptone, yeast extract and NaCl. The following table displays their levels:

Factor Low High
Temperature 25℃ 35℃
Time 12h 24h
Peptone 5 15
Yeast Extract 2.5 7.5
NaCl 5 15

图无法导出 Roughly, we could consider the treatment of No. 15 medium (Temperature 35℃, Time 12h, Peptone 15, Yeast Extract 7.5, NaCl 15)as the maximal condition for Bacillus subtilis. <a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">read more about B.Subtilis Culture</a>


Mice Modeling

Our mice experiment has primarily proven the validity of our project. However, just like most scientific immune experiments on animals, the aim of our mice experiment was verification instead of exploring the optimal conditions for the production of our vaccine. In fact, fewer optimization experiments have been done by pure scientific researches, as most scientists care about facts and theories only, whereas exploring the optimal conditions is often viewed as the task of pharmaceutical factories. Yet since igem itself frequently involves industrial fields, which make igem seems like more an engineering competition than a science competition sometimes.

<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/MiceModeling">read more about Mice Moddeling</a>

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