Template:Kyoto/Notebook/Sep 25

(Difference between revisions)

Revision as of 09:38, 26 September 2013

Nakamoto

Sample	base pair
9/23 Pcon-PT181 attenuator-aptamer12-1R-DT-(5~12)	859
1527
9/23 Pcon-PT181 attenuator-pSB1C3-(5~8)	601

kojima and nakamoto

Name	Sample	Competent Cells	Total	Plate
Plac+tet aptamer12+Pcon tetR-DT+Ptet+RBS-GFP-DT	1µL	10µL	11µL	-
Pcon-pT181attenuator-DT+Pcon-tetR-DT+Ptet+RBS-GFP-DT	1µL	10µL	11µL	-
Pcon-tet aptamer12-DT+Pcon-tetR-DT+Ptet+RBS-GFP-DT	1µL	10µL	11µL	-
Pcon-Spinach-DT+Pcon-tetR-DT+Ptet+RBS-GFP-DT	1µL	10µL	11µL	-
Pcon-pT181antisense+DT+Pcon-tetR-DT+Ptet+RBS-GFP-DT	1µL	10µL	11µL	-
Pcon-pT181attenuator+tet aptamer12-DT+Pcon-tetR-DT+Ptet+RBS-GFP-DT	1µL	10µL	11µL	-
Pcon tetR-DT+Ptet+RBS-GFP-DT	1µL	10µL	11µL	-
Pcon tetR-DT+Pcon-GFP-DT+RBS-GFP-DT	1µL	10µL	11µL	-
Pcon-tet aptamer12-DT+Pcon-pT181attenuator+RBS-GFP-DT	1µL	10µL	11µL	-
Pcon-pT181attenuator-DT+Pcon-pT181attenuator+RBS-GFP-DT	1µL	10µL	11µL	-
Pcon-tet aptamer12-DT+Ptet-pT181antisense+Spinach-DT+Pcon-RBS-GFP-DT+RBS-GFP-DT	1µL	10µL	11µL	-
Pcon-tet aptamer12-DT+Pcon-pT181antisense+Spinach-DT+Pcon-pT181attenuator+RBS-GFP-DT	1µL	10µL	11µL	-
Pcon-tet aptamer12-DT+Ptet-pT181antisense+Spinach-DT+Pcon-pT181attenuator+RBS-GFP-DT	1µL	10µL	11µL	-

Name	Sample	Competent Cells	Total	Plate
aptamer12-DT(EcoRI+Xbal)+Pcon-pT181attenuator(EcoRI+Spel)	2µL	20µL	22µL	-
Ptet(Spel+Pstl)+RBS-GFP-DT(Xbal+Pstl))	2µL	20µL	22µL	-

nakamoto

state	Vector		Inserter		Ligation High ver.2
experiment	9/25 aptamer12-	xx µL	name	xx µL	xx µL
NC	name	xx µL	name	xx µL	xx µL

|experiment||name||xx µL||name||xx µL||xx µL |- |NC||name||xx µL||name||xx µL||xx µL

@@ Line 1: / Line 1: @@
 ==Sep 25==
+===Colony PCR===
+<div class="experiment">
+<span class="author">Nakamoto</span>
+{| class="wikitable"
+!Sample||base pair
+|-
+|9/23 Pcon-PT181 attenuator-aptamer12-1R-DT-(5~12)||859
+|-
+|9/23 Pcon-PT181 attenuator-RBS-GFP-DT-(5~8)|1527
+|-
+|9/23 Pcon-PT181 attenuator-pSB1C3-(5~8)||601
+|}
 ===Transformation===
-<!-- こっから -->
 <div class="experiment">
 <span class="author">kojima and nakamoto</span>