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Team:Chiba/Project/store
From 2013.igem.org
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| - | <h2 id="store" style="background-color:#ff9933"><center> | + | <h2 id="store" style="background-color:#ff9933"><center>Storage</center></h2> |
| - | <h3>Introduction</h3> | + | <h3>1.Introduction</h3> |
<p> Fe(Ⅱ) in <i>E.coli</i> causes Fenton reaction in response to hydrogen peroxide and produce hydroxyl radical (OH･) which is harmful to <i>E.coli</i>. As a result, giving iron to <i>E.coli</i> excessively leads to the death of <i>E.coli</i>. | <p> Fe(Ⅱ) in <i>E.coli</i> causes Fenton reaction in response to hydrogen peroxide and produce hydroxyl radical (OH･) which is harmful to <i>E.coli</i>. As a result, giving iron to <i>E.coli</i> excessively leads to the death of <i>E.coli</i>. | ||
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<br> These two effects enable isolation of iron in ferritin and produce E.coli with strong iron tolerance.</p> | <br> These two effects enable isolation of iron in ferritin and produce E.coli with strong iron tolerance.</p> | ||
</br></br></br></br></br></br></br></br></br></br></br></br></p> | </br></br></br></br></br></br></br></br></br></br></br></br></p> | ||
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| + | <h3>2.1.Plasmid</h3> | ||
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| + | <p> | ||
| + | パーツの事書く | ||
| + | <a href="#">link</a> | ||
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| + | </p> | ||
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| + | <h3>Result & Discussion</h3> | ||
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| + | <p> | ||
| + | <a href="#">Assay</a><br> | ||
| + | 実験結果書いていく | ||
| + | </p> | ||
Revision as of 09:49, 26 September 2013
Storage
1.Introduction
Fe(Ⅱ) in E.coli causes Fenton reaction in response to hydrogen peroxide and produce hydroxyl radical (OH･) which is harmful to E.coli. As a result, giving iron to E.coli excessively leads to the death of E.coli.
Ferritin is an intracellular protein which has a property to store iron. Ferritin has Heavy chain and Light chain. Heavy chain affects the oxidation of iron and stimulate 2Fe(Ⅱ)＋O2→[Fe(III)-O-O-Fe(III)] reaction. Light chain takes in Fe(Ⅲ) in ferritin. Fe2O3(H2O) has paramagnetism.
These two effects enable isolation of iron in ferritin and produce E.coli with strong iron tolerance.
2.1.Plasmid
パーツの事書く link
Result & Discussion
Assay
実験結果書いていく
