Team:Tianjin/Notebook

From 2013.igem.org

(Difference between revisions)
(→June 20th, 2013)
(→June 7th, 2013)
Line 556: Line 556:
<div class="img1">
<div class="img1">
<div>
<div>
-
<a href="#" target="_blank" ><img src="images/banner_1.jpg" width="400px" height="266px";/></a>
+
<a href="https://static.igem.org/mediawiki/2013/7/7f/Tju-gel-1.1.1.jpg" target="_blank" ><img src="https://static.igem.org/mediawiki/2013/7/7f/Tju-gel-1.1.1.jpg" width="400px" height="266px";/></a>
<div id="Enlarge">
<div id="Enlarge">
-
<a href="#"  title="Enlarge" target="_blank"><img src="https://static.igem.org/mediawiki/2013/9/90/Enlarge.jpg" width="20" height="20" alt="" align="right"/></a>
+
<a href="https://static.igem.org/mediawiki/2013/7/7f/Tju-gel-1.1.1.jpg"  title="Enlarge" target="_blank"><img src="https://static.igem.org/mediawiki/2013/9/90/Enlarge.jpg" width="20" height="20" alt="" align="right"/></a>
-
<b>Figure 1.</b> </div>
+
<b>Figure 1.</b>AlkR-1 -600bp cut from AlkR-1-pEASY-BLUNT</div>
</div></div>
</div></div>

Revision as of 15:48, 26 September 2013

  • June,2013
    • Sun Mon Tue Wed Thu Fri Sat
                  1
      2 3 4 5 6 7 8
      9 10 11 12 13 14 15
      16 17 18 19 20 21 22
      23 24 25 26 27 28 29
      30            
  • July,2013
    • Sun Mon Tue Wed Thu Fri Sat
        1 2 3 4 5 6
      7 8 9 10 11 12 13
      14 15 16 17 18 19 20
      21 22 23 24 25 26 27
      28 29 30 31      
  • August,2013
    • Sun Mon Tue Wed Thu Fri Sat
              1 2 3
      4 5 6 7 8 9 10
      11 12 13 14 15 16 17
      18 19 20 21 22 23 24
      25 26 27 28 29 30 31
  • September,2013
    • Sun Mon Tue Wed Thu Fri Sat
      1 2 3 4 5 6 7
      8 9 10 11 12 13 14
      15 16 17 18 19 20 21
      22 23 24 25 26 27 28
      29 30          

Contents

June 7th, 2013


Synthesized J23100/J23103-AlkR-tt-PalkM (tt=B1006) directly through pEASY-BLUNT plasmids, respectively.

gene 1

Figure 1.AlkR-1 -600bp cut from AlkR-1-pEASY-BLUNT

June 20th, 2013


Synthesized J23100/J23103-AlkR-tt-PalkM-RFP/tetA and linked them with pSB1C3, respectively.


gene


gene


gene


gene




June 26th, 2013


Synthesized J23100/J23114-NPDC-AAR with BamHI, SacI and KpnI recognition sites inserted through overlap PCR and linked them with pSB3K3, respectively.

gene


July, 5th, 2013


Constructed J23100/J23114-NPDC-AAR-pSB1K3, respectively.

gene


July 9th, 2013


Translated E. coli BL21 with plasmids constructed.


July, 22nd, 2013


Acquired YbhGFSR through PCR and linked it with pSB3C5.

gene 8


August, 1st, 2013


Constructed J23100-RBS（B0030）-YbhGFSR-pSB1C3 （BBa_K081005）.

gene


August, 10th, 2013


Constructed J23100-RBS（B0030）-YbhGFSR-pSB3C5.

gene 10


August 13th, 2013


Constructed J23100/J23103-AlkR-tt-pSB1C3 and PalkM-RFP/tetA-tt-pSB1C3 (tt=B0015), respectively.

gene


gene


gene



August, 18th, 2013


Constructed PalkM-RFP/tetA-tt-J23100/J23103-ALKR-tt-pSB1C3, respectively.

gene


gene


August 23rd, 2013


Constructed PalkM-RFP/tetA-tt-J23100/J23103-ALKR-tt-pSB4C5, respectively.

gene


gene

Retrieved from "http://2013.igem.org/Team:Tianjin/Notebook"