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Team:Chiba/Project/store
From 2013.igem.org
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Revision as of 17:30, 26 September 2013
Storage
1.Introduction
Fe(Ⅱ) in E.coli causes Fenton reaction in response to hydrogen peroxide and produce hydroxyl radical (OH･) which is harmful to E.coli. As a result, giving iron to E.coli excessively leads to the death of E.coli.
Ferritin is an intracellular protein which has a property to store iron. Ferritin has Heavy chain and Light chain. Heavy chain affects the oxidation of iron and stimulate 2Fe(Ⅱ)＋O2→[Fe(III)-O-O-Fe(III)] reaction. Light chain takes in Fe(Ⅲ) in ferritin. Fe2O3(H2O) has paramagnetism.
These two effects enable isolation of iron in ferritin and produce E.coli with strong iron tolerance.
2.1.Plasmid
パーツの事書く
Part link
2.2.Expression check
SDS pageについて書く
3.Results & Discussion
鉄を貯めるということは・・・・・
3.1.鉄耐性評価
Assay実験方法別ページ
実験結果書いてく
3.2.鉄取り込み量評価
Assay実験方法別ページ
実験結果書いてく
3.3.磁性評価
Assay実験方法別ページ
4.Conclusion
それは・・
