Team:NJU China/Team

From 2013.igem.org

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                         <h2 id="April">April</h2>
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                         <h2>2013</h2>
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                         <a class="ss-circle ss-circle-2">Week2 (14th-18th, April)</a>
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<a>siRNA screening (Failed)</a>
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<a>Dai Yimei</a>
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<span>14th: We extracted plasmids and cultured the 293t cells.</span>
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<span>Hi, my name is Dai Yimei and I am a senior at Nanjing University. I am majoring in life science and plan to pursue graduate study in developmental biology. I believe that science can work wonders.</br>  
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<span>15th: We transfected plasmids into 293t cells.</span>
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Contact: yimei1112@163.com</span>
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<span>16th: We collected cells and preserved it in Trizol.</span>
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<span>17th: We extracted RNA, then did RT-PCR with the RNA.</span>
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<span>18th: We did qPCR with the cDNA we got on 17th April.</span>
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<span>21th: We reexamined the concentration of RNA, and redid qPCR.</span>
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Revision as of 21:16, 26 September 2013

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Team

NJU_China

Gong Fei To strive,to seek, to find and never to give up.
Contact:gongfei1024@126.com

Dai Yimei Hi, my name is Dai Yimei and I am a senior at Nanjing University. I am majoring in life science and plan to pursue graduate study in developmental biology. I believe that science can work wonders.
Contact: yimei1112@163.com

siRNA screening (Failed) 27th: We cultured the 293t cells in a 12-well plate. 28th: We transfected 293t cells. 29th: We collected cells and extracted RNA from it. 30th: We did RT-qPCR with the RNA we extracted on 29th April. 1st: We did qPCR with the cDNA we got on 30th April.

May

2013

siRNA screening (Failed), examination whether siRNA are capsulated into exosomes (Success) 8th: We extracted plasmids of 3 kinds of siRNA. 9th: We cultured 293t cells in eight D10 dishes and a 12-well plate. 10th: We extracted 2 kinds of over-expression plasmids and examined the concentration of it. Then we transfect these plasmids into 293t cells respectively. 11th: We collected cells and exosomes from 8 D10 dish, and cells from 12-well plate. 12th: We extracted RNA from cells and exosomes. 13th: We did RT-PCR and qPCR.

August

2013

July

2013

June

2013