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Team:Evry/Notebook
From 2013.igem.org
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== Labwork: == | == Labwork: == | ||
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| + | '''Friday, June 21st''' | ||
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| + | The three following E. coli strains have been prepared to be chemically competent: BL21, DH5𝝰 and TOP10. | ||
| + | To check the quality of our work as well as their competence, each strain has been plated on LB medium with ampicillin, kanamycin or chloramphenicol. We also transformed our bacteria with a random plasmid and plated them on LB medium with ampicillin only. We incubated our petri dishes for the week end at 30°c | ||
Revision as of 16:09, 24 June 2013
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You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
Labwork:
Friday, June 21st
The three following E. coli strains have been prepared to be chemically competent: BL21, DH5𝝰 and TOP10. To check the quality of our work as well as their competence, each strain has been plated on LB medium with ampicillin, kanamycin or chloramphenicol. We also transformed our bacteria with a random plasmid and plated them on LB medium with ampicillin only. We incubated our petri dishes for the week end at 30°c
Monday, June 24th
Competents cells made on friday 21st was plated and incubated the whole week-end at 30°C. Today we analyzed results and it seems that medium had been contaminated.
New competents cells will be made.
