Latest revision as of 07:03, 27 September 2013

Maestro E.coli

Notebook

K1084001-010-B0034-lacZ-B0015

Start
PCR & PCR purification
- Sun Aug 11, Mon Sep 2
K1084007-B0034-mRFP1-B0015
100b up, random-oripro-r, KOD plus Neo
Digestion
- Mon Aug 12, Tue Sep 3
Cut random promoter(PCR product) with EcoRI, SpeI(using 10xH buffer)
Cut K1084007-B0034-mRFP1-B0015 on pSB1C3 with EcoRI, SpeI(using 10xH buffer)
Antarctic phosphatase
- Mon Aug 12, Wed Aug 14, Tue Sep 3
pSB1C3
Ligation
- Mon Aug 12, Wed Aug 14, Tue Sep 3
Ligate random promoter with pSB1C3
Transformation
- Mon Aug 12, Wed Aug 14, Tue Sep 3
random promoter on pSB1C3
1µL DNA to DH5α
Colony PCR
- Thu Aug 15, Thu Sep 5
random promoter on pSB1C3
Culture
- Thu Aug 15
K1084007-B0034-mRFP1-B0015 on pSB1C3
Mini-prep
- Fri Aug 16
K1084007-B0034-mRFP1-B0015 on pSB1C3
PCR
- Fri Aug 16
K1084007:100b up, oripro-r, KOD plus Neo
pSB1C3:Suf-I-F, XE-R, KOD plus Neo
EtOH precipitation
- Sat Aug 17
K1084007, pSB1C3
Digestion
- Sat Aug 17
Cut K1084007 with EcoRI, SpeI(using 10xH buffer)
Cut pSB1C3 with EcoRI, SpeI(using 10xH buffer)
Antarctic phosphatase
- Sun Aug 18
K1084007
Ligation
- Sun Aug 18
Ligate K1084007 with pSB1C3
Transformation
- Sun Aug 18
K1084007 on pSB1C3
1µL DNA to JM109
Transformation
- Mon Aug 19
random promoter on pSB1C3
1µL DNA to JM109
Colony PCR
- Wed Aug 21, Fri Sep 6
random promoter on pSB1C3
Ligation
- Thu Aug 22
Ligate random promoter with pSB1C3
Transformation
- Thu Aug 22
random promoter on pSB1C3
Colony PCR
- Wed Aug 28
random promoter on pSB1C3
Sequencing
- Fri Aug 30, Sun Sep 1, Mon Sep 2, Fri Sep 6, Mon Sep 9
random promoter on pSB1C3
Digestion
- Fri Sep 6
Cut I732020 with XbaI, PstI(using 10xM buffer)
Cut R0040-B0034-mRFP1-B0015 on pSB1C3 with EcoRI, PstI(using 10xH buffer)
Antarctic phosphatase
- Sun Sep 15
pSB1C3
Ligation
- Sun Sep 15
Ligate random promoter with B0034-lacZα-B0015, pSB1C3
Transformation
- Sun Sep 15
random promoter-B0034-lacZα-B0015 on pSB1C3
5µL DNA to JM109
Colony PCR
- Tue Sep 17
random promoter-B0034-lacZα-B0015 on pSB1C3
Culture
- Wed Sep 18, Thu Sep 19
random promoter-B0034-lacZα-B0015 on pSB1C3

random promoter:K1084001~010
Sequencing
- Thu Sep 19
K1084001~010-B0034-lacZα-B0015
200b dn
β-galactosidase assay
- Fri Sep 20, Thu Sep 26
K1084001~010-B0034-lacZα-B0015 on pSB1C3
Complete!

@@ Line 1: / Line 1: @@
+{{Team:HokkaidoU_Japan/header_Maestro}}
+<html>
+  <div id="common-header-bottom-background">
+    <div class="wrapper">
+      <h1 id="common-header-title">Maestro E.coli</h1>
+      <h2 id="common-header-subtitle">Notebook</h2>
+      <img id="common-header-img" src="https://static.igem.org/mediawiki/2013/e/ea/HokkaidoU2013_Maestro_Header.png">
+    </div>
+  </div>
+  <div class="wrapper">
+    <div id="hokkaidou-contents">
+<!-- end header / begin contents -->
+<h2>K1084001-010-B0034-lacZ-B0015</h2>
+<div class="workflow-container">
+  <ul class="steps">
+    <li class="step start">
+      <div class="step-tile"><h2>Start</h2></div>
+    </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">PCR & PCR purification</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sun Aug 11, Mon Sep 2</time> </li> </ul> <div class="step-recipe"> K1084007-B0034-mRFP1-B0015<br> 100b up, random-oripro-r, KOD plus Neo </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Digestion</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Mon Aug 12, Tue Sep 3</time> </li> </ul> <div class="step-recipe"> Cut random promoter(PCR product) with EcoRI, SpeI(using 10xH buffer)<br> Cut K1084007-B0034-mRFP1-B0015 on pSB1C3 with EcoRI, SpeI(using 10xH buffer) </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Antarctic phosphatase</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Mon Aug 12, Wed Aug 14, Tue Sep 3</time> </li> </ul> <div class="step-recipe"> pSB1C3 </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Ligation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Mon Aug 12, Wed Aug 14, Tue Sep 3</time> </li> </ul> <div class="step-recipe"> Ligate random promoter with pSB1C3 </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Transformation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Mon Aug 12, Wed Aug 14, Tue Sep 3</time> </li> </ul> <div class="step-recipe"> random promoter on pSB1C3<br> 1&micro;L DNA to DH5&alpha; </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Colony PCR</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Aug 15, Thu Sep 5</time> </li> </ul> <div class="step-recipe"> random promoter on pSB1C3 </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Culture</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Aug 15</time> </li> </ul> <div class="step-recipe"> K1084007-B0034-mRFP1-B0015 on pSB1C3 </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Mini-prep</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Aug 16</time> </li> </ul> <div class="step-recipe"> K1084007-B0034-mRFP1-B0015 on pSB1C3 </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">PCR</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Aug 16</time> </li> </ul> <div class="step-recipe"> K1084007:100b up, oripro-r, KOD plus Neo<br> pSB1C3:Suf-I-F, XE-R, KOD plus Neo </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">EtOH precipitation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Aug 17</time> </li> </ul> <div class="step-recipe"> K1084007, pSB1C3 </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Digestion</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Aug 17</time> </li> </ul> <div class="step-recipe"> Cut K1084007 with EcoRI, SpeI(using 10xH buffer) <br> Cut pSB1C3 with EcoRI, SpeI(using 10xH buffer) </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Antarctic phosphatase</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sun Aug 18</time> </li> </ul> <div class="step-recipe"> K1084007 </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Ligation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sun Aug 18</time> </li> </ul> <div class="step-recipe"> Ligate K1084007 with pSB1C3 </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Transformation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sun Aug 18</time> </li> </ul> <div class="step-recipe"> K1084007 on pSB1C3<br> 1&micro;L DNA to JM109 </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Transformation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Mon Aug 19</time> </li> </ul> <div class="step-recipe"> random promoter on pSB1C3<br> 1&micro;L DNA to JM109 </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Colony PCR</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Wed Aug 21, Fri Sep 6</time> </li> </ul> <div class="step-recipe"> random promoter on pSB1C3 </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Ligation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Aug 22</time> </li> </ul> <div class="step-recipe"> Ligate random promoter with pSB1C3 </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Transformation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Aug 22</time> </li> </ul> <div class="step-recipe"> random promoter on pSB1C3 </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Colony PCR</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Wed Aug 28</time> </li> </ul> <div class="step-recipe"> random promoter on pSB1C3 </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Sequencing</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Aug 30, Sun Sep 1, Mon Sep 2, Fri Sep 6, Mon Sep 9</time> </li> </ul> <div class="step-recipe"> random promoter on pSB1C3 </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Digestion</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Sep 6</time> </li> </ul> <div class="step-recipe"> Cut I732020 with XbaI, PstI(using 10xM buffer)<br> Cut R0040-B0034-mRFP1-B0015 on pSB1C3 with EcoRI, PstI(using 10xH buffer) </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Antarctic phosphatase</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sun Sep 15</time> </li> </ul> <div class="step-recipe"> pSB1C3 </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Ligation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sun Sep 15</time> </li> </ul> <div class="step-recipe"> Ligate random promoter with B0034-lacZ&alpha;-B0015, pSB1C3 </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Transformation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sun Sep 15</time> </li> </ul> <div class="step-recipe"> random promoter-B0034-lacZ&alpha;-B0015 on pSB1C3<br> 5&micro;L DNA to JM109 </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Colony PCR</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Tue Sep 17</time> </li> </ul> <div class="step-recipe"> random promoter-B0034-lacZ&alpha;-B0015 on pSB1C3 </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Culture</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Wed Sep 18, Thu Sep 19</time> </li> </ul> <div class="step-recipe"> random promoter-B0034-lacZ&alpha;-B0015 on pSB1C3<br> <br> random promoter:K1084001~010 </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">Sequencing</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Sep 19</time> </li> </ul> <div class="step-recipe"> K1084001~010-B0034-lacZ&alpha;-B0015<br> 200b dn </div> </div> </div> </li>
+<li class="step"> <div class="step-tile"> <h2 class="step-title">&beta;-galactosidase assay</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Sep 20, Thu Sep 26</time> </li> </ul> <div class="step-recipe"> K1084001~010-B0034-lacZ&alpha;-B0015 on pSB1C3 </div> </div> </div> </li>
+    <li class="step complete">
+      <div class="step-tile"><h2>Complete!</h2></div>
+    </li>
+  </ul>
+</div>
+<!-- end contents / begin footer -->
+    </div>
+  </div>
+</html>
+{{Team:HokkaidoU_Japan/footer}}

Team:HokkaidoU Japan/Notebook/Promoter K1084001~010-B0034-lacZ-B0015

From 2013.igem.org

Latest revision as of 07:03, 27 September 2013

HOKKAIDO U

Maestro E.coli

Notebook

K1084001-010-B0034-lacZ-B0015

Start

PCR & PCR purification

Digestion

Antarctic phosphatase

Ligation

Transformation

Colony PCR

Culture

Mini-prep

PCR

EtOH precipitation

Digestion

Antarctic phosphatase

Ligation

Transformation

Transformation

Colony PCR

Ligation

Transformation

Colony PCR

Sequencing

Digestion

Antarctic phosphatase

Ligation

Transformation

Colony PCR

Culture

Sequencing

β-galactosidase assay

Complete!