Team:KIT-Kyoto/Notebook/ATF2

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ATF2

10th August

We performed PCR to amplify the ATF2 gene.

F:3' CGATCCCATGGGGATGGAAGATATAGAAGGATACGAACCACA

R:5' CGATCGGATCCAAAAGCGACGCAAATTCGCCGATGGTTTG

Buffer	50uL
dNTP	20uL
Primer mix	1uL
DNA sample	0.5uL
KOD-FX	2uL
H₂O	26.5uL
Total	100uL

PCR products were electrophoresed in 1% agarose gel.

We detected a DNA band at around 1600bp.

PCR reactions were carried out (total volume was 300uL).

PCR products were electrophoresed in 1% agarose gel.

The PCR product with the size (1.6 kb) was verified as ATF2.

The PCR products of ATF2 was purified by ethanol precipitation

12th August

The PCR product of ATF2 were digested with the XhoI.

ATF2	5uL
Buff	2uL
H₂O	12uL
Xho1	1uL

No DNA was detected.

We have repeated the PCR reactions to amplify ATF2 under the same conditions as those carried out on 10th, August.

14th August

１．

PCR reaction was carried out by using the following primer for pSB1C3.

F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT

R:5' CGATCACTAGTATTAAAGCGACGCAAATTCGCCGATGGTTTG

PCR products were electrophoresed in 1% agarose gel.

The PCR product with the size (1.6 kb) was verified as ATF2.

The PCR products of ATF2 was purified by ethanol precipitation.

２．

The ATF2 gene and pET15b were purified by ethanol precipitation.

ATF2 was digested with the XhoI for verification.

ATF2	5uL
Buff	2uL
H₂O	12uL
Xho1	1uL

PCR products were electrophoresed in 1% agarose gel.

The PCR products with the expected size were detected.

ATF2 treated ethanol precipitation and pET-15b was digested with the NcoI and BamHI.

pET-15b	87uL
Nco1	1uL
BamH1	1uL
Buff.	10uL
BSA	1uL
(total 100uL)

ATF2	24uL
Nco1	1uL
BamH1	1uL
Buff.	3uL
BSA	1uL
(total 30uL)

It was electrophoresed in 1% Blue gel.

ATF2 were extracted from the Blue gel.

17th August

pET-15b was digested with the Nco1 and BamH1.

pET-15b	87uL
Nco1	1uL
BamH1	1uL
Buff.	10uL
BSA	1uL
(total 100uL)

We treated the vector with 1uL BAP for 30 minutes.

It was electrophoresed in 1% Blue gel

ATF2 were extracted from the Blue gel

19th August

The ATF2 gene and pET-15b were purified.

We ligated pET-15b and ATF2.

ATF2	5uL
pET-15b	5uL
L.MIX	10uL
total 20uL

After the ligatation, we have transformed them into E.coli cells.

20th August

We got approximately 70 colonies and cultured them furthermore.

21th August

<ATF2＋pET15b>

We checked the insertion of DNA by colony cracking.

We could see correct bands of plasmid DNA only from five samples.

We cultured each transformant in LB liquid medium containing ampicillin.

PCR reaction was carried out using the following primers

1-1

F:3' TCGGCAATGCACAGTCATGGCCATAGTAGA

R:5' TTATTCAATTGGATGAGAAATCACCTTTATGAAATCATGCT

1-2

F:3' CATCTTGAATTCGATGACTTGATTATGAATAATCAACCA

R:5' CGATCACTAGTATTAAAGCGACGCAAATTCGCCGATGGTTTG

2-1

F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT

R:5' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

2-2

F:3' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

R:5' ATCATCTTCGTAATCCTTGATGTGAATAGTGC

２

PCR products were electrophoresed in 1% agarose gel.

The PCR products with the size (1.6 kb) were verified as ATF2.

The PCR products of ATF2 were purified by ethanol precipitation and digested with the following composition.

1-1

ATF2	25uL
Mfe1	2uL
Buff.	3uL
Total 30uL

1-2

ATF2	25uL
EcoR1	2uL
Buff.	3uL
Total 30uL

2-1

ATF2	25uL
Nsi1	2uL
Buff.	3uL
Total 30uL

2-2

ATF2	25uL
Pst1	2uL
Buff.	3uL
Total 30uL

9

It were electrophoresed in 1% Blue gel

ATF2 were extracted from the Blue gel

22th August

Ⅰ

１

ATF2 gene extracted from the Blue gel was treated ethanol precipitation.

２

Ligation of 2-1 and 2-2 was performed by using Ligation mix.

1-1 with 1-2 and 2-1 with 2-2

The PCR products of ATF2 gene was purified.

It was electrophoresed in 1% agarose gel.

No DNA was detected.

Ⅱ

１

PCR reaction was carried out using the primers.

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

1-1

ATF2	25uL
Mfe1	2uL
Buff.	3uL
Total 30uL

1-2

ATF2	25uL
EcoR1	2uL
Buff.	3uL
Total 30uL

2-1

ATF2	25uL
Nsi1	2uL
Buff.	3uL
Total 30uL

2-2

ATF2	25uL
Pst1	2uL
Buff.	3uL
Total 30uL

It was electrophoresed in 1% Blue gel.

Any DNA fragment was not detected.

Ⅲ

PCR reaction was carried out using the following primers.

1-1

F:3' TCGGCAATGCACAGTCATGGCCATAGTAGA

R:5' TTATTCAATTGGATGAGAAATCACCTTTATGAAATCATGCT

1-2

F:3' CATCTTGAATTCGATGACTTGATTATGAATAATCAACCA

R:5'

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

1-1

ATF2	25uL
Mfe1	2uL
Buff.	3uL
Total 30uL

1-2

ATF2	25uL
EcoR1	2uL
Buff.	3uL
Total 30uL

It were electrophoresed in 1% Blue gel.

ATF2 were extracted from the Blue gel.

ATF2 extracted from the Blue gel was purified.

Ⅳ

PCR reaction was carried out using the primer.

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

1-1

ATF2	25uL
Mfe1	2uL
Buff.	3uL
Total 30uL

1-2

ATF2	25uL
EcoR1	2uL
Buff.	3uL
Total 30uL

23th August

The plasmids (d, e, f, I, o) were digested with Nco1 and BamH1.

We applied them to 1% agarose gel electrophoresis and checked them.

Sample d, e, f, i had two bands and we cultured them.

The plasmids (d, e, f, I,) were digested with BsiWI and checked.

We detected the correct size band.

We minipreped and transformed them into BL21(DE3).

Ⅰ

Sample 1.

ATF2 gene (Ⅳ) extracted from the Blue gel was purified by ethanol precipitation.

Sample 2.

ATF2 gene (Ⅲ) extracted from the Blue gel was purified by ethanol precipitation.

Ligation of sample 1 and 2 was performed by Ligation mix.

PCR reaction was carried out using the primers どの？？？？.

PCR products were electrophoresed in 1% agarose gel, and several fragments were detected.

PCR reaction was carried out using the primers どの？？？？.

Ⅱ

PCR reaction was carried out using the primers どの？？？？.

The PCR products of ATF2 gene were purified, and digested with Mfe and EcoRⅠ.

After the digestion, the ATF2 gene was electrophoresed in 1% Blue gel.

The ATF2 genes were extracted from the Blue gel and purified.

Ligation of 2-1 and 2-2 どれや？？？was performed using Ligation mix.

PCR reaction was carried out using the primers どの？？？？.(N)

24th August

Ⅰ

PCR reaction was carried out using the following primers.

2-1

F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT

R:5' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

2-2

F:3' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

R:5' ATCATCTTCGTAATCCTTGATGTGAATAGTGC

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

2-1

ATF2	25uL
Nsi1	2uL
Buff.	3uL
Total 30uL

2-2

ATF2	25uL
Pst1	2uL
Buff.	3uL
Total 30uL

It was electrophoresed in 1% Blue gel.

No DNA was detected.

Ⅱ

１

PCR react ion was carried out using the primer.

The PCR products for ATF2 was treated ethanol precipitation.

It was digested with the following composition.

2-1

ATF2	25uL
Nsi1	2uL
Buff.	3uL
Total 30uL

2-2

ATF2	25uL
Pst1	2uL
Buff.	3uL
Total 30uL

It were electrophoresed in 1% Blue gel.

ATF2 were extracted from the Blue gel.

ATF2 extracted from the Blue gel was purified.

Ligation of 2-1 and 2-2 was performed using Ligation mix.

Ⅲ

1.

PCR reaction was carried out using the primer.

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

ATF2	25uL
EcoR1	2uL
Buff.	3uL
Total 30uL

It was electrophoresed in 1% agarose gel.

Multiple DNA fragments were detected.

Ⅳ

１

PCR reaction was carried out using the primers.

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

ATF2	25uL
EcoR1	2uL
Buff.	3uL
Total 30uL

It was electrophoresed in 1% agarose gel.

Multiple DNA fragments were detected for the DIAP2.

26th August

Cell free extract was prepared and applied to SDS-PAGE to detect ATF2 protein.

27th August

PCR products were electrophoresed in 1% agarose gel.

The PCR products were treated ethanol precipitation.

They were digested.

They were electrophoresed in 1% Blue gel.

ATF2 were extracted from the Blue gel.

ATF2 extracted from the Blue gel was treated ethanol precipitation.

Ligation of 2-1 and 2-2 was performed using Ligation mix.

We purified them.

28th August

The PCR products was treated ethanol precipitation.

PCR reaction was carried out by using the primer.

PCR products were electrophoresed in 1% agarose gel.

No DNA was detected.

We performed PCR, purified, and digested again.

We ligated and purified them.

29th August

１

PCR reaction was carried out using the primer.

PCR products were electrophoresed in 1% agarose gel.

No DNA band was detected.

２

PCR reaction was carried out using the following primers.

2-1

F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT

R:5' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

2-2

F:3' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

R:5' ATCATCTTCGTAATCCTTGATGTGAATAGTGC

The PCR products were treated ethanol precipitation.

It was digested with the following composition.

2-1

ATF2	25uL
Nsi1	2uL
Buff.	3uL
Total 30uL

2-2

ATF2	25uL
Pst1	2uL
Buff.	3uL
Total 30uL

It was electrophoresed in 1% Blue gel.

ATF2 were extracted from the Blue gel.

ATF2 extracted from the Blue gel was purified.

We ligated them and purified it.

We performed PCR to amplify it.

Cell free extract was prepared with FastBreak Cell Lysis Reagent and applied to SDS-PAGE.

30th August

Ⅰ

１

PCR reaction was carried out using the following primers

1-1

F:3' TCGGCAATGCACAGTCATGGCCATAGTAGA

R:5' TTATTCAATTGGATGAGAAATCACCTTTATGAAATCATGCT

1-2

F:3' CATCTTGAATTCGATGACTTGATTATGAATAATCAACCA

R:5'

The PCR products were purfied.

It was digested with the following composition.

2-1

ATF2	25uL
Nsi1	2uL
Buff.	3uL
Total 30uL

2-2

ATF2	25uL
Pst1	2uL
Buff.	3uL
Total 30uL

It were electrophoresed in 1% Blue gel.

ATF2 were extracted from the Blue gel.

ATF2 extracted from the Blue gel was purified.

We ligated them and purified it.

PCR reaction was carried out using the following primers for pSB1C3.

PCR products were electrophoresed in 1% agarose gel.

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

ATF2	25uL
Pst1	2uL
Buff.	3uL
Total 30uL

PCR products were electrophoresed in 1% agarose gel.

The PCR products of ATF2 gene was purified.

Ⅱ

１

We performed PCR (prepared 8/29).

PCR reaction was carried out using the following primers.

2-1

F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT

R:5' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

2-2

F:3' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

R:5' ATCATCTTCGTAATCCTTGATGTGAATAGTGC

The PCR products were purified.

It was digested with the following composition.

2-1

ATF2	25uL
Nsi1	2uL
Buff.	3uL
Total 30uL

2-2

ATF2	25uL
Pst1	2uL
Buff.	3uL
Total 30uL

It was electrophoresed in 1% Blue gel.

ATF2 was extracted from the Blue gel.

ATF2 extracted from the Blue gel was purified.

We ligated it.

We purified it and performed PCR.

2^nd September

We purified the mutant of ATF2.

We digested it and cc3 with Xba1 and Spe1.

We electrophoresed and extracted them in and from 1% Blue gel.

We purified them.

We ligated and transformed them into E.coli cells.

4^th September

We purified the mutant of ATF2.

Three tubes of pSB1C3 were treated ethanol precipitation

We add 25mL H₂O and digested it with Xba1 and Spe1.

We electrophoresed and extracted them in and from 1% Blue gel.

We purified them.

We ligated and transformed them into E.coli cells.

5^th September

We did rapid check of the insert by colony cracking (ATF2+pSB1C3).

But we detected no band.

We cultured them for 5 hours and minipreped.

We checked them by electrophoresing but couldn’t see any bands.

So, we thought them failed.

We cultured other sample (ATF2+pSB1C3).

@@ Line 5: / Line 5: @@
 <head>
 </head>
-</html>
 <div class="document">
    <div id="doc-left">
-{{Template:KIT-Kyoto/menu_note}}
+<ul class="nav metro-nav-list">
+   <li class="nav-header">LabNote</li>
+   <li class="">
+      <a href="#ATF1">ATF1</a>
+      <ul class="nav">
+          <li><a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/ATF1/may">May</a></li>
+          <li><a href="">june</a></li>
+          <li><a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/ATF1/july">july</a></li>
+          <li><a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/ATF1/august">August</a></li>
+          <li><a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/ATF1/september">September</a></li>
+      </ul>
+   </li>
+   <li class="">
+      <a href="#https://2013.igem.org/Team:KIT-Kyoto/Notebook/ATF2">ATF2</a>
+      <ul class="nav">
+      </ul>
+   </li>
+   <li class="">
+      <a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/YJL">YJL068C</a>
+      <ul class="nav">
+      </ul>
+   </li>
+   <li class="">
+      <a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/growcurves">Growth Curves</a>
+      <ul class="nav">
+      </ul>
+   </li>
+  </ul>
    </div>
 <div id="doc-right" class="metro">
@@ Line 19: / Line 53: @@
-<div class=WordSection1 style='layout-grid:18.0pt'>
 <p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>10th August</span></b></p>
@@ Line 43: / Line 77: @@
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt;height:9.7pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>50µL</span></p>
+   style='font-size:11.0pt'>50uL</span></p>
    </td>
   </tr>
@@ Line 56: / Line 90: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:9.7pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>20µL</span></p>
+   style='font-size:11.0pt'>20uL</span></p>
    </td>
   </tr>
@@ Line 69: / Line 103: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:9.9pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>1µL</span></p>
+   style='font-size:11.0pt'>1uL</span></p>
    </td>
   </tr>
@@ Line 82: / Line 116: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:9.7pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>0.5µL</span></p>
+   style='font-size:11.0pt'>0.5uL</span></p>
    </td>
   </tr>
@@ Line 95: / Line 129: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:9.7pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>2µL</span></p>
+   style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 102: / Line 136: @@
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:9.9pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>H2O</span></p>
+   style='font-size:11.0pt'>H<sub>2</sub>O</span></p>
    </td>
    <td width=66 valign=top style='width:49.8pt;border-top:none;border-left:none;
@@ Line 108: / Line 142: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:9.9pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>26.5µL</span></p>
+   style='font-size:11.0pt'>26.5uL</span></p>
    </td>
   </tr>
@@ Line 121: / Line 155: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:9.7pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>100µL</span></p>
+   style='font-size:11.0pt'>100uL</span></p>
    </td>
   </tr>
@@ Line 133: / Line 167: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reactions were
-carried out (total volume was 300μl).</span></p>
+carried out (total volume was 300uL).</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR products were
@@ Line 166: / Line 200: @@
    <td width=61 valign=top style='width:46.1pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt;height:17.1pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5µL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5uL</span></p>
    </td>
   </tr>
@@ Line 177: / Line 211: @@
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 183: / Line 217: @@
    <td width=64 valign=top style='width:47.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>H20</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>H<sub>2</sub>O</span></p>
    </td>
    <td width=61 valign=top style='width:46.1pt;border-top:none;border-left:none;
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>12µL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>12uL</span></p>
    </td>
   </tr>
@@ Line 201: / Line 235: @@
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1µL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
    </td>
   </tr>
@@ Line 229: / Line 263: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
-carried out by using the following primer for psb1c3.</span></p>
+carried out by using the following primer for pSB1C3.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT</span></p>
@@ Line 266: / Line 300: @@
    <td width=61 valign=top style='width:46.1pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt;height:17.1pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5µL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5uL</span></p>
    </td>
   </tr>
@@ Line 277: / Line 311: @@
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 283: / Line 317: @@
    <td width=64 valign=top style='width:47.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>H20</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>H<sub>2</sub>O</span></p>
    </td>
    <td width=61 valign=top style='width:46.1pt;border-top:none;border-left:none;
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>12µL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>12uL</span></p>
    </td>
   </tr>
@@ Line 301: / Line 335: @@
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1µL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
    </td>
   </tr>
@@ Line 315: / Line 349: @@
 style='font-size:11.0pt;color:black'>ATF2</span></span><span lang=EN-US
 style='font-size:11.0pt;color:black'> </span><span lang=EN-US style='font-size:
-.0pt'>treated ethanol precipitation and pet15b was digested with the <i>Nco</i>I
+.0pt'>treated ethanol precipitation and pET-15b was digested with the <i>Nco</i>I
 and <i>Bam</i>HI.</span></p>
@@ Line 327: / Line 361: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>87µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>87uL</span></p>
    </td>
   </tr>
@@ Line 333: / Line 367: @@
    <td width=91 valign=top style='width:67.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Nco1 </span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nco</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1 </span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
    </td>
   </tr>
@@ Line 344: / Line 379: @@
    <td width=91 valign=top style='width:67.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BamH1 </span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bam</span></i><span
+  lang=EN-US style='font-size:11.0pt'>H1 </span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
    </td>
   </tr>
@@ Line 360: / Line 396: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10uL</span></p>
    </td>
   </tr>
@@ Line 371: / Line 407: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
    </td>
   </tr>
@@ Line 377: / Line 413: @@
    <td width=147 colspan=2 valign=top style='width:110.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:19.55pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>(total 100µl)</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>(total 100uL)</span></p>
    </td>
   </tr>
@@ Line 394: / Line 430: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24uL</span></p>
    </td>
   </tr>
@@ Line 400: / Line 436: @@
    <td width=98 valign=top style='width:73.45pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Nco1 </span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nco</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1 </span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
    </td>
   </tr>
@@ Line 411: / Line 448: @@
    <td width=98 valign=top style='width:73.45pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BamH1 </span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bam</span></i><span
+  lang=EN-US style='font-size:11.0pt'>H1 </span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
    </td>
   </tr>
@@ Line 427: / Line 465: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 438: / Line 476: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
    </td>
   </tr>
@@ Line 444: / Line 482: @@
    <td width=154 colspan=2 valign=top style='width:115.7pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:19.55pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>(total 30µl)</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>(total 30uL)</span></p>
    </td>
   </tr>
@@ Line 461: / Line 499: @@
 <p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>17th August</span></b></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PET15b was
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>pET-15b was
 digested with the <i>Nco</i>1 and <i>Bam</i>H1.</span></p>
@@ Line 473: / Line 511: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>87µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>87uL</span></p>
    </td>
   </tr>
@@ Line 479: / Line 517: @@
    <td width=91 valign=top style='width:67.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Nco1 </span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nco</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1 </span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
    </td>
   </tr>
@@ Line 490: / Line 529: @@
    <td width=91 valign=top style='width:67.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>BamH1 </span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Bam</span></i><span
+  lang=EN-US style='font-size:11.0pt'>H1 </span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
    </td>
   </tr>
@@ Line 506: / Line 546: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10uL</span></p>
    </td>
   </tr>
@@ Line 517: / Line 557: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
    </td>
   </tr>
@@ Line 523: / Line 563: @@
    <td width=147 colspan=2 valign=top style='width:110.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:19.55pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>(total 100µl)</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>(total 100uL)</span></p>
    </td>
   </tr>
 </table>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We treated the DNA
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We treated the
-with 1ul BAP for 30 minutes.</span></p>
+vector with 1uL BAP for 30 minutes.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>It was
@@ Line 548: / Line 588: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We ligated pET-15b
-with <i>ATF2</i>.</span></p>
+and <i>ATF2</i>.</span></p>
 <table class=MsoTableGrid border=1 cellspacing=0 cellpadding=0
@@ Line 561: / Line 601: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5uL</span></p>
    </td>
   </tr>
@@ Line 572: / Line 612: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5uL</span></p>
    </td>
   </tr>
@@ Line 583: / Line 623: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10uL</span></p>
    </td>
   </tr>
@@ Line 589: / Line 629: @@
    <td width=147 colspan=2 valign=top style='width:110.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>total 20µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>total 20uL</span></p>
    </td>
   </tr>
@@ Line 675: / Line 715: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products of
 <span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2</span></span>
-were purified by ethanol precipitation and digested with the following composition.</span></p>
+were purified by ethanol precipitation and digested with the following
+composition.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1-1</span></p>
@@ Line 690: / Line 731: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 696: / Line 737: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Mfe1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Mfe</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 712: / Line 754: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 718: / Line 760: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 738: / Line 780: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 744: / Line 786: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>EcoR1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Eco</span></i><span
+  lang=EN-US style='font-size:11.0pt'>R1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 760: / Line 803: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 766: / Line 809: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 787: / Line 830: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 793: / Line 836: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Nsi1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nsi</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 809: / Line 853: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 815: / Line 859: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 835: / Line 879: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 841: / Line 885: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Pst1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Pst</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 857: / Line 902: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 863: / Line 908: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 944: / Line 989: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 950: / Line 995: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Mfe1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Mfe</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 966: / Line 1,012: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 972: / Line 1,018: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 992: / Line 1,038: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 998: / Line 1,044: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>EcoR1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Eco</span></i><span
+  lang=EN-US style='font-size:11.0pt'>R1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 1,014: / Line 1,061: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 1,020: / Line 1,067: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 1,041: / Line 1,088: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 1,047: / Line 1,094: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Nsi1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nsi</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 1,063: / Line 1,111: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 1,069: / Line 1,117: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 1,089: / Line 1,137: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 1,095: / Line 1,143: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Pst1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Pst</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 1,111: / Line 1,160: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 1,117: / Line 1,166: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 1,170: / Line 1,219: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 1,176: / Line 1,225: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Mfe1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Mfe</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 1,192: / Line 1,242: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 1,198: / Line 1,248: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 1,218: / Line 1,268: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 1,224: / Line 1,274: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>EcoR1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Eco</span></i><span
+  lang=EN-US style='font-size:11.0pt'>R1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 1,240: / Line 1,291: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 1,246: / Line 1,297: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 1,290: / Line 1,341: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 1,296: / Line 1,347: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Mfe1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Mfe</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 1,312: / Line 1,364: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 1,318: / Line 1,370: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 1,338: / Line 1,390: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 1,344: / Line 1,396: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>EcoR1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Eco</span></i><span
+  lang=EN-US style='font-size:11.0pt'>R1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 1,360: / Line 1,413: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 1,366: / Line 1,419: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 1,378: / Line 1,431: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The plasmids (d,
-e, f, I, o) were digested with Nco1 and BamH1.</span></p>
+e, f, I, o) were digested with <i>Nco</i>1 and <i>Bam</i>H1.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We applied them to
@@ Line 1,440: / Line 1,493: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
-of <i>ATF2</i> gene were purified, and digested with Mfe and EcoR</span><span
+of <i>ATF2</i> gene were purified, and digested with <i>Mfe</i> and <i>Eco</i>R</span><span
 style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅰ</span><span lang=EN-US
 style='font-size:11.0pt'>.</span></p>
@@ Line 1,507: / Line 1,560: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 1,513: / Line 1,566: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Nsi1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nsi</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 1,529: / Line 1,583: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 1,535: / Line 1,589: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 1,555: / Line 1,609: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 1,561: / Line 1,615: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Pst1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Pst</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 1,577: / Line 1,632: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 1,583: / Line 1,638: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 1,623: / Line 1,678: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 1,629: / Line 1,684: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Nsi1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nsi</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 1,645: / Line 1,701: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 1,651: / Line 1,707: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 1,671: / Line 1,727: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 1,677: / Line 1,733: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Pst1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Pst</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 1,693: / Line 1,750: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 1,699: / Line 1,756: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 1,746: / Line 1,803: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 1,752: / Line 1,809: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>EcoR1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Eco</span></i><span
+  lang=EN-US style='font-size:11.0pt'>R1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 1,768: / Line 1,826: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 1,774: / Line 1,832: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 1,812: / Line 1,870: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 1,818: / Line 1,876: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>EcoR1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Eco</span></i><span
+  lang=EN-US style='font-size:11.0pt'>R1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 1,834: / Line 1,893: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 1,840: / Line 1,899: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 1,852: / Line 1,911: @@
 <p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>26th August</span></b></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Cell free extract
+was prepared and applied to SDS-PAGE to detect <i>ATF2</i> protein.</span></p>
 <p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
@@ Line 1,969: / Line 2,033: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 1,975: / Line 2,039: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Nsi1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nsi</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 1,991: / Line 2,056: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 1,997: / Line 2,062: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 2,017: / Line 2,082: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 2,023: / Line 2,088: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Pst1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Pst</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 2,039: / Line 2,105: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 2,045: / Line 2,111: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 2,066: / Line 2,132: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We performed PCR
 to amplify it.</span></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>&nbsp;</span></b></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>&nbsp;</span></b></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Cell free extract
+was prepared with FastBreak Cell Lysis Reagent and applied to SDS-PAGE.</span></p>
 <p class=MsoNormal><b><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></b></p>
@@ Line 2,093: / Line 2,166: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>R:5'</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products were
-were purfied.</span></p>
+purfied.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>It was digested
@@ Line 2,112: / Line 2,185: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 2,118: / Line 2,191: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Nsi1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nsi</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 2,134: / Line 2,208: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 2,140: / Line 2,214: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 2,160: / Line 2,234: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 2,166: / Line 2,240: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Pst1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Pst</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 2,182: / Line 2,257: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 2,188: / Line 2,263: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 2,208: / Line 2,283: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
-carried out using the following primers for Psb1c3.</span></p>
+carried out using the following primers for pSB1C3.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR products were
@@ Line 2,231: / Line 2,306: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 2,237: / Line 2,312: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Pst1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Pst</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 2,253: / Line 2,329: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 2,259: / Line 2,335: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 2,277: / Line 2,353: @@
 <p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>１</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We performed PCR (prepared
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We performed PCR
-/29).</span></p>
+(prepared 8/29).</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
@@ Line 2,320: / Line 2,396: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 2,326: / Line 2,402: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Nsi1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Nsi</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 2,342: / Line 2,419: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 2,348: / Line 2,425: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 2,368: / Line 2,445: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
    </td>
   </tr>
@@ Line 2,374: / Line 2,451: @@
    <td width=79 valign=top style='width:58.95pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Pst1</span></p>
+   <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>Pst</span></i><span
+  lang=EN-US style='font-size:11.0pt'>1</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border-top:none;border-left:
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
    </td>
   </tr>
@@ Line 2,390: / Line 2,468: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
    </td>
   </tr>
@@ Line 2,396: / Line 2,474: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30µl</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
    </td>
   </tr>
@@ Line 2,431: / Line 2,509: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We digested it and
-psb1c3 with <i>Xba</i>1 and <i>Spe</i>1.</span></p>
+cc3 with <i>Xba</i>1 and <i>Spe</i>1.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We electrophoresed
@@ Line 2,451: / Line 2,529: @@
 mutant of <i>ATF2</i>.</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Three tubes of
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Three tubes of pSB1C3
-psb1c3 were treated ethanol precipitation</span></p>
+were treated ethanol precipitation</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We add 25</span><span
 lang=EN-US style='font-size:11.0pt;font-family:Symbol'>m</span><span
-lang=EN-US style='font-size:11.0pt'>L H2O and digested it with <i>Xba</i>1 and <i>Spe</i>1.</span></p>
+lang=EN-US style='font-size:11.0pt'>L H<sub>2</sub>O and digested it with <i>Xba</i>1
+and <i>Spe</i>1.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We electrophoresed
@@ Line 2,474: / Line 2,553: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We did rapid check
-of the insert by colony cracking (<i>ATF2</i>+psb1c3).</span></p>
+of the insert by colony cracking (<i>ATF2</i>+pSB1C3).</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>But we detected no
@@ Line 2,489: / Line 2,568: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We cultured other
-sample (<i>ATF2</i>+psb1c3).</span></p>
+sample (<i>ATF2</i>+pSB1C3).</span></p>
 <p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
 </div>
 </div>
+</html>