Team:UTK-Knoxville/30 May 2013

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Revision as of 04:38, 26 June 2013

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Thursday 30 May 13

• Amplification of Aer, EnvZ, and OmpR from MG1655 gDNA:
  • Aer:
  • primers: Aer_f and Aer-EnvZ_1r
  • Tm: 52 and 55 (both worked the same, so lets use 55) Expected length: about 860bp
  • EnvZ
  • primers: Aer_f and Aer-EnvZ_1r
  • Tm: 57 and 60 (both worked the same, so lets use 60)
  • Expected length: about 650bp
  • OmpR
  • primers: OmpR_r and OmpR_f
  • Tm: 60
  • Expected length: 720bp
  • Julie ran the gel and they all looked good.
  • Overlapping PCR of Aer/EnvZ
  • primers: Aer_f and EnvZ_r
  • expected length: 1490bp
  • The gel was kind of streaky because Julie changed something on the machine Julie shouldn’t have, but the band we wanted was still there. The Team had a fiasco with the gel kit though. Julie ran it back through a gel to see if it was still there and it wasn’t, so we need to redo that.
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