Team:HokkaidoU Japan/Overview

From 2013.igem.org

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           Recently, more and more projects for iGEM involve precisely regulated systems or Multistep catalysis. These biological systems need to have
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           Recently, more and more projects for iGEM involve precisely regulated systems or multistep catalysis. These biological systems need to have
           <ol>
           <ol>
             <li>transparent structure</li>
             <li>transparent structure</li>
             <li>reliable function</li>
             <li>reliable function</li>
             <li>reproducibility</li>
             <li>reproducibility</li>
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             <li>safety</li>
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             <li>and be safe</li>
           </ol>
           </ol>
           Especially parts controlling gene expression such as promoters or RBSs, it is desired that their prospective functions are explainable.
           Especially parts controlling gene expression such as promoters or RBSs, it is desired that their prospective functions are explainable.
         </p>
         </p>
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         <p>This year, we will make well-selected sets of promoters and RBSs with dynamic range of strength. These parts are evaluated strictly. We mutate a region of a basic part to make these sets, so their performances are reliable.</p>
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         <p>This year, we made well-selected sets of promoters and RBSs with dynamic range of strength. We mutated a -35 region of a promoter to make a family of variable promoters. These parts were strictly evaluated. </p>
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         <p>Furthermore, we make the Optimization Kit which enables you to select the most suitable parts for your project by only one step. This Kit will help other iGEMers create more complex and effective devises.</p>
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         <p>Furthermore, we make the expresion Optimization Kit which enables you to select the most suitable promoters and RBSs for your project. This can be accomplished in only one step. This Kit will help other iGEMers create more complex and effective devises.</p>
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         <p>Besides, we gave a presentation at our university and some high schools about iGEM and genetics. On that occasion we get to know how the people think about gene recombination by means of questionnaires and try to provide them with information for deeper thought. We hope these activities will lead the society where people accept genetic engineering technology. </p>
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         <p>We also did presentation at our university and two high schools about iGEM and genetics. On that occasion we get to know what the people think about gene recombination by means of questionnaires. We tried to provide them with information for deeper thought. We hope these activities will lead the society where people are more accepting genetic engineering. </p>
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Revision as of 15:19, 27 September 2013

Maestro E.coli

Overview

Project Description

Recently, more and more projects for iGEM involve precisely regulated systems or multistep catalysis. These biological systems need to have

  1. transparent structure
  2. reliable function
  3. reproducibility
  4. and be safe
Especially parts controlling gene expression such as promoters or RBSs, it is desired that their prospective functions are explainable.

This year, we made well-selected sets of promoters and RBSs with dynamic range of strength. We mutated a -35 region of a promoter to make a family of variable promoters. These parts were strictly evaluated.

Furthermore, we make the expresion Optimization Kit which enables you to select the most suitable promoters and RBSs for your project. This can be accomplished in only one step. This Kit will help other iGEMers create more complex and effective devises.

We also did presentation at our university and two high schools about iGEM and genetics. On that occasion we get to know what the people think about gene recombination by means of questionnaires. We tried to provide them with information for deeper thought. We hope these activities will lead the society where people are more accepting genetic engineering.