"
Team:BYU Provo/Notebook/LargePhage/Summerexp/Period3/Dailylog
From 2013.igem.org
| Line 35: | Line 35: | ||
<font size="4"> '''8/2/13''' </font> | <font size="4"> '''8/2/13''' </font> | ||
| + | |||
Today we set up an experiment which we will do on 8/7/13. We re-picked the plaques from the plate of mutagenized phage. The plate was full of variations in plaque sizes. We took the biggest plaques and put them in a mL of LB broth in an eppendorf. | Today we set up an experiment which we will do on 8/7/13. We re-picked the plaques from the plate of mutagenized phage. The plate was full of variations in plaque sizes. We took the biggest plaques and put them in a mL of LB broth in an eppendorf. | ||
We also started to take a closer look at our EMs from Wednesday. We are slowly learning how to use the program imageJ to our best use. | We also started to take a closer look at our EMs from Wednesday. We are slowly learning how to use the program imageJ to our best use. | ||
| + | |||
[[File:August10.jpg|400px]] | [[File:August10.jpg|400px]] | ||
| + | |||
| + | <br> | ||
<font size="4"> '''8/5/13''' </font> | <font size="4"> '''8/5/13''' </font> | ||
| + | |||
Today we spent class working with ImageJ to measure the phage from the various categories in the cesium chloride gradient. | Today we spent class working with ImageJ to measure the phage from the various categories in the cesium chloride gradient. | ||
| + | |||
| + | <br> | ||
<font size="4"> '''8/7/13''' </font> | <font size="4"> '''8/7/13''' </font> | ||
| + | |||
Today we picked from small plaques and diluted them in 1 mL of LB. We didn't have grown bacteria so we just put the tubes in the fridge. | Today we picked from small plaques and diluted them in 1 mL of LB. We didn't have grown bacteria so we just put the tubes in the fridge. | ||
We also drafted our project description as a class and posted it to our web site. | We also drafted our project description as a class and posted it to our web site. | ||
| + | |||
| + | <br> | ||
<font size="4"> '''8/9/13''' </font> | <font size="4"> '''8/9/13''' </font> | ||
| + | |||
We also discussed the need to separate the phage from the CsCl gradient into fractions so we don't risk contaminating the large phage fraction with normal phage from the large, central band. | We also discussed the need to separate the phage from the CsCl gradient into fractions so we don't risk contaminating the large phage fraction with normal phage from the large, central band. | ||
Results from 8/7/13 plaque test: | Results from 8/7/13 plaque test: | ||
| + | |||
| + | |||
[[File:Aug7-1.jpg|450px]] | [[File:Aug7-1.jpg|450px]] | ||
| + | |||
[[File:Aug7-2.jpg|450px]] | [[File:Aug7-2.jpg|450px]] | ||
| + | |||
| + | <br> | ||
<font size="4"> '''8/12/13''' </font> | <font size="4"> '''8/12/13''' </font> | ||
| + | |||
Today we gave the phage purification group another round of mutagenized phage to run through a CsCl gradient. They will separate each band into fractions. | Today we gave the phage purification group another round of mutagenized phage to run through a CsCl gradient. They will separate each band into fractions. | ||
| + | |||
| + | <br> | ||
<font size="4"> '''8/14/13''' </font> | <font size="4"> '''8/14/13''' </font> | ||
| + | |||
| + | |||
[[File:Aug14-1.jpg|400px]] | [[File:Aug14-1.jpg|400px]] | ||
| + | |||
[[File:Aug14-2.jpg|400px]] | [[File:Aug14-2.jpg|400px]] | ||
| + | <br> | ||
{{TeamBYUProvoFooter}} | {{TeamBYUProvoFooter}} | ||
Revision as of 18:03, 27 September 2013
| ||
|
|
8/2/13 Today we set up an experiment which we will do on 8/7/13. We re-picked the plaques from the plate of mutagenized phage. The plate was full of variations in plaque sizes. We took the biggest plaques and put them in a mL of LB broth in an eppendorf. We also started to take a closer look at our EMs from Wednesday. We are slowly learning how to use the program imageJ to our best use.
8/5/13 Today we spent class working with ImageJ to measure the phage from the various categories in the cesium chloride gradient.
8/7/13 Today we picked from small plaques and diluted them in 1 mL of LB. We didn't have grown bacteria so we just put the tubes in the fridge. We also drafted our project description as a class and posted it to our web site.
8/9/13 We also discussed the need to separate the phage from the CsCl gradient into fractions so we don't risk contaminating the large phage fraction with normal phage from the large, central band. Results from 8/7/13 plaque test:
8/12/13 Today we gave the phage purification group another round of mutagenized phage to run through a CsCl gradient. They will separate each band into fractions.
8/14/13
|
|
