Team:Chiba/Project/Future

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Revision as of 19:11, 27 September 2013

iGEM-2013 Chiba

Future

Tasks we couldn’t finish until Asia contest
    ・We couldn’t double knock down fieF and fur with CRISPRi yet, but we are confident that we can knock down any gene by CRISPRi.
    ・In the not so distant future, we would break E. coli’s iron homeostasis using CRISPERi and would uptake more iron into E. coli.
    ・Even if E. coli’s iron homeostasis would break, over expression of human ferritin could save E. coli alive.

Tasks that didn’t go like we wanted
    ・knocking out gor or trxB didn’t lead to a grate change of redox potential like TCO89 overexpression in yeast.
    ・It might be hard to oxidize iron in E. coli strain SHuffle®(It isn’t oxidized enough). But by over expression of strong oxidase, maybe we can oxidize iron inside E. coli, make magnetite and magnetize E. coli.

What is in future?
    We decided to design & implement biobricks that are effective for magnetizing E. coli.
    If we can freely magnetize any given cells, together with the cell-surface display techniques, you can establish novel systems for bioseparations. Or, you can simplify the harvesting/ collecting step in the process of bioproduction and bioremediation.
    E. coli would proliferates infinitely if we feed them and give them iron. So if you use it to a magnetic recording media, perhaps the storage capacity could increase infinitely.

@@ Line 25: / Line 25: @@
 <br>&nbsp;&nbsp;&nbsp;&nbsp;・It might be hard to oxidize iron in <i>E. coli</i> strain <a href="http://www.nebj.jp/products/detail/113">SHuffle®</a>(It isn’t oxidized enough). But by over expression of strong oxidase, maybe we can oxidize iron inside <i>E. coli</i>, make magnetite and magnetize <i>E. coli</i>.
-<br><br><b>A Use of magnetized <i>E. coli</i></b>
+<br><br><b>What is in future?</b>
-<br>&nbsp;&nbsp;&nbsp;&nbsp;・<i>E. coli</i> would proliferates infinitely if we feed them and give them iron. So if we use it to a magnetic recording media, perhaps the storage capacity could increase infinitely.
+<br>&nbsp;&nbsp;&nbsp;&nbsp;We decided to design & implement biobricks that are effective for magnetizing E. coli.
-<br>&nbsp;&nbsp;&nbsp;&nbsp;・Magnetized <i>E. coli</i> could be attracted by magnetic force, so we can separate magnetized <i>E. coli</i> easily from the colony of <i>E. coli</i>.
+<br>&nbsp;&nbsp;&nbsp;&nbsp;If we can freely magnetize any given cells, together with the cell-surface display techniques, you can establish novel systems for bioseparations. Or, you can simplify the harvesting/ collecting step in the process of bioproduction and bioremediation.
-<br>&nbsp;&nbsp;&nbsp;&nbsp;・Combining with some BioBrick (i.e.University of California Berkley 2006, Addressable Conjugation in Bacterial Networks), we can make a magnetic switch. Also, in combination with quorum sensing, we can make a magnetic switch.
+<br>&nbsp;&nbsp;&nbsp;&nbsp;<i>E. coli</i> would proliferates infinitely if we feed them and give them iron. So if you use it to a magnetic recording media, perhaps the storage capacity could increase infinitely.
-Magnetic switch has good points like we wrote in introduction.