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Team:Buenos Aires/ res rfpdeg
From 2013.igem.org
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| - | = GFP under Arsenite Inducible Promoter | + | = GFP under Arsenite Inducible Promoter [http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004] = |
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| - | Several cultures carrying a plasmid that encodes GFP under this promoter(Bba_K1106004) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm). | + | Several cultures carrying a plasmid that encodes GFP under this promoter([http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004]) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm). |
Revision as of 19:52, 27 September 2013
GFP under Arsenite Inducible Promoter [http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004]
Objective
Characterize the response of the arsenite sensitive promoter measuring GFP production upon different arsenite concrentrations.
Methods
Several cultures carrying a plasmid that encodes GFP under this promoter([http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004]) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm).
Results
As shown in the graphics below GFP production increases with the arsenite concentration.
Conclusion
The promoter is sensitive to different arsenite concentrations.
