COLONPCR Protocol

From 2013.igem.org

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(Created page with "===COLONY PCR Protocol for PCR machines=== #98ºC for '''2 min''' #98ºC for '''15 sec''' #60ºC for '''15 sec''' #72ºC for '''2 min''' #repeat steps 2-4 '''31 times''' #72ºC f...")
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Revision as of 20:06, 27 September 2013

COLONY PCR Protocol for PCR machines

  1. 98ºC for 2 min
  2. 98ºC for 15 sec
  3. 60ºC for 15 sec
  4. 72ºC for 2 min
  5. repeat steps 2-4 31 times
  6. 72ºC for 4 min
  7. Hold at 4ºC

Estimated Time: 1 hr 45 min

Retrieved from "http://2013.igem.org/COLONPCR_Protocol"