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Template:Kyoto/Notebook/Aug 10
From 2013.igem.org
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| - | |8/9 BBa-J23106||LB(+ | + | |8/9 BBa-J23106||LB(+Amp) |
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|8/9 BBa-J23100-1||LB(+Amp) | |8/9 BBa-J23100-1||LB(+Amp) | ||
Revision as of 21:11, 27 September 2013
Contents |
Aug 10
Colony PCR
| Sample |
|---|
| 8/9 BBa_J44000 -(1) |
| 8/9 BBa_J44000 -(2) |
| NC |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 94 °C | 94 °C | 55 °C | 68 °C | -- |
| 5 min | 30 sec | 30 sec | 30 sec | 30 cycles |
| Sample |
|---|
| 8/9 BBa_J23106 |
| 8/9 BBa_J23100 -(1) |
| 8/9 BBa_J23100 -(2) |
| NC |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 94 °C | 94 °C | 55 °C | 68 °C | -- |
| 5 min | 30 sec | 30 sec | 1 min | 30 cycles |
Electrophoresis
| Lane | Sample |
|---|---|
| 100bp ladder | |
| 4 | 8/9 BBa_J44000 -1 |
| 5 | 8/9 BBa_J44000 -2 |
| 6 | NC |
| Lane | Sample |
|---|---|
| 1kbp ladder | |
| 1 | 8/9 BBa_J23106 |
| 2 | 8/9 BBa_J23100 -1 |
| 3 | 8/9 BBa_J23100 -2 |
| 4 | NC |
Liquid Culture
| Sample | medium |
|---|---|
| 8/9 BBa-J23106 | LB(+Amp) |
| 8/9 BBa-J23100-1 | LB(+Amp) |
| 8/9 BBa-J23100-2 | LB(+Amp) |
| 8/9 BBa-J44000-1 | LB(+Amp) |
| 8/9 BBa-J44000-2 | LB(+Amp) |
LB Medium Plate(+Ampicillin)
| volume | 200ml |
|---|---|
| Bacto T2ypton | 2g |
| Bacto yeast extract | 1g |
| NaCl | 1g |
| 0.4M NaOHaq | 500µL |
| Agarose Pouder | 2g |
| Ampicillin | 40µL |
Transformation
| Name | Sample | Competent Cells | Total | Plate |
|---|---|---|---|---|
| tetR | 3µL | 19µL | 22µL | Kan |
| RBS | 3µL | 19µL | 22µL | Amp |
| lacZα | 3µL | 19µL | 22µL | CP |
| lacI | 3µL | 19µL | 22µL | CP |
| Plac | 3µL | 19µL | 22µL | CP |
