Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/6.10PhagePreparation

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Latest revision as of 00:29, 28 September 2013


Phage Purification May - June Notebook: Experiments



Phage Purification
March-April
May-June
July-August
September-October

6.10 Phage Preparation


I) Purpose

Grow phage to practice purification procedures.

II) Expected Outcome

Viable stocks of both T4 and T7.

III) Reagants Used

W3110 and BL21 freezer stocks
LB
T4 and T7 phage stocks


IV) Actual Procedure

Infect 45 mL of LB with 2 loop-fulls of bacterial freezer stock and let sit at 37◦ C overnight.
Infect W3110 with 100 µL T4 and BL21 with 100 µL T7.
Let both cultures sit overnight at 37◦ C.

V) Results

We created phage cultures to practice the purification procedure.


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