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Team:LZU-China/Project
From 2013.igem.org
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<h3> <span class="mw-headline" id="Promoter">Promoter: 4 copies of NFκB gene </span></h3> | <h3> <span class="mw-headline" id="Promoter">Promoter: 4 copies of NFκB gene </span></h3> | ||
On the basis of NF-κB signaling pathway and its feedback loop dynamics, we provided a device: A high-throughput drug screening system. The system is used to screen targeted drugs in NF-κB signaling pathway and lasts even longer. Natrually, we could estimate whether the kind of drug is good or not. | On the basis of NF-κB signaling pathway and its feedback loop dynamics, we provided a device: A high-throughput drug screening system. The system is used to screen targeted drugs in NF-κB signaling pathway and lasts even longer. Natrually, we could estimate whether the kind of drug is good or not. | ||
| - | <br><img src="https://static.igem.org/mediawiki/2013/ | + | <br><img src="https://static.igem.org/mediawiki/2013/6/64/LZU-China_Project_1.png"/> |
<br><br>NF-κB is a DNA binding protein, which means that the dimer could recognize a certain gene sequence and bind with it. Thus, we designed a four coupies of NF-κB gene sequence as the promoter. The downstream gene IκB flag with GFP, which could be a controller in tumor invading，moreover ， easy to observe. | <br><br>NF-κB is a DNA binding protein, which means that the dimer could recognize a certain gene sequence and bind with it. Thus, we designed a four coupies of NF-κB gene sequence as the promoter. The downstream gene IκB flag with GFP, which could be a controller in tumor invading，moreover ， easy to observe. | ||
Revision as of 01:32, 28 September 2013
Design & Device
Promoter: 4 copies of NFκB gene
On the basis of NF-κB signaling pathway and its feedback loop dynamics, we provided a device: A high-throughput drug screening system. The system is used to screen targeted drugs in NF-κB signaling pathway and lasts even longer. Natrually, we could estimate whether the kind of drug is good or not.
NF-κB is a DNA binding protein, which means that the dimer could recognize a certain gene sequence and bind with it. Thus, we designed a four coupies of NF-κB gene sequence as the promoter. The downstream gene IκB flag with GFP, which could be a controller in tumor invading，moreover ， easy to observe.
Expriments
LanZhou University | 222 TianShui South Road, ChengGuan District, LanZhou, China, 730000
E-mail:hwang2013@lzu.edu.cn
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E-mail:hwang2013@lzu.edu.cn
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