Team:BYU Provo/Results

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Revision as of 01:52, 28 September 2013

Achievements

Phage Library

This summer, we developed a cesium chloride gradient protocol for isolating larger and smaller phage. Utilizing this gradient, we were able to isolate mutant T4 bacteriophages that have distinctively larger or smaller capsid. Similarly, we were also able to isolate mutant T7 bacteriophages that have smaller than average capsid size and less variability in capsid size when compared to the wild type.

Cholera

To tackle the disease Cholera, we identified a protein, Amylase, that disrupt Cholera's biofilm formation. On our way to achieving our goals of engineering E. coli to detect and destroy Cholera, we demonstrated that E. coli is capable of sensing Cholera and hypothesize that it does so through its SdiA protein. In addition, we demonstrate induction of Lambda from lysogenic E. coli when exposed to Cholera.

Judging Criteria

Specifics

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 '''Phage Library'''
-- We were able to isolate mutant T7 bacteriophage that have a smaller average capsid size and less variability in capsid size when compared to the wild type.
+This summer, we developed a cesium chloride gradient protocol for isolating larger and smaller phage. Utilizing this gradient, we were able to isolate mutant T4 bacteriophages that have distinctively larger or smaller capsid. Similarly, we were also able to isolate mutant T7 bacteriophages that have smaller than average capsid size and less variability in capsid size when compared to the wild type.
-- We were able to isolate mutant T4 bacteriophage that produce larger and smaller plaques, suggesting they have altered capsid size.
-- We developed a cesium chloride gradient protocol for isolating larger and smaller phage.
 '''Cholera'''
-- We were able to show that ''E. coli'' is capable of sensing Cholera and hypothesize that it does so through its SdiA protein.
+To tackle the disease Cholera, we identified a protein, Amylase, that disrupt Cholera's biofilm formation. On our way to achieving our goals of engineering ''E. coli'' to detect and destroy Cholera, we demonstrated that ''E. coli'' is capable of sensing Cholera and hypothesize that it does so through its SdiA protein. In addition, we demonstrate induction of Lambda from lysogenic ''E. coli'' when exposed to Cholera.
-- We demonstrate induction of Lambda from lysogenic ''E. coli'' when exposed to Cholera.
-- We identified a protein, Amylase A, that disrupts Cholera's biofilm formation.
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