Template:Kyoto/Notebook/Aug 10

From 2013.igem.org

(Difference between revisions)
(→Liquid Culture)
(→LB Medium Plate(+Ampicillin))
 
Line 102: Line 102:
|0.4M NaOHaq||500µL
|0.4M NaOHaq||500µL
|-
|-
-
|Agarose Pouder||2g 
+
|Agarose Pouder||2g
|-
|-
|Ampicillin||40µL
|Ampicillin||40µL

Latest revision as of 03:40, 28 September 2013

Contents

Aug 10

Colony PCR

Nakamoto

Sample
8/9 BBa_J44000 -(1)
8/9 BBa_J44000 -(2)
NC
PreDenatureDenatureAnnealingExtensioncycle
94 °C94 °C55 °C68 °C--
5 min30 sec30 sec30 sec30 cycles
Sample
8/9 BBa_J23106
8/9 BBa_J23100 -(1)
8/9 BBa_J23100 -(2)
NC
PreDenatureDenatureAnnealingExtensioncycle
94 °C94 °C55 °C68 °C--
5 min30 sec30 sec1 min30 cycles

Electrophoresis

Tatsui and Nakamoto

LaneSample
100bp ladder
48/9 BBa_J44000 -1
58/9 BBa_J44000 -2
6NC

Igku Aug10 Electrophoresis BBa 1.jpg

LaneSample
1kbp ladder
18/9 BBa_J23106
28/9 BBa_J23100 -1
38/9 BBa_J23100 -2
4NC

Igku Aug10electrophoresis1.jpg

Liquid Culture

Nakamoto

Samplemedium
8/9 BBa-J23106LB(+Amp)
8/9 BBa-J23100-1LB(+Amp)
8/9 BBa-J23100-2LB(+Amp)
8/9 BBa-J44000-1LB(+Amp)
8/9 BBa-J44000-2LB(+Amp)

LB Medium Plate(+Ampicillin)

Nakamoto & Tatsui

volume200ml
Bacto T2ypton 2g
Bacto yeast extract 1g
NaCl1g
0.4M NaOHaq500µL
Agarose Pouder2g
Ampicillin40µL

Transformation

Hirano

NameSampleCompetent CellsTotalPlate
tetR3µL19µL22µLKan
RBS3µL19µL22µLAmp
lacZα3µL19µL22µLCP
lacI3µL19µL22µLCP
Plac3µL19µL22µLCP