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Team:Paris Bettencourt/Notebook/Trojan Horse/Wednesday 28th August.html
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Trojan Horse
ASDF28th August
Place your twit here
<IMG-plate xGAL control.jpg>
as expected MGZ1 and MG1655 turn blue.
Result from assemblies:
Gibson sRNA LacZ in litmus-GFP: failure
Biobrick assembly sRNA LacZ in Litmus-GFP : success
Biobrick assembly sRNA LacZ in pUC18 : success
Miniprep of Litmus-GFP-sRNACm ,Litmus-GFP-sRNALacZ, pUC18-sRNACm , pUC18-sRNALacZ
Building phagemid weapon producing cells: Transformation of electrocompetent MGZ1, F+ with M13K07 + Litmus-GFP-sRNALacZ / Litmus-GFP-sRNACm
Transforming:
-1) negative control : 5,2
-2) IDT plasmid with sRNALacZ ( should form white colonies of MGZ1 on Xgal+IPTG):5,1
-3) Litmus-GFP-sRNALacZ1 ( should form white colonies of MGZ1 on Xgal+IPTG): 5,2
-4) Litmus-GFP-sRNALacZ3 ( should form white colonies of MGZ1 on Xgal+IPTG): 5,2
-5) Litmus-GFP-sRNACm1 (control XGal ) : 5,2
-6) Litmus-GFP-sRNACm1 + M13K07: 5,2
-7) Litmus-GFP-sRNACm2 + M13K07 : 5,2
-8) Litmus-GFP-sRNACm3 + M13K07: 5,2
-9) Litmus-GFP-sRNALacZ1 + M13K07 : 5,2
-10) Litmus-GFP-sRNALacZ3 + M13K07: 5,2
plate on AMP+IPTG+Xgal ( silencing of LacZ on MGZ1 strain) and KAN+AMP (phage factories cells)
