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Team:Heidelberg/Templates/Del week10 pSB4K5
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(Created page with "==05-07-2013== ===Amplification from FS_01 to FS_16; 4.2 kb=== [[File:Heidelberg_20130705 DelFG DelBB DelFGsmall DelG.png|150px|thumb|Gel electrophoresis of DelF-G, Backbone, De...")
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(Created page with "==05-07-2013== ===Amplification from FS_01 to FS_16; 4.2 kb=== [[File:Heidelberg_20130705 DelFG DelBB DelFGsmall DelG.png|150px|thumb|Gel electrophoresis of DelF-G, Backbone, De...")
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Revision as of 01:14, 30 September 2013
05-07-2013
Amplification from FS_01 to FS_16; 4.2 kb
- Reaction
| what | µl |
|---|---|
| Template pSB4K5 | 1 |
| FS_16: (1/10) | 1 |
| FS_01: (1/10) | 1 |
| Phusion Master Mix | 10 |
| dd H2O | 6 |
| DMSO | 1 |
- Conditions
| Biorad MyCycler | ||
|---|---|---|
| Cycles-PCR | temperature [°C] | Time [s] |
| 1 | 98 | 5 |
| 14 | 98 | 1 |
| 62 ↓ 0.5 | 5 | |
| 72 | 3:20 min | |
| 16 | 98 | 1 |
| 60 | 5 | |
| 72 | 3:20 min | |
| 1 | 72 | 12 min |
| 1 | 4 | inf |
Results:
- Amplification worked well and band was at expected height. Nevertheless the gel band did not run properly on the gel.
