Exeter/19 July 2013

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==Transformations==
==Transformations==
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For this reason, we have been investigating other assembly methods. We have a few ideas on the go, but if you have any recommendations or tips, we'd love to hear them! You can always reach us through igem@ex.ac.uk.
For this reason, we have been investigating other assembly methods. We have a few ideas on the go, but if you have any recommendations or tips, we'd love to hear them! You can always reach us through igem@ex.ac.uk.
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Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook].
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Latest revision as of 07:10, 30 September 2013

Exeter iGEM 2013 · Paint by Coli

Transformations

We are transforming...

  • New Part 1 - CcaS (green light sensor) + terminator
  • New Part 2 - (promoter, RBS and FixJ) + terminator
  • New Part 3 - Yellow pigment + terminator
  • New Part 4 - Magenta pigment + terminator
  • New Part 7 - YF1 (blue light sensor) + terminator
  • New Part 8 - FixJ + terminator
  • Ligation control (RFP)
  • Retry of OmpR (BBa_K098011) from Kit Plate (failed on 16/7/13)
  • Retry of lambda repressor system (BBa_Q04510) from Kit Plate (failed on 16/7/13)
  • Different version of RBS + cph8 (red light sensor) from Kit Plate

(Monday morning, results of transformations)

Part Number of colonies
Different version of RBS + cph8 23
OmpR 120
Lambda repressor system 20 countable, other colonies visible but too small to numerate
RFP control 75
New Part 1 78
New Part 2 2
New Part 3 12
New Part 4 0
New Part 7 6
New Part 8 1
Ligation Control colonies present, too small to count, but approx. 100

Obviously, these results aren't fantastic. They are far better than our first attempt at digestions/ligations/transformations (we actually have colonies this time!) but until we can make liquid cultures and run some gels, we can't really know if they've worked.

For this reason, we have been investigating other assembly methods. We have a few ideas on the go, but if you have any recommendations or tips, we'd love to hear them! You can always reach us through igem@ex.ac.uk.

Take me back to the notebook.

Exeter iGEM 2013 · Paint by Coli
Retrieved from "http://2013.igem.org/Exeter/19_July_2013"