Exeter/5 August 2013

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=== Glycerol stocks ===
=== Glycerol stocks ===
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Two methods using 'standard' amounts of each digest and using equimolar amounts.
Two methods using 'standard' amounts of each digest and using equimolar amounts.
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Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook].
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Revision as of 07:21, 30 September 2013

Exeter iGEM 2013 · Paint by Coli

Contents

Glycerol stocks

We made up 3 replicates of:

K592018

S05058

B0015


Nano Drop

Culture NanoDrop concentration (ng/ul) size
K592018 160.7 2526bp
S05058 118.3 717bp
RFP 96.1 1047bp
B0015 76.3 129bp


Digestion

Part DNA (ul) dH20 (ul) Buffer (ul) BSA (ul) Enzyme 1 (ul) Enzyme 2 (ul) Enzyme 3 (ul)
K592018 1.6 14.4 2.5 0.5 EcoR1 0.5 Spe1 0.5 -
S05058 2.1 13.9 2.5 0.5 EcoR1 0.5 Spe1 0.5 -
B0015 3.3 12.7 2.5 0.5 Xbal 0.5 Pstl 0.5 -
AMP plasmid 10 6 2.5 0.5 EcoR1 0.5 Pst1 0.5 Dpnl 0.5
RFP 2.6 13.4 2.5 0.5 EcoR1 0.5 Pstl 0.5 -


Ligation

Two methods using 'standard' amounts of each digest and using equimolar amounts.

Take me back to the notebook.

Exeter iGEM 2013 · Paint by Coli
Retrieved from "http://2013.igem.org/Exeter/5_August_2013"