Team:MIT

From 2013.igem.org

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This is a template page. READ THESE INSTRUCTIONS.
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace. 
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This year, the MIT iGEM team is working to develop circuits that implement multiplexed cell-cell communication mediated by exosomes in mammalian cells. Our approach is to incorporate two parallel signaling strategies using exosomes: small miRNA and a Cas9 complex.
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In the first strategy, we utilize miRNA that are selectively targeted into exosomes. Sender cells produce exosomes with our miRNA signals. These exosomes carry signals to engineered receiver cells that use these miRNA inputs to modulate gene expression.
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The second signaling strategy employs proteins contained within exosomes. We fuse targeting motifs to a CAS9-VP16 protein resulting in selective exosomal partitioning of this species in sender cells. In receiver cells, this signal modulates gene expression through the Cas9-CRISPR mechanism with a variable guide RNA.
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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| width=20% | [[Image:MIT_team.png|300px|Top|MIT Full Team]]
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:MIT_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:MIT | Team MIT]]
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Revision as of 03:59, 5 July 2013

MIT logo.png

This year, the MIT iGEM team is working to develop circuits that implement multiplexed cell-cell communication mediated by exosomes in mammalian cells. Our approach is to incorporate two parallel signaling strategies using exosomes: small miRNA and a Cas9 complex.

In the first strategy, we utilize miRNA that are selectively targeted into exosomes. Sender cells produce exosomes with our miRNA signals. These exosomes carry signals to engineered receiver cells that use these miRNA inputs to modulate gene expression.

The second signaling strategy employs proteins contained within exosomes. We fuse targeting motifs to a CAS9-VP16 protein resulting in selective exosomal partitioning of this species in sender cells. In receiver cells, this signal modulates gene expression through the Cas9-CRISPR mechanism with a variable guide RNA.

MIT Full Team


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