Team:EPF Lausanne/Calendar/1 September 2013

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Revision as of 18:35, 30 September 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Sensing

Suspending Primers
Our new primers arrived and we resuspended them and made primer mixes for each pair, one foreward and one reverse.

Suspending Primers
The AraC-GFP containing bacteria were made to express GFP by adding 1% of 20% L-Arabinose. After adding the Arabinose we incubated them for 2.5h at 37°C and then looked at them in the microscope. They clearly fluoresce, indicating that GFP is expressed induced by addition of Arabinose to the medium.