GigiGoesGugu (Talk | contribs) (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Suspending Primers''' <BR> Our new primers arrived and we resuspended them and made primer mixes for each pair, one foreward and one...") |
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Our new primers arrived and we resuspended them and made primer mixes for each pair, one foreward and one reverse. | Our new primers arrived and we resuspended them and made primer mixes for each pair, one foreward and one reverse. | ||
| - | ''' | + | '''Inducing and measuring GFP expression''' <BR> |
The AraC-GFP containing bacteria were made to express GFP by adding 1% of 20% L-Arabinose. After adding the Arabinose we incubated them for 2.5h at 37°C and then looked at them in the microscope. They clearly fluoresce, indicating that GFP is expressed induced by addition of Arabinose to the medium. | The AraC-GFP containing bacteria were made to express GFP by adding 1% of 20% L-Arabinose. After adding the Arabinose we incubated them for 2.5h at 37°C and then looked at them in the microscope. They clearly fluoresce, indicating that GFP is expressed induced by addition of Arabinose to the medium. | ||
{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
Sensing
Suspending Primers
Our new primers arrived and we resuspended them and made primer mixes for each pair, one foreward and one reverse.
Inducing and measuring GFP expression
The AraC-GFP containing bacteria were made to express GFP by adding 1% of 20% L-Arabinose. After adding the Arabinose we incubated them for 2.5h at 37°C and then looked at them in the microscope. They clearly fluoresce, indicating that GFP is expressed induced by addition of Arabinose to the medium.
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