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Team:Heidelberg/Templates/Del week14 G
From 2013.igem.org
(Difference between revisions)
(Created page with "==29-07-2013== ===Restriction digest from FS_08 to FS_23; 6.5 kb; 23-07-2013 with ClaI=== [[File:Heidelberg_20130729restrictiondigest 2log FS02-FS03 FS02-FS05...") |
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| dd H<sub>2</sub>O || 2 | | dd H<sub>2</sub>O || 2 | ||
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| + | | Expected fragment lengths [bp] || 3291, 1936, 1303 | ||
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Revision as of 20:15, 30 September 2013
29-07-2013
Restriction digest from FS_08 to FS_23; 6.5 kb; 23-07-2013 with ClaI
Incubation at 37°C for about 3 hours
| what | µl |
|---|---|
| FS_08 to FS_23 (2) (23-07-2013) | 15 |
| ClaI | 1 |
| Buffer CutSmart | 2 |
| dd H2O | 2 |
| Expected fragment lengths [bp] | 3291, 1936, 1303 |
Results:
- Restriction digest of DelG (FS_08 to FS_23) was successful all predicted bands are clearly visible
