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Team:Heidelberg/Templates/Del week14 G
From 2013.igem.org
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==29-07-2013== | ==29-07-2013== | ||
| - | ===Restriction digest | + | ===Restriction digest of fragment FS_08 to FS_23; 6.5 kb; [[DelG#23-07-2013|23-07-2013]] with ClaI=== |
[[File:Heidelberg_20130729restrictiondigest 2log FS02-FS03 FS02-FS05 FS08-FS23besch.png|150px|thumb|Test restriction digest (29.07); run at 100 V, 0.8 % gel (TAE)]] | [[File:Heidelberg_20130729restrictiondigest 2log FS02-FS03 FS02-FS05 FS08-FS23besch.png|150px|thumb|Test restriction digest (29.07); run at 100 V, 0.8 % gel (TAE)]] | ||
Revision as of 20:15, 30 September 2013
29-07-2013
Restriction digest of fragment FS_08 to FS_23; 6.5 kb; 23-07-2013 with ClaI
Incubation at 37°C for about 3 hours
| what | µl |
|---|---|
| FS_08 to FS_23 (2) (23-07-2013) | 15 |
| ClaI | 1 |
| Buffer CutSmart | 2 |
| dd H2O | 2 |
| Expected fragment lengths [bp] | 3291, 1936, 1303 |
Results:
- Restriction digest of DelG (FS_08 to FS_23) was successful all predicted bands are clearly visible
