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Cell Surface DisplayPCR optimisation: to improve the pINP_construct amplification we did a gradient PCR at 72, 73 and 74°C with 3% and 5% DMSO added to the mix. we verified our results with a 0.8% gel electrophoresis.
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+ | '''Cell Surface Display''' | ||
+ | ''PCR optimisation:'' to improve the ''pINP_construct'' amplification we did a gradient PCR at 72, 73 and 74°C with 3% and 5% DMSO added to the mix. | ||
+ | we verified our results with a 0.8% gel electrophoresis. | ||
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PCR optimisation: to improve the pINP_construct amplification we did a gradient PCR at 72, 73 and 74°C with 3% and 5% DMSO added to the mix. we verified our results with a 0.8% gel electrophoresis.